In
anaerobic
digestion
(AD)
research,
optimizing
methane
production
from
waste
activated
sludge
(WAS)
is
a
core
focus.
This
study
investigated
the
efficacy
and
mechanism
of
deep
eutectic
solvents
(DES)
as
pretreatment
agents
for
AD
systems
WAS.
Two
DESs
were
developed,
key
findings
included
significant
increases
in
production:
390%
choline
chloride-urea
(ChCl-Urea)
540%
chloride-ethylene
glycol
(ChCl-EG).
Results
showed
that
ChCl-Urea
mainly
disrupted
extracellular
polymeric
substances
(EPS)
structures,
aiding
initial
solubilization
during
pretreatment.
ChCl-EG,
instead,
induced
self-driven
organic
enhanced
hydrolysis
acidification
process.
Based
on
extent
to
which
two
promoted
production,
process
can
be
divided
into
stage
Ⅰ
Ⅱ.
Ⅰ,
ChCl-EG
methanogenesis
more
significantly.
this
stage,
both
enriched
acetotrophic
methanogens-Methanosarcina,
thereby
promoting
acetoclastic
methanogenesis.
Notably,
particularly
influenced
polysaccharide-related
metabolism,
whereas
targeted
protein-related
metabolism.
Ⅱ,
promotion
role
was
dominant.
bolstered
metabolism
by
methanogenic
archaea,
directly
boosting
Conversely,
genetic
information
processing
acidogenic
bacteria,
indirectly
amplifying
essence,
microbial
DES-enhanced
provided
reference
future
research.
Fermentation,
Journal Year:
2024,
Volume and Issue:
10(7), P. 370 - 370
Published: July 19, 2024
At
psychrophilic
temperatures
(<20
°C),
anaerobic
digestion
produces
less
methane
(CH4).
For
(PAD)
to
be
successful,
investigation
of
cold-adapted
microbial
consortia
involved
in
production
is
critical.
This
study
aimed
investigate
the
community
driving
enhanced
from
cold-adaptation
process
and
bioaugmentation
PAD
with
inoculum
(BI).
Microbial
cattle
manure
(CM)
food
waste
(FW)
were
adapted
applied
during
batch
CM
FW
bioaugment
at
15
°C.
Cold
adaptation
BI
resulted
cumulative
specific
yields
0.874
±
0.231
0.552
0.089
L
CH4
g−1
volatile
solids,
respectively,
after
14
weeks,
while
absence
(control)
led
acidification
no
PAD.
Following
16S
rRNA
V4–V5
amplicon
sequencing
metagenomic
analyses,
Methanosarcina
was
revealed
as
a
key
driver
methanogenesis
cold
bioaugmentation.
Furthermore,
based
on
predictive
functional
metabolic
analysis
communities,
possible
synergies
proposed
terms
substrate
utilization
by
dominant
groups.
instance,
production,
Bacteroides
Methanobrevibacter
possibly
syntrophic
relationship,
which
promoted
Methanosarcina.
These
findings
provide
insight
into
prospective
that
can
harnessed
and/or
regulated
for
improvement
