TrAC Trends in Analytical Chemistry, Journal Year: 2023, Volume and Issue: 167, P. 117270 - 117270
Published: Sept. 6, 2023
Language: Английский
Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown
Published: May 7, 2025
Circular RNAs (circRNAs) are noncoding with covalently closed circular structures that regulate important cellular processes, and their dysregulation is implicated in the pathogenesis progression of various cancers. Simultaneous specific detection multiple circRNAs significant importance early diagnosis cancer. Herein, we develop a ligation-controlled single-molecule biosensor for multiplexed measurement breast cancer-associated circRNAs. This assay integrates isothermal exponential amplification reaction (EXPAR)-induced generation DNAzymes Au nanoparticle (AuNP)-based spherical nucleic acid nanoprobe. The back-splice junction (BSJ) sequences circFOXO3 circMTO1 can serve as templates to ligate hairpin probes helper under catalysis SplintR ligase, forming complete templates. Afterward, ligated template both primer initiate EXPAR, inducing accumulation characteristic DNAzyme (i.e., 1 2). 2 be paired signal immobilized on AuNP surface, respectively, cyclic degradation liberate large amounts Cy5 Cy3 fluorophores achieving limits 8.34 aM 9.84 circMTO1. has been successfully applied simultaneous analysis single cancer cell differentiation circRNA levels between tissues healthy para-carcinoma tissues, offering new paradigm biomedical research circRNA-related molecular diagnostics.
Language: Английский
Citations
0
TrAC Trends in Analytical Chemistry, Journal Year: 2023, Volume and Issue: 164, P. 117085 - 117085
Published: May 6, 2023
Language: Английский
Citations
9
Advanced Sensor Research, Journal Year: 2023, Volume and Issue: 2(3)
Published: Jan. 1, 2023
Abstract Metabolic biomarkers facilitate pathological/physiological investigations and clinic decisions. Noninvasive quantification of metabolic allows patients to monitor their health without time, location, environmental constraints. Recently, the synergistic integration between high‐throughput screening strategy via mass spectrometry noninvasive techniques wearable biosensors may provide fundamental insights into application in clinical diagnostics. Here, for several major diseases (retinoblastoma, coronary heart disease, lung adenocarcinoma) is introduced. Then, three types based on electrochemistry, organic electrochemical transistors, field effect transistors are summarized. The review serve as a handbook metabolites. It anticipated that biomarker discovery will next generation toward personalized, intelligent, precise home–healthcare large‐scale.
Language: Английский
Citations
8
IEEE Sensors Journal, Journal Year: 2024, Volume and Issue: 24(9), P. 14101 - 14108
Published: March 14, 2024
This article proposed a trident-shaped fiber-optic WaveFlex biosensor based on the localized surface plasmon resonance (LSPR) principle for detection of ochratoxin A (OTA). The LSPR phenomenon can be successfully excited by coating probe with gold nanoparticles (AuNPs). In order to obtain more pronounced phenomenon, WaveFlex-type sensor was fabricated using single-mode fiber (SMF), which enhance evanescent waves (EWs) and generate stronger field better excite phenomenon. Since is sensitive dielectric environment surrounding environment, multiwalled carbon nanotubes (MWCNTs) tungsten disulfide quantum dots (WS2-QDs) are used change AuNPs regulate improve sensing performance sensor. To evaluate sensor, tests such as repeatability, reproducibility, stability, selectivity were conducted probe. linear range 0–60 ng/mL, limit 4.45 ng/mL. Finally, OTA in corn beer showed recovery rates 84.6%–100% 84.5%–92.1%, respectively. experimental results indicate that has excellent potential application real food samples.
Language: Английский
Citations
3
TrAC Trends in Analytical Chemistry, Journal Year: 2023, Volume and Issue: 167, P. 117270 - 117270
Published: Sept. 6, 2023
Language: Английский
Citations
7