Journal of the American Chemical Society,
Journal Year:
2025,
Volume and Issue:
unknown
Published: April 25, 2025
Excited-state
intramolecular
proton
transfer
(ESIPT)
fluorophores
are
valuable
for
ratiometric
bioimaging
due
to
their
microenvironmental
sensitivity,
but
traditional
enol-keto
systems
suffer
from
poor
biocompatibility
and
reduced
efficiency
in
polar,
protic
environments.
Here,
we
introduce
a
tail-assisted
ESIPT
(ta-ESIPT)
strategy
which
occurs
an
amide
donor
amino
nitrogen
acceptor.
This
mechanism
applies
biocompatible
charge-transfer
fluorophores,
such
as
naphthalimide,
coumarin,
NBD,
acedan.
ta-ESIPT
exhibit
broad
environmental
stability
hydration-gated
response─proton
is
inhibited
aqueous
environments
restored
nonaqueous
microenvironments,
yielding
red-shifted
emission.
property
enables
the
precise
visualization
of
biomolecular
interactions.
By
conjugating
with
protein
ligands,
achieve
precise,
imaging
targets
like
SNAP-tag,
hCA,
avidin,
HaloTag
live
cells,
fluorescence
signals
that
directly
correlate
binding
affinities.
correlation
real-time
monitoring
interactions
evaluation
inhibitors
while
minimizing
nonspecific
interference
complex
cellular
environment,
ensuring
dynamic
accurate
recognition.
ACS Sensors,
Journal Year:
2022,
Volume and Issue:
7(9), P. 2521 - 2536
Published: Sept. 1, 2022
Fluorescence
sensing
can
not
only
identify
a
target
substrate
qualitatively
but
also
achieve
the
purpose
of
quantitative
detection
through
change
fluorescence
signal.
It
has
advantages
immense
sensitivity,
rapid
response,
and
excellent
selectivity.
The
proposed
aggregation-induced
emission
(AIE)
concept
solves
problem
traditional
fluorescent
molecules
becoming
weak
or
quenched
in
high
concentration
aggregated
state
conditions.
Schiff
base
probes
have
simple
synthesis,
low
toxicity,
easy
design.
They
are
often
used
for
various
substances.
In
this
review
we
cover
late
developments
compounds
with
AIE
characteristics
working
as
sensors.
ACS Omega,
Journal Year:
2022,
Volume and Issue:
7(37), P. 33248 - 33257
Published: Sept. 12, 2022
A
pyrene-based
fluorescent
chemosensor
APSB
[N-(pyrene-1-ylmethylene)
anthracen-2-amine]
was
designed
and
developed
by
a
simple
condensation
reaction
between
pyrene
carboxaldehyde
2-aminoanthracene.
The
sensor
selectively
binds
Fe3+
in
the
presence
of
other
metal
ions.
Apart
from
this,
shows
high
selectivity
sensitivity
toward
ion
detection.
detection
limit
for
1.95
nM,
binding
constant
(Kb)
obtained
as
8.20
×
105
M-1
DMSO/water
(95/5,
v/v)
medium.
fluorescence
quantum
yields
APSB-Fe3+
were
calculated
0.035
0.573,
respectively.
function
this
can
be
explained
through
photo-induced
electron
transfer
mechanism
which
further
proved
density
functional
theory
studies.
Finally,
live-cell
image
study
HeLa
cells
also
carried
out
to
investigate
cell
permeability
its
efficiency
selective
living
cells.
Molecules,
Journal Year:
2023,
Volume and Issue:
28(10), P. 4252 - 4252
Published: May 22, 2023
Biothiols
such
as
cysteine,
homocysteine,
and
glutathione
play
significant
roles
in
important
biological
activities,
their
abnormal
concentrations
have
been
found
to
be
closely
associated
with
certain
diseases,
making
detection
a
critical
task.
To
this
end,
fluorescent
probes
become
increasingly
popular
due
numerous
advantages,
including
easy
handling,
desirable
spatiotemporal
resolution,
high
sensitivity,
fast
response,
favorable
biocompatibility.
As
result,
intensive
research
has
conducted
create
for
the
imaging
of
biothiols.
This
brief
review
summarizes
recent
advances
field
biothiol-responsive
probes,
an
emphasis
on
rational
probe
design,
reaction
mechanism,
discriminating
detection,
reversible
specific
detection.
Furthermore,
challenges
prospects
fluorescence
biothiols
are
also
outlined.
