Strategies of functionalized GelMA-based bioinks for bone regeneration: Recent advances and future perspectives

Bioactive Materials, Journal Year: 2024, Volume and Issue: 38, P. 346 - 373

Published: May 9, 2024

Gelatin methacryloyl (GelMA) hydrogels is a widely used bioink because of its good biological properties and tunable physicochemical properties, which has been in variety tissue engineering regeneration. However, pure GelMA limited by the weak mechanical strength lack continuous osteogenic induction environment, difficult to meet needs bone repair. Moreover, are unable respond complex stimuli therefore adapt physiological pathological microenvironments. This review focused on functionalization strategies hydrogel based bioinks for The synthesis process was described details, various functional methods requirements regeneration, including strength, porosity, vascularization, differentiation, immunoregulation patient specific repair, etc. In addition, response smart GelMA-based external physical stimulation internal microenvironment stimulation, as well achieve both disease treatment regeneration presence common diseases (such inflammation, infection, tumor) also briefly reviewed. Finally, we emphasized current challenges possible exploration directions

Language: Английский

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Three‐dimensional bioprinting biphasic multicellular living scaffold facilitates osteochondral defect regeneration

Interdisciplinary materials, Journal Year: 2024, Volume and Issue: 3(5), P. 738 - 756

Published: June 2, 2024

Abstract Due to tissue lineage variances and the anisotropic physiological characteristics, regenerating complex osteochondral tissues (cartilage subchondral bone) remains a great challenge, which is primarily due distinct requirements for cartilage bone regeneration. For regeneration, significant amount of newly generated chondrocytes required while maintaining their phenotype. Conversely, regeneration necessitates inducing stem cells differentiate into osteoblasts. Additionally, construction interface crucial. In this study, we fabricated biphasic multicellular bioprinted scaffold mimicking natural employing three‐dimensional (3D) bioprinting technology. Briefly, gelatin‐methacryloyl (GelMA) loaded with articular marrow mesenchymal (ACs/BMSCs), serving as layer, preserved phenotype ACs promoted differentiation BMSCs through interaction between BMSCs, thereby facilitating GelMA/strontium‐substituted xonotlite (Sr‐CSH) regulated osteoblasts enhanced secretion matrix by in layer slow release bioactive ions from Sr‐CSH. GelMA, material, contributed reconstruction interface. Ultimately, demonstrated satisfactory simultaneous defects. promising strategy application 3D technology was proposed.

Language: Английский

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An injectable multi-functional composite bioactive hydrogel for bone regeneration via immunoregulatory and osteogenesis effects

Advanced Composites and Hybrid Materials, Journal Year: 2025, Volume and Issue: 8(1)

Published: Jan. 17, 2025

Language: Английский

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Droplet Microfluidics Powered Hydrogel Microparticles for Stem Cell‐Mediated Biomedical Applications

Small, Journal Year: 2024, Volume and Issue: unknown

Published: June 16, 2024

Abstract Stem cell‐related therapeutic technologies have garnered significant attention of the research community for their multi‐faceted applications. To promote effects stem cells, strategies cell microencapsulation in hydrogel microparticles been widely explored, as potential to facilitate oxygen diffusion and nutrient transport alongside ability crucial cell‐cell cell‐matrix interactions. Despite promise, there is an acute shortage automated, standardized, reproducible platforms further research. Microfluidics offers intriguing platform produce cell‐laden (SCHMs) owing its manipulate fluids at micrometer scale well precisely control structure composition microparticles. In this review, typical biomaterials crosslinking methods microfluidic encapsulation cells progress droplet‐based microfluidics fabrication SCHMs are outlined. Moreover, important biomedical applications highlighted, including regenerative medicine, tissue engineering, scale‐up production microenvironmental simulation fundamental studies. Overall, holds tremendous enabling diverse worthy various

Language: Английский

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A review of sodium alginate-based hydrogels: Structure, mechanisms, applications, and perspectives

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: unknown, P. 139151 - 139151

Published: Dec. 1, 2024

Language: Английский

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Divalent metal ions enhance bone regeneration through modulation of nervous systems and metabolic pathways

Bioactive Materials, Journal Year: 2025, Volume and Issue: 47, P. 432 - 447

Published: Feb. 12, 2025

Language: Английский

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Recent Progress in Alginate-based Nanocomposites for Bone Tissue Engineering Applications

Colloids and Surfaces B Biointerfaces, Journal Year: 2025, Volume and Issue: 250, P. 114570 - 114570

Published: Feb. 18, 2025

Language: Английский

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Magnetically responsive micro-clustered calcium phosphate-reinforced cell-laden microbead sodium alginate hydrogel for accelerated osteogenic tissue regeneration

Carbohydrate Polymers, Journal Year: 2024, Volume and Issue: 346, P. 122666 - 122666

Published: Aug. 28, 2024

Language: Английский

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Injectable osteogenic hydrogel based on antibiotic recognition for infected radial bone defect regeneration

Materials & Design, Journal Year: 2024, Volume and Issue: 241, P. 112904 - 112904

Published: March 31, 2024

Bone defects induced by trauma, infection, and tumors present a significant challenge for treatment in clinical trials. Biomaterial-based tissue engineering strategy has shown great promise bone defect repair. However, multiple functions inherent the extracellular matrix (ECM) are rarely covered design of scaffold materials associated with grafting procedures. These deficiencies significantly limit its potential application pathologic therapy. In this work, we constructed hydrogel mimicking dynamic features ECM combination antimicrobial osteogenic capabilities as well, to investigate applications therapy infected radial defects. The enables not only minimally invasive implantation but also adaptability adhesion irregular shapes. Both vitro vivo assays have indicated that could rapidly eradicate bacteria, restore infection-induced localized inflammatory microenvironment. Further loading growth peptide (OGP) recruit stem cells promotes differentiation ultimately enhances regeneration region. summary, our work hydrogels ECM-related bioactivities inner structures represent feasible solution bionic substitute

Language: Английский

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Autonomous living materials and bone-inspired scaffolds motivated by human osteogenic microenvironment mechanisms

Materials & Design, Journal Year: 2024, Volume and Issue: 244, P. 113145 - 113145

Published: July 8, 2024

The repair of secondary critical bone defects is an international medical challenge. Bone tissue engineering provides methods and technology for repair. regeneration mechanism serves as inspiration the material structural design scaffolds. In terms materials, this review draws from biological characteristics host cells in osteogenic microenvironment (including osteoblast lineage, vascular cell inflammatory cells, etc.), reviewing regulatory mechanisms self-healing proposing autonomous living materials scaffolds which prepared by in-situ manufacturing. Autonomous regulate migration, proliferation differentiation real time releasing steadily long-term. Regarding structure, we functional role natural structures homeostasis, providing insights into bone-inspired Due to conflict between mechanical properties ability, proposes assembled They can prolong half-life provide support attachment points new growth, autonomously microenvironment. have potential advance research progress field pave way novel clinical treatments.

Language: Английский

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