A novel electrochemiluminescent cytosensor using dual-target magnetic probe recognition and nanozymes-catalyzed cascade signal amplification for precise phenotypic enumeration of CTCs DOI Creative Commons
Congcong Shen, Simin Fan, Xiaoqing Li

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 11, 2024

Abstract The inability of surgical biopsy to monitor the dynamic evolution cancer cells hampers its capacity reflect real-time tumor heterogeneity. Circulating (CTCs), as a crucial target in liquid biopsy, offer novel approach for accurate monitoring tumors. However, rarity and complex phenotype resulting from epithelial mesenchymal transition pose challenges conventional methods such CellSearch immunohistochemistry, which have insufficient ability simultaneous phenotyping enumeration CTCs. single CTCs is accurately assessing disease progression. Herein, we propose strategy address this issue by fabricating an electrochemiluminescence cytosensor via integration dual-target enrichment nanozymes-catalyzed cascade signal amplification. graphene oxide@hollow mesoporous prussian blue/Pt (GO@HMPB/Pt) complex, possessing large specific surface area exceptional catalytic activity, employed loading substantial amount luminol probe. Dual-target magnetic PPy@Fe3O4/Au-antibody/aptamer utilized capture both interstitial Glutathione (GSH) can disrupt Au-S bond on aptamer thiol exchange reaction selectively release subset phenotypic CTCs, thereby facilitating efficient capture, classification, ultrasensitive detection peripheral blood. Using MCF-7 Hela models, ECL demonstrates excellent performance identifying spiked into whole This study presents early metastasis, tracking recurrence, therapeutic efficacy.

Language: Английский

Metal–organic framework-engineered enzyme/nanozyme composites: Preparation, functionality, and sensing mechanisms DOI
Yujie Li, Huining Chai,

Zhishuang Yuan

et al.

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 496, P. 153884 - 153884

Published: July 9, 2024

Language: Английский

Citations

25
Engineering metal-organic frameworks-based nanozymes for enhanced biomimetic catalytic sensing DOI
Zhong Wei Jiang, Xue Gong, Yi Wang

et al.

TrAC Trends in Analytical Chemistry, Journal Year: 2024, Volume and Issue: 178, P. 117862 - 117862

Published: July 10, 2024

Language: Английский

Citations

19
Nanozyme‐Enabled Biomedical Diagnosis: Advances, Trends, and Challenges DOI Creative Commons

Qingzhen Tian,

Shu Li, Zheng Tang

et al.

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 13, 2024

Abstract As nanoscale materials with the function of catalyzing substrates through enzymatic kinetics, nanozymes are regarded as potential alternatives to natural enzymes. Compared protein‐based enzymes, exhibit attractive characteristics low preparation cost, robust activity, flexible performance adjustment, and versatile functionalization. These advantages endow them wide use from biochemical sensing environmental remediation medical theranostics. Especially in biomedical diagnosis, feature catalytic signal amplification provided by makes emerging labels for detection biomarkers diseases, rapid developments observed recent years. To provide a comprehensive overview progress made this dynamic field, here an diagnosis enabled is provided. This review first summarizes synthesis nanozyme then discusses main strategies applied enhance their activity specificity. Subsequently, representative utilization combined biological elements disease reviewed, including related metabolic, cardiovascular, nervous, digestive diseases well cancers. Finally, some development trends nanozyme‐enabled highlighted, corresponding challenges also pointed out, aiming inspire future efforts further advance promising field.

Language: Английский

Citations

18
Understanding the Electrochemical MOF Sensors in Detecting Cancer with Special Emphasis on Breast Carcinoma Biomarkers DOI
Brij Mohan, Krunal Modi, Gurjaspreet Singh

et al.

Topics in Current Chemistry, Journal Year: 2025, Volume and Issue: 383(1)

Published: Feb. 18, 2025

Language: Английский

Citations

3
Nanozyme-based aptasensors for the detection of tumor biomarkers DOI Creative Commons
Ke Wang,

Xiaogang Lin,

Xiao Lv

et al.

Journal of Biological Engineering, Journal Year: 2025, Volume and Issue: 19(1)

Published: Feb. 7, 2025

Language: Английский

Citations

2
A novel electrochemiluminescent cytosensor using 3D multivalent aptamer recognition and covalent organic frameworks-associated DNA walker for highly efficient capture and detection of rare CTCs DOI
Minghui Guo, Bo Shen, Wen He

et al.

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 481, P. 148606 - 148606

Published: Jan. 6, 2024

Language: Английский

Citations

14
Ultrasensitive detection of H2O2 via electrochemical sensor by graphene synergized with MOF-on-MOF nanozymes DOI
Haifeng Gao,

Haiting Yu,

Shuang Yang

et al.

Microchimica Acta, Journal Year: 2024, Volume and Issue: 191(8)

Published: July 24, 2024

Language: Английский

Citations

12
A dual action electrochemical molecular imprinting sensor based on FeCu-MOF and RGO/PDA@MXene hybrid synergies for trace detection of ribavirin DOI
Haifeng Gao,

Junyi You,

Hongbo Wu

et al.

Food Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 143092 - 143092

Published: Jan. 1, 2025

Language: Английский

Citations

1
Aptamer-based electrochemical analysis platform for tumor cells and biomarkers detection DOI
Yihan Wang,

Wenyan Yao,

Wenyu Sun

et al.

Journal of Electroanalytical Chemistry, Journal Year: 2024, Volume and Issue: 960, P. 118194 - 118194

Published: March 15, 2024

Language: Английский

Citations

7
Electrochemical Monitoring of Sphingosine-1-phosphate-Induced ATP Release Using a Microsensor Based on an Entropy-Driven Bipedal DNA Walker DOI
Hong Jiang, Xiao Liu,

Yu-Kang Jia

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(14), P. 5719 - 5726

Published: March 28, 2024

Neuropathic pain is a chronic and severe syndrome for which effective therapy insufficient the release of ATP from microglia induced by sphingosine-1-phosphate (S1P) plays vital role in neuropathic pain. Therefore, there an urgent demand to develop highly sensitive selective biosensors quantitative monitoring low-concentration complex nervous system, helps understanding mechanism involved Herein, we developed electrochemical microsensor based on entropy-driven bipedal DNA walker. First, specifically recognized via aptamers, initiating Subsequently, walker autonomously traversed microelectrode interface, introducing methylene blue electrode surface achieving cascade signal amplification. This showed excellent selectivity, stability, low limit detection at 1.13 nM. The S1P-induced BV2 cells was successfully monitored, it observed that dicumarol could inhibit this release, suggesting as potential treatment microsensor's small size exhibited significant level changes vivo, provides new strategy situ nonelectroactive biomolecules associated with neurological diseases.

Language: Английский

Citations

6