International Journal of Molecular Sciences,
Journal Year:
2024,
Volume and Issue:
25(12), P. 6699 - 6699
Published: June 18, 2024
Despite
the
recognized
potential
of
nanoparticles,
only
a
few
formulations
have
progressed
to
clinical
trials,
and
an
even
smaller
number
been
approved
by
regulatory
authorities
marketed.
Virus-like
particles
(VLPs)
emerged
as
promising
alternatives
conventional
nanoparticles
due
their
safety,
biocompatibility,
immunogenicity,
structural
stability,
scalability,
versatility.
Furthermore,
VLPs
can
be
surface-functionalized
with
small
molecules
improve
circulation
half-life
target
specificity.
Through
functionalization
coating
VLPs,
it
is
possible
optimize
response
properties
given
stimulus,
such
heat,
pH,
alternating
magnetic
field,
or
enzymes.
Surface
also
modulate
other
properties,
specificity,
deeming
vaccine
candidates
delivery
systems.
This
review
aims
address
different
types
surface
highlighting
more
recent
cutting-edge
technologies
that
explored
for
design
tailored
importance,
consequent
applicability
in
medical
field.
Analytical Chemistry,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 11, 2025
Ochratoxin
A
(OTA)
is
a
type
of
mycotoxin
found
in
various
contaminated
foods,
and
it
highly
toxic
to
the
livers
kidneys
humans.
Herein,
we
develop
self-enhanced
electrochemiluminescent
(ECL)
sensor
based
on
tandem
signal
amplification
self-luminescent
europium
covalent-organic
polymer
(Eu-COPTMT-BPA)
for
OTA
assay.
Eu-COPTMT-BPA
ECL
emitter
that
obtained
by
using
2,4,6-trimethyl-1,3,5-triazine
(TMT)
2,2′-bipyridine-5,5′-dicarbaldehyde
(BPA)
as
first
second
ligands
form
conjugated
structure
sensitizing
Eu3+
luminescence.
In
Eu-COPTMT-BPA,
BPA
can
adjust
energy
gap
between
triplet
ligand
Eu3+excited
state
achieve
rapid
effective
transfer
generation
an
enhanced
signal.
When
target
present,
binds
with
aptamer
segment
hairpin
probe
(HAP)
tight
structure.
The
exposed
sequence
HAP
subsequently
1/single-stranded
DNA
1
(HP1/S1)
hybrid
release
trigger
(S1).
released
S1
be
recognized
magnetic
bead
(MB)-capture
conjugate
hybridization
chain
reaction
(HCR)
Fc-labeled
HP2
HP3,
leading
formation
long
double-stranded
(dsDNA)
nanowires
MB
surface
accumulation
abundant
Fc
quench
intensity
Eu-COPTMT-BPA/TPrA
system.
This
exhibits
good
stability,
excellent
specificity,
high
sensitivity,
detection
limit
(LOD)
0.47
fg/mL
linear
range
pg/mL–10
ng/mL,
quantitatively
measure
wine
coffee.
Precision Chemistry,
Journal Year:
2025,
Volume and Issue:
3(4), P. 187 - 205
Published: March 3, 2025
Nonenzymatic
nucleic
acid
amplification
reactions,
especially
nonenzymatic
DNA
reactions
(NDARs),
are
thermodynamically
driven
processes
that
operate
without
enzymes,
relying
on
toehold-mediated
strand
displacement
(TMSD)
and
branch
migration.
With
their
sensitive
efficient
signal
capabilities,
NDARs
have
become
essential
tools
for
biomarker
detection
intracellular
imaging.
They
encompass
four
primary
methods:
catalytic
hairpin
assembly
(CHA),
hybridization
chain
reaction
(HCR),
DNAzyme-based
amplification,
entropy-driven
circuits
(EDC).
Based
mechanisms,
can
be
categorized
into
three
types:
stimuli-responsive
NDARs,
which
employ
single
strategies
triggered
by
specific
stimuli
like
pH,
light,
or
biomolecules;
cascade
integrate
two
more
stepwise
enhancement;
autocatalytic
achieve
exponential
through
self-sustained
cycling.
These
advanced
designs
progressively
improve
efficiency,
enhance
sensitivity,
minimize
background
noise,
enabling
precise
of
proteins,
viruses,
acids
as
well
applications
in
cancer
cell
imaging
therapy.
Compared
with
classical
these
approaches
significantly
reduce
leakage,
offering
broader
applicability
diagnostics,
imaging,
therapeutic
contexts.
This
review
summarizes
recent
advancements,
addresses
existing
challenges,
explores
future
directions,
providing
insights
the
development
NDARs.
Advanced Functional Materials,
Journal Year:
2025,
Volume and Issue:
unknown
Published: April 11, 2025
Abstract
The
precise
detection
of
multiple
microRNAs
(miRNAs)
is
great
significance
to
the
classification
various
cell
subtypes.
However,
miRNA
hindered
by
signal
leakage,
false‐positive
signals
and
low
expression
miRNAs.
Herein,
a
DNA
nanodevice
in
second
near
infrared
region
(NIR‐II,
1000‐1700
nm)
(DND‐II)
developed
for
dual
miRNAs
bioimaging
based
on
“AND”
logic‐gate
amplification
strategy.
spatiotemporally
controlled
logic‐gate‐based
DND‐II
fabricated
thermosensitive
liposomes
integrated
with
near‐infrared
probes.
In
particular,
“always‐on”
photoacoustic
probes
provided
an
additional
imaging
tool
monitor
distribution
vivo.
When
both
are
present,
generated
“turn
on”
fluorescent
through
logical
analysis
molecular
computation,
further
triggering
entropy‐driven
dual‐cycle
fluorescence
amplification.
spatiotemporal
provides
powerful
platform
early
diagnosis
photothermal
ablation
different
subtypes
tumor
cells.
