Mitochondria–Nucleus Migration Probe for Ultrasensitive Monitoring of mtDNA Damage in Living Cells DOI

Zhen‐Qing Yu,

Wenjing Pan, Xiao‐Feng Yang

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 97(1), P. 584 - 593

Published: Dec. 31, 2024

Mitochondrial DNA (mtDNA) damage is a prevalent phenomenon that has been proven to be implicated in wide spectrum of diseases. However, the progressive attenuation probe signals response mtDNA within living cells inherently limits sensitivity and precision current probes for detecting damage. Herein, we employ an innovative organelle signal ratio imaging approach, utilizing mitochondria–nucleus migration MCQ, achieve unparalleled cells. MCQ exhibited initial preferential binding mtDNA, facilitated by its cationic quinolinium moiety, but migrated nucleus upon This unique behavior not only enhanced spatial identifiability also amplified detection significantly harnessing intensified against attenuated mitochondrial signal. approach established positive correlation between damage, enabling even subtle at early stage apoptosis with remarkable 23-fold enhancement following just 5 min H2O2 induction cells, whereas conventional methods relying solely on fading proved insufficient. Furthermore, MCQ's ability monitor occurrence achieved intricate differentiation ferroptosis. By monitoring drug-induced cancer was further conducted using evaluate therapeutic efficacy four anticancer drugs very low concentrations. strategy paves way ultrasensitive holds immense promise damage-associated

Language: Английский

A multifunctional fluorescent probe for sequential detection of hydrogen sulfide and pH in foodstuffs, living cells and mice DOI
Zu‐Sheng Huang, Wenxuan Zhang,

Manshan Liang

et al.

Analytica Chimica Acta, Journal Year: 2024, Volume and Issue: 1299, P. 342434 - 342434

Published: Feb. 28, 2024

Language: Английский

Citations

13
A single excitation dual emission semi-salamo type multi-functional probe for sensitive pH and Cu2+ detection DOI
Wenting Guo,

Dongzhen Bai,

Yunzhao He

et al.

Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy, Journal Year: 2024, Volume and Issue: 318, P. 124386 - 124386

Published: May 3, 2024

Language: Английский

Citations

3
De Novo Construction of pKa‐Tunable Xanthene Molecules for pH Sensitive Fluorescence Navigation DOI Open Access
Lina Zhang, Shan‐Yong Chen, Lei Shi

et al.

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 29, 2024

Abstract pH is spatiotemporal variable dynamically in physiological and pathological processes, the tiny changes can result completely different processes. However, major drawbacks of conventional pH‐activatable probes are their large acid‐base transition width challenge to adjust pKa suitable for various application. In response these issues, a novel approach proposed realize sensitive general strategy construct multicolor pKa‐tunable xanthene‐based platform. probes, narrowest range just 1 units, achieved by introducing electron‐rich pyridine as ingenious proton catcher. Especially, Si‐4Py owns near‐infrared (NIR) signal 5.56, which successfully used rapid high‐contrast imaging distal metastatic tumors. This has significant implications guiding surgeons removal tumors ultimately improve patient outcomes. Additional, sustainably innovative this work serve effective reference probe development.

Language: Английский

Citations

3
Next-generation Red Ultra-bright Fluorescent Dyes for Nuclear Imaging and Peripheral Blood Leukocytes Sorting DOI Creative Commons
Zipeng Li, Liu Zheng, Dinghua Yu

et al.

Chemical Science, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

The red ultra-bright nuclear-targeted dye BPC1 was developed. exhibits ultrahigh fluorescence brightness and exceptional cell permeability, enabling the visualization of nucleus by substantially lower doses (100 nM) laser power.

Language: Английский

Citations

0
Mitochondria–Nucleus Migration Probe for Ultrasensitive Monitoring of mtDNA Damage in Living Cells DOI

Zhen‐Qing Yu,

Wenjing Pan, Xiao‐Feng Yang

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 97(1), P. 584 - 593

Published: Dec. 31, 2024

Mitochondrial DNA (mtDNA) damage is a prevalent phenomenon that has been proven to be implicated in wide spectrum of diseases. However, the progressive attenuation probe signals response mtDNA within living cells inherently limits sensitivity and precision current probes for detecting damage. Herein, we employ an innovative organelle signal ratio imaging approach, utilizing mitochondria–nucleus migration MCQ, achieve unparalleled cells. MCQ exhibited initial preferential binding mtDNA, facilitated by its cationic quinolinium moiety, but migrated nucleus upon This unique behavior not only enhanced spatial identifiability also amplified detection significantly harnessing intensified against attenuated mitochondrial signal. approach established positive correlation between damage, enabling even subtle at early stage apoptosis with remarkable 23-fold enhancement following just 5 min H2O2 induction cells, whereas conventional methods relying solely on fading proved insufficient. Furthermore, MCQ's ability monitor occurrence achieved intricate differentiation ferroptosis. By monitoring drug-induced cancer was further conducted using evaluate therapeutic efficacy four anticancer drugs very low concentrations. strategy paves way ultrasensitive holds immense promise damage-associated

Language: Английский

Citations

0