Developing a polarity-specialized TICT fluorescent probe for wash-free and long-term monitoring lipid droplets dynamics

Talanta, Journal Year: 2025, Volume and Issue: 294, P. 128191 - 128191

Published: April 19, 2025

Language: Английский

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Trends in small-molecule probes for lipid droplets

TrAC Trends in Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 118287 - 118287

Published: May 1, 2025

Language: Английский

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Fluorescent probes for lysosomes, mitochondria, and lipid droplets: precision design, dynamic microenvironment monitoring, and heterogeneity exploration

Chemical Communications, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

This feature article highlights cutting-edge fluorescent probes for monitoring dynamic microenvironments and exploring heterogeneity in lysosomes, mitochondria, lipid droplets.

Language: Английский

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Rational design of an AIEgen for imaging lipid droplets polarity change during ferroptosis

Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy, Journal Year: 2024, Volume and Issue: 321, P. 124701 - 124701

Published: June 19, 2024

Language: Английский

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3D Imaging of Lipid Droplet–Nuclear Membrane Contact Sites and Cirrhotic Lipid Droplet Overexpression

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(31), P. 12908 - 12915

Published: July 27, 2024

To coordinate cellular physiology, cells rely on the rapid exchange of molecules at specialized organelle–organelle contact sites. Lipid droplets (LDs) and nuclear membrane (NM) sites are particularly vital communication hubs, playing key roles in signaling molecules, lipids, metabolites. However, there is still a lack understanding specific morphology Here, we combine advanced three-dimensional (3D) imaging with high-brightness fluorescent probe specifically targeting LDs to map structural landscape LD–NM The exhibits exceptional photophysical properties, making it highly suitable for visualizing changes occurring during apoptosis process. In addition, utilize advantages accurately monitor overexpression abnormal cirrhosis by 3D first time. outcomes this investigation highlight that has potential as robust tool investigate intricate biological functions their implications related diseases.

Language: Английский

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Washing-free fluorescent probes for visualizing the process of lipophagy and identifying non-alcoholic fatty liver disease

Microchemical Journal, Journal Year: 2024, Volume and Issue: unknown, P. 112603 - 112603

Published: Dec. 1, 2024

Language: Английский

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Recent advances in single fluorescent probes for monitoring dual organelles in two channels

Smart Molecules, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 15, 2024

Abstract Organelles are specialized areas where cells perform specific processes necessary for life and actively communicate with each other to keep the whole cell functioning. Disorders of organelle networks associated multiple pathological processes. However, clearly intuitively visualizing highly dynamic interactions between ultrafine organelles is challenging. Fluorescence imaging technology provides opportunities due distinct advantages facile, non‐invasiveness detection, making it particularly well‐suited applications in uncovering mysterious veil interactions. Regrettably, lack ideal fluorescence agents has always been an obstacle intricate behaviors organelles. In this review, we provide a systematic discussion on existing dual‐color dual‐targetable molecular sensors used monitoring interactions, focus their targeting strategies, mechanisms biological applications. Additionally, current limitations future development directions probes dual‐emissives briefly discussed. This review aims guidance researchers develop more improved studying biomedical field.

Language: Английский

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Recent progresses in organelle-targeting fluorescent probes for the detection of peroxynitrite

Chemical Communications, Journal Year: 2024, Volume and Issue: 60(93), P. 13629 - 13640

Published: Jan. 1, 2024

This review describes the updates and advances of ONOO − fluorescent probes from unique perspective organelle-targeting. The related design strategy, spectral responses, response mechanisms applications are thoroughly discussed.

Language: Английский

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Targeted Conversion from Mitochondria to the Nucleus of Hydroxystyrylpyridinium by Introducing Only an Additional o-Hydroxyl Group

Analytical Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 3, 2024

Aromatic cationic groups serve as crucial building blocks for the design of fluorescent probes targeting both nucleus and mitochondria. Therefore, it is a significant challenge to develop aromatic cation-based that accurately image without interference from However, this also presents an opportunity rational by modifying originally mitochondria redirect their toward nucleus. This study showcases rapid development novel nucleus-targeting probe (DHSP) through targeted conversion strategy based on structure modification hydroxystyrylpyridinium (HSP), well-established two-photon targets Importantly, DHSP, which derived exclusively introducing only additional o-hydroxyl group into HSP, exhibits robust DNA-binding capability comparable commercially available nuclear dye 4′,6-diamidino-2-phenylindole (DAPI). As result, rapidly enters within 5 min finds successful application in cellular intravital imaging

Language: Английский

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Tailoring the Polarity Sensitivity of Fluorescence Lifetime Imaging Probes to Reveal Interplays of Mitochondria Dysfunction with Lipid Droplets Formation

Sensors and Actuators B Chemical, Journal Year: 2024, Volume and Issue: unknown, P. 137027 - 137027

Published: Dec. 1, 2024

Language: Английский

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