lncRNA JPX/miR-33a-5p/Twist1 axis regulates tumorigenesis and metastasis of lung cancer by activating Wnt/β-catenin signaling DOI Creative Commons

Jinchang Pan,

Shuai Fang,

Haihua Tian

et al.

Molecular Cancer, Journal Year: 2020, Volume and Issue: 19(1)

Published: Jan. 15, 2020

MicroRNAs (miRNAs) and Twist1-induced epithelial-mesenchymal transition (EMT) in cancer cell dissemination are well established, but the involvement of long noncoding RNAs (lncRNAs) Twist1-mediated signaling remains largely unknown.RT-qPCR western blotting were conducted to detect expression levels lncRNA JPX Twist1 lung lines tissues. The impact on expression, growth, invasion, apoptosis, vivo tumor growth investigated cells by blotting, rescue experiments, colony formation assay, flow cytometry, xenograft animal experiment.We observed that was upregulated metastatic tissues closely correlated with size an advanced stage. Functionally, promoted proliferation vitro facilitated vivo. Additionally, competitively sponging miR-33a-5p subsequently induced EMT invasion. Interestingly, coordinately cells. Mechanically, JPX/miR-33a-5p/Twist1 axis participated progression activating Wnt/β-catenin signaling.These findings suggest JPX, a mediator signaling, could predispose metastasis may serve as potential target for targeted therapy.

Language: Английский

The functions and unique features of long intergenic non-coding RNA DOI
Julia D. Ransohoff,

Yuning Wei,

Paul A. Khavari

et al.

Nature Reviews Molecular Cell Biology, Journal Year: 2017, Volume and Issue: 19(3), P. 143 - 157

Published: Nov. 15, 2017

Language: Английский

Citations

1166
Long non-coding RNAs: definitions, functions, challenges and recommendations DOI Open Access
John S. Mattick, Paulo Amaral, Piero Carninci

et al.

Nature Reviews Molecular Cell Biology, Journal Year: 2023, Volume and Issue: 24(6), P. 430 - 447

Published: Jan. 3, 2023

Language: Английский

Citations

1157
Noncoding RNA therapeutics — challenges and potential solutions DOI Open Access
Melanie Winkle, Sherien M. El‐Daly, Muller Fabbri

et al.

Nature Reviews Drug Discovery, Journal Year: 2021, Volume and Issue: 20(8), P. 629 - 651

Published: June 18, 2021

Language: Английский

Citations

1136
TGF-β-Mediated Epithelial-Mesenchymal Transition and Cancer Metastasis DOI Open Access
Hao Yang, D.A. Baker, Peter ten Dijke

et al.

International Journal of Molecular Sciences, Journal Year: 2019, Volume and Issue: 20(11), P. 2767 - 2767

Published: June 5, 2019

Transforming growth factor β (TGF-β) is a secreted cytokine that regulates cell proliferation, migration, and the differentiation of plethora different types. Consistent with these findings, TGF-β plays key role in controlling embryogenic development, inflammation, tissue repair, as well maintaining adult homeostasis. elicits broad range context-dependent cellular responses, consequently, alterations signaling have been implicated many diseases, including cancer. During early stages tumorigenesis, acts tumor suppressor by inducing cytostasis apoptosis normal premalignant cells. However, at later stages, when cancer cells acquired oncogenic mutations and/or lost gene function, are resistant to TGF-β-induced arrest, functions promotor stimulating undergo so-called epithelial-mesenchymal transition (EMT). The latter leads metastasis chemotherapy resistance. further supports progression activating angiogenesis cancer-associated fibroblasts enabling evade inhibitory immune responses. In this review, we will consider cycle apoptosis, EMT metastasis. particular, highlight recent insights into multistep dynamically controlled process miRNAs long noncoding RNAs process. Finally, discuss how new mechanistic might be exploited develop novel therapeutic interventions.

Language: Английский

Citations

858
Carcinoma-associated fibroblasts promote the stemness and chemoresistance of colorectal cancer by transferring exosomal lncRNA H19 DOI Creative Commons
Jing Ren, Liang Ding, Dongya Zhang

et al.

