Chemistry - A European Journal,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Dec. 10, 2024
Abstract
The
development
of
redox‐sensitive
molecular
fluorescent
probes
for
the
detection
redox
changes
in
Plasmodium
falciparum
‐parasitized
red
blood
cells
remains
interest
due
to
limitations
current
genetically
encoded
biosensors.
This
study
describes
design,
screening
and
synthesis
new
pro‐fluorophores
based
on
flavylium
azido
dyes
coupled
by
CuAAC
click
chemistry
alkynyl
analogues
plasmodione
oxide,
key
metabolite
potent
redox‐active
antimalarial
plasmodione.
photophysical
electrochemical
properties
these
were
evaluated,
focusing
their
fluorogenic
responses.
influence
both
status
quinone
length
PEG
chain
separating
fluorophore
from
electrophore
was
investigated.
fluorescence
quenching
photoinduced
electron
transfer
is
reversible
high
amplitude
oxidized
forms
reinstated
reduced
hydroquinone
forms.
Our
results
demonstrate
that
shortening
has
effect
enhancing
response,
likely
non‐covalent
interactions
between
two
chromophores.
All
systems
evaluated
antiparasitic
activities
imaging
suggests
efficacy
P.
cells,
paving
way
future
parasite
studies
monitor
cellular
processes.
Analytical Chemistry,
Journal Year:
2025,
Volume and Issue:
97(10), P. 5753 - 5761
Published: March 5, 2025
The
global
surge
in
diabetes
mellitus
(DM)
and
its
associated
complications
has
prompted
significant
efforts
to
mitigate
this
growing
public
health
challenge.
Among
these
complications,
diabetic
nephropathy
(DN)
is
of
particular
concern
due
high
rates
morbidity
mortality.
Extensive
research
identified
methylglyoxal
(MGO)
MGO-derived
advanced
glycation
end
products
(AGEs)
as
critical
contributors
the
pathogenesis
DN.
Thus,
there
a
pressing
need
for
precise
effective
methodologies
monitoring
MGO
levels
complicated
biological
systems.
Herein,
we
report
first
Eu3+
complex-based
dual-mode
luminescence
probe,
DAPTTA-Eu3+,
accurate
detection
using
time-gated
(TGL)
lifetime
measurements.
probe
initially
exists
"dark
state"
characterized
by
relatively
short
lifetime.
Upon
interaction
with
MGO,
intense
emission
restored,
accompanied
increase
These
features
enable
DAPTTA-Eu3+
serve
reliable
quantification,
utilizing
TGL
complementary
strategies.
Moreover,
cell
membrane-permeable
derivative
AM-DAPTTA-Eu3+,
was
prepared
used
imaging
both
exogenous
endogenous
live
cells,
which
also
allowed
fluctuations
kidneys
DN
mice
nephroprotective
effects
metformin
against
be
assessed.
Notably,
exploiting
differential
expressions
renal
cisplatin-induced
acute
kidney
injury
(AKI)
were
successfully
distinguished.
results
underscored
practicability
AM-DAPTTA-Eu3+
across
varying
kidney-related
pathophysiological
conditions,
suggesting
potential
clinical
diagnosis.
Journal of the American Chemical Society,
Journal Year:
2025,
Volume and Issue:
unknown
Published: May 29, 2025
Sulfur
dioxide
(SO2),
as
an
endogenous
gasotransmitter,
is
involved
in
a
variety
of
physiological
processes
living
systems,
however,
distinction
and
quantification
base-level
SO2
live
cells
pose
great
challenges
for
the
current
analytical
techniques.
We
report
efficient
way
to
visualize
quantify
different
mammalian
by
19F-NMR.
This
method
relies
on
high-performance
19F-probe
(P1-CF3)
that
reacts
with
reactive
sulfur
species
(RSS)
generation
distinct
chemical
shift
profiles,
which
are
well-resolved
NMR
spectrum.
The
quantitative
reaction
fast
produces
stable
addition
product
well
distinguishable
between
intra-
extracellular
environment.
high
performance
this
discloses
varies
greatly
cell
lines.
accuracy
precision
RSS
measurement
19F
probe
allow
simultaneous
culture
medium
real
time
without
interference
from
other
potential
competitors
also
background
signals.
ChemBioChem,
Journal Year:
2024,
Volume and Issue:
unknown
Published: July 23, 2024
Abstract
Cyanine‐based
near‐infrared
(NIR)
fluorescent
probes
have
played
vital
roles
in
biological
application
due
to
their
low
interference
from
background
fluorescence,
deep
tissue
penetration,
high
sensitivity,
and
minimal
photodamage
samples.
They
are
widely
utilized
molecular
recognition,
medical
diagnosis,
biomolecular
detection,
imaging.
Herein,
we
provide
a
review
of
recent
advancements
cyanine‐based
NIR
for
the
detection
pH,
cells,
tumor
as
well
photothermal
therapy
(PTT)
photodynamic
(PDT).
Chemistry - A European Journal,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Dec. 10, 2024
Abstract
The
development
of
redox‐sensitive
molecular
fluorescent
probes
for
the
detection
redox
changes
in
Plasmodium
falciparum
‐parasitized
red
blood
cells
remains
interest
due
to
limitations
current
genetically
encoded
biosensors.
This
study
describes
design,
screening
and
synthesis
new
pro‐fluorophores
based
on
flavylium
azido
dyes
coupled
by
CuAAC
click
chemistry
alkynyl
analogues
plasmodione
oxide,
key
metabolite
potent
redox‐active
antimalarial
plasmodione.
photophysical
electrochemical
properties
these
were
evaluated,
focusing
their
fluorogenic
responses.
influence
both
status
quinone
length
PEG
chain
separating
fluorophore
from
electrophore
was
investigated.
fluorescence
quenching
photoinduced
electron
transfer
is
reversible
high
amplitude
oxidized
forms
reinstated
reduced
hydroquinone
forms.
Our
results
demonstrate
that
shortening
has
effect
enhancing
response,
likely
non‐covalent
interactions
between
two
chromophores.
All
systems
evaluated
antiparasitic
activities
imaging
suggests
efficacy
P.
cells,
paving
way
future
parasite
studies
monitor
cellular
processes.
