Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
96(13), P. 5134 - 5142
Published: March 20, 2024
Mitochondria
are
important
organelles
that
provide
energy
for
cellular
physiological
activities.
Changes
in
their
structures
may
indicate
the
occurrence
of
diseases,
and
super-resolution
imaging
mitochondria
is
great
significance.
However,
developing
fluorescent
probes
mitochondrial
visualization
still
remains
challenging
due
to
insufficient
fluorescence
brightness
poor
stability.
Herein,
we
rationally
synthesized
an
ultrabright
xanthene
probe
Me-hNR
mitochondria-specific
using
structured
illumination
microscopy
(SIM).
The
rigid
structure
provided
its
ultrahigh
quantum
yield
up
0.92
16,000.
Occupying
para-position
O
atom
skeleton
by
utilizing
smallest
methyl
group
ensured
excellent
study
photophysical
process
indicated
mainly
emitted
via
radiative
decay,
nonradiative
decay
inter-system
crossing
were
rare
slow
rate
large
gap
(ΔEst
=
0.55
eV).
Owing
these
merits,
can
specifically
light
at
ultralow
concentrations
down
5
nM.
unprecedented
spatial
resolution
with
fwhm
174
nm
was
also
achieved.
Therefore,
this
has
potential
visualizing
structural
changes
revealing
pathogenesis
related
diseases
SIM.
Advanced Materials,
Journal Year:
2024,
Volume and Issue:
36(31)
Published: May 21, 2024
Fluorescence
imaging
(FLI)-guided
phototheranostics
using
emission
from
the
second
near-infrared
(NIR-II)
window
show
significant
potential
for
cancer
diagnosis
and
treatment.
Clinical
imaging-used
polymethine
ionic
indocyanine
green
(ICG)
dye
is
widely
adopted
NIR
fluorescence
imaging-guided
photothermal
therapy
(PTT)
research
due
to
its
exceptional
photophysical
properties.
However,
ICG
has
limitations
such
as
poor
photostability,
low
conversion
efficiency
(PCE),
short-wavelength
peak,
liver-targeting
issues,
which
restrict
wider
use.
In
this
study,
two
derivatives
are
transformed
into
neutral
merocyanines
(mCy)
achieve
much-enhanced
performance
NIR-II
phototheranostics.
Initial
designs
of
dyes
similar
drawbacks
in
terms
photostability
performance.
One
modified
molecules,
mCy890,
shows
significantly
improved
stability,
an
peak
over
1000
nm,
a
high
PCE
51%,
all
considerably
outperform
ICG.
vivo
studies
demonstrate
that
nanoparticles
mCy890
can
effectively
accumulate
at
tumor
sites
guided
by
imaging.
This
provides
valuable
insights
development
enhanced
Sensors and Actuators B Chemical,
Journal Year:
2024,
Volume and Issue:
409, P. 135496 - 135496
Published: Feb. 15, 2024
A
fluorescent
probe
(probe
1)
with
selectivity
and
sensitivity
for
H2S
N2H4
has
been
developed.
Probe
1
exhibits
dual-excitation
dual-emission
fluorescence
response
to
enhancement
toward
distinct
naked-eye
color
changes.
Moreover,
displayed
low
detection
limits
of
0.90
nM
23.6
nM,
respectively.
The
reaction
mechanism
was
established
using
1H
NMR
HR-MS
spectroscopy.
Additionally,
used
monitoring
differentiating
in
HeLa
cells
displays
good
biocompatibility
cytotoxicity.
Chemical & Biomedical Imaging,
Journal Year:
2023,
Volume and Issue:
1(6), P. 509 - 521
Published: July 3, 2023
Observations
of
the
micro
world,
especially
structures
organelles,
have
been
attractive
topics
since
17th
century.
As
a
powerful
detection
tool,
fluorescence
technique
has
played
significant
role
in
bioimaging
to
provide
more
details
and
enhance
signal-to-noise
ratio
compared
that
traditional
optical
microscopes.
The
boom
aggregate-induced
emission
luminogens
(AIEgens)
last
two
decades
revolutionized
design
strategy
luminescent
materials
for
biological
applications.
This
Review
summarizes
advantages
recent
progress
AIEgens
imaging
tracking.
Different
strategies
including
turn-on
imaging,
stimuli-response
sensing,
long-term
tracking
are
presented.
NIR
used
in-depth
via
different
methods
also
discussed.
Finally,
we
propose
several
potential
development
directions
bioimaging.
Chemical Communications,
Journal Year:
2024,
Volume and Issue:
60(22), P. 2994 - 3007
Published: Jan. 1, 2024
Mitochondria,
as
essential
organelles
in
cells,
play
a
crucial
role
cellular
growth
and
apoptosis.
Monitoring
mitochondria
is
of
great
importance,
mitochondrial
dysfunction
often
considered
hallmark
event
cell
Traditional
fluorescence
probes
used
for
imaging
sensing
are
mostly
intensity-based
susceptible
to
factors
such
concentration,
the
probe
environment,
intensity.
Probes
based
on
resonance
energy
transfer
(FRET)
can
effectively
overcome
external
interference
achieve
high-contrast
well
quantitative
monitoring
microenvironments.
This
review
focuses
recent
advances
application
FRET-based
structure
microenvironment
sensing.