Theranostics, Journal Year: 2018, Volume and Issue: 8(14), P. 3932 - 3948

Published: Jan. 1, 2018

Long non-coding RNAs (lncRNAs) are involved in the pathology of various tumors, including colorectal cancer (CRC). The crosstalk between carcinoma- associated fibroblasts (CAFs) and cells tumor microenvironment promotes development confers chemoresistance. In this study, we further investigated underlying tumor-promoting roles CAFs molecular mediators these processes. Methods: AOM/DSS-induced colitis-associated (CAC) mouse model was established, RNA sequencing performed. Small interfering (siRNA) sequences were used to knock down H19. Cell apoptosis measured by flow cytometry. SW480 with H19 stably knocked establish a xenograft model. indicated protein levels tissues confirmed immunohistochemistry assay, cell analyzed TUNEL assay. RNA-FISH immunofluorescence assays performed assess expression stroma nests. AldeRed ALDH detection assay detect intracellular aldehyde dehydrogenase (ALDH) enzyme activity. Isolated exosomes identified transmission electron microscopy, nanoparticle tracking Western blotting. Results: highly expressed CAC mice compared normal colon tissues. up-regulation also CRC patient samples at different node metastasis (TNM) stages. Moreover, stemness stem (CSCs) specimens. promoted CSCs increased frequency tumor-initiating cells. showed higher than Of note, enriched CAF-derived conditioned medium exosomes, which turn chemoresistance vitro vivo. Furthermore, activated β-catenin pathway via acting as competing endogenous sponge for miR-141 CRC, while significantly inhibited Conclusion: promote transferring exosomal miR-141, Our findings indicate that contributes

Language: Английский

Citations

587
Long noncoding RNAs in cancer metastasis DOI
Siyuan Liu, Ha X. Dang, Daniel A. Lim

et al.

Nature reviews. Cancer, Journal Year: 2021, Volume and Issue: 21(7), P. 446 - 460

Published: May 5, 2021

Language: Английский

Citations

503
Biomarker development in the precision medicine era: lung cancer as a case study DOI
Ashley J. Vargas, Curtis C. Harris

Nature reviews. Cancer, Journal Year: 2016, Volume and Issue: 16(8), P. 525 - 537

Published: July 8, 2016

Language: Английский

Citations

485
Long noncoding RNA LINC00336 inhibits ferroptosis in lung cancer by functioning as a competing endogenous RNA DOI Creative Commons
Min Wang, Chao Mao,

Lianlian Ouyang

et al.

Cell Death and Differentiation, Journal Year: 2019, Volume and Issue: 26(11), P. 2329 - 2343

Published: Feb. 20, 2019

The regulatory loop between long noncoding RNAs (lncRNAs) and microRNAs has a dynamic role in transcriptional translational regulation, is involved cancer. However, the circuitry lncRNAs tumorigenesis remains elusive. Here we demonstrate that nuclear lncRNA LINC00336 upregulated lung cancer functions as an oncogene by acting competing endogenous RNA (ceRNAs). bound RNA-binding protein ELAVL1 (ELAV-like 1) using nucleotides 1901–2107 of RRM interaction domain key amino acids (aa) (aa 101–213), inhibiting ferroptosis. Moreover, increased expression stabilizing its posttranscriptional level, whereas LSH (lymphoid-specific helicase) through p53 signaling pathway, further supporting hypothesis promotes expression. Interestingly, served sponge microRNA 6852 (MIR6852) to regulate cystathionine-β-synthase (CBS), surrogate marker Finally, found MIR6852 inhibited cell growth promoting These data show network ceRNA important

Language: Английский

Citations

484
Promoter of lncRNA Gene PVT1 Is a Tumor-Suppressor DNA Boundary Element DOI Creative Commons
Seung Woo Cho, Jin Xu, Ruping Sun

et al.

Cell, Journal Year: 2018, Volume and Issue: 173(6), P. 1398 - 1412.e22

Published: May 1, 2018

Language: Английский

Citations

423
lncRNA Epigenetic Landscape Analysis Identifies EPIC1 as an Oncogenic lncRNA that Interacts with MYC and Promotes Cell-Cycle Progression in Cancer DOI Creative Commons
Zehua Wang, Bo Yang, Min Zhang

et al.

Cancer Cell, Journal Year: 2018, Volume and Issue: 33(4), P. 706 - 720.e9

Published: April 1, 2018

We characterized the epigenetic landscape of genes encoding long noncoding RNAs (lncRNAs) across 6,475 tumors and 455 cancer cell lines. In stark contrast to CpG island hypermethylation phenotype in cancer, we observed a recurrent hypomethylation 1,006 lncRNA including EPIC1 (epigenetically-induced lncRNA1). Overexpression is associated with poor prognosis luminal B breast patients enhances tumor growth vitro vivo. Mechanistically, promotes cell-cycle progression by interacting MYC through EPIC1's 129–283 nt region. knockdown reduces occupancy its target (e.g., CDKN1A, CCNA2, CDC20, CDC45). depletion abolishes regulation tumorigenesis

Language: Английский

Citations

411