The molecular basis for cellular function of intrinsically disordered protein regions

Nature Reviews Molecular Cell Biology, Journal Year: 2023, Volume and Issue: 25(3), P. 187 - 211

Published: Nov. 13, 2023

Language: Английский

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Increasing the resilience of plant immunity to a warming climate

Nature, Journal Year: 2022, Volume and Issue: 607(7918), P. 339 - 344

Published: June 29, 2022

Abstract Extreme weather conditions associated with climate change affect many aspects of plant and animal life, including the response to infectious diseases. Production salicylic acid (SA), a central defence hormone 1–3 , is particularly vulnerable suppression by short periods hot above normal growth temperature range via an unknown mechanism 4–7 . Here we show that SA production in Arabidopsis thaliana at 28 °C independent PHYTOCHROME B 8,9 (phyB) EARLY FLOWERING 3 10 (ELF3), which regulate thermo-responsive development. Instead, found formation GUANYLATE BINDING PROTEIN-LIKE (GBPL3) defence-activated biomolecular condensates 11 (GDACs) was reduced higher temperature. The altered GDAC vivo linked impaired recruitment GBPL3 SA-associated Mediator subunits promoters CBP60g SARD1 encode master immune transcription factors. Unlike other signalling components, receptor biosynthetic genes, optimized expression sufficient broadly restore production, basal immunity effector-triggered elevated without significant trade-offs. family factors are widely conserved plants 12 These results have implications for safeguarding system as well understanding concept plant–pathogen–environment disease triangle emergence new epidemics warming climate.

Language: Английский

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The material properties of a bacterial-derived biomolecular condensate tune biological function in natural and synthetic systems

Nature Communications, Journal Year: 2022, Volume and Issue: 13(1)

Published: Sept. 26, 2022

Intracellular phase separation is emerging as a universal principle for organizing biochemical reactions in time and space. It remains incompletely resolved how biological function encoded these assemblies whether this depends on their material state. The conserved intrinsically disordered protein PopZ forms condensates at the poles of bacterium Caulobacter crescentus, which turn orchestrate cell-cycle regulating signaling cascades. Here we show that properties are determined by balance between attractive repulsive forces mediated helical oligomerization domain an expanded region, respectively. A series mutants disrupting results span spectrum, from liquid to solid. narrow range condensate supports proper cell division, linking emergent organismal fitness. We use insights repurpose modular platform generating tunable synthetic human cells.

Language: Английский

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Intrinsically disordered regions are poised to act as sensors of cellular chemistry

Trends in Biochemical Sciences, Journal Year: 2023, Volume and Issue: 48(12), P. 1019 - 1034

Published: Aug. 31, 2023

Intrinsically disordered proteins and protein regions (IDRs) are abundant in eukaryotic proteomes play a wide variety of essential roles. Instead folding into stable structure, IDRs exist an ensemble interconverting conformations whose structure is biased by sequence-dependent interactions. The absence 3D combined with high solvent accessibility, means that IDR conformational biases inherently sensitive to changes their environment. Here, we argue ideally poised act as sensors actuators cellular physicochemistry. We review the physical principles underlie sensitivity, molecular mechanisms translate this sensitivity function, recent studies where environmental sensing may key role downstream function.

Language: Английский

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Regulatory networks in plant responses to drought and cold stress

PLANT PHYSIOLOGY, Journal Year: 2024, Volume and Issue: 195(1), P. 170 - 189

Published: March 21, 2024

Abstract Drought and cold represent distinct types of abiotic stress, each initiating unique primary signaling pathways in response to dehydration temperature changes, respectively. However, a convergence at the gene regulatory level is observed where common set stress-responsive genes activated mitigate impacts both stresses. In this review, we explore these intricate networks, illustrating how plants coordinate stress signals into collective transcriptional strategy. We delve molecular mechanisms perception, signaling, activation pathways, with focus on insights gained from model species. By elucidating shared aspects plant responses drought cold, provide insight adaptive strategies plants, paving way for engineering stress-resilient crop varieties that can withstand changing climate.

Language: Английский

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Molecular mechanisms of adaptive evolution in wild animals and plants

Science China Life Sciences, Journal Year: 2023, Volume and Issue: 66(3), P. 453 - 495

Published: Jan. 13, 2023

Language: Английский

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Stress-related biomolecular condensates in plants

The Plant Cell, Journal Year: 2023, Volume and Issue: 35(9), P. 3187 - 3204

Published: May 10, 2023

Biomolecular condensates are membraneless organelle-like structures that can concentrate molecules and often form through liquid-liquid phase separation. condensate assembly is tightly regulated by developmental environmental cues. Although research on biomolecular has intensified in the past 10 years, our current understanding of molecular mechanisms components underlying their formation remains its infancy, especially plants. However, recent studies have shown may be central to plant acclimation stress conditions. Here, we describe mechanism, regulation, properties stress-related plants, focusing granules processing bodies, 2 most well-characterized condensates. In this regard, showcase proteomes bodies an attempt suggest methods for elucidating composition function Finally, discuss how modulate responses they might used as targets biotechnological efforts improve tolerance.

Language: Английский

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When Phased without Water: Biophysics of Cellular Desiccation, from Biomolecules to Condensates

Chemical Reviews, Journal Year: 2023, Volume and Issue: 123(14), P. 9010 - 9035

Published: May 3, 2023

The molecular machinery that enables life has evolved in water, yet many of the organisms around us are able to survive even extreme desiccation. Especially remarkable single-cell and sedentary rely on specialized biomolecular environments routinely subjected a near-complete lack water. In this review, we zoom level what is happening cellular environment under water stress. We cover various mechanisms by which biochemical components cell can dysfunction dehydrated cells detail different strategies have eliminate or cope with these desiccation-induced perturbations. specifically focus two survival strategies: (1) use disordered proteins protect before, during, recovery from desiccation, (2) condensates as self-assembly mechanism sequester specific times provide summary experimental work describing critical contributions response loss highlight their role desiccation tolerance. Desiccation biology an exciting area biology, still far being completely explored. Understanding it bound give new insights how adapted/can adapt spanning early colonization land deal climate change our future.

Language: Английский

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The temperature sensor TWA1 is required for thermotolerance in Arabidopsis

Nature, Journal Year: 2024, Volume and Issue: 629(8014), P. 1126 - 1132

Published: May 15, 2024

Plants exposed to incidences of excessive temperatures activate heat-stress responses cope with the physiological challenge and stimulate long-term acclimation

Language: Английский

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How plants sense and respond to osmotic stress

Journal of Integrative Plant Biology, Journal Year: 2024, Volume and Issue: 66(3), P. 394 - 423

Published: Feb. 8, 2024

ABSTRACT Drought is one of the most serious abiotic stresses to land plants. Plants sense and respond drought stress survive under water deficiency. Scientists have studied how plants stress, or osmotic caused by drought, ever since Charles Darwin, gradually obtained clues about sensing signaling in Osmotic a physical stimulus that triggers many physiological changes at cellular level, including turgor, cell wall stiffness integrity, membrane tension, fluid volume, may some these stimuli trigger downstream responses. In this review, we emphasized potential movements organisms, compared putative signal inputs wall‐containing wall‐free prospected volume according advances plants, animals, yeasts, bacteria, summarized multilevel biochemical outputs, such as plasma nanodomain formation, permeability, root hydrotropism, halotropism, Casparian strip suberin lamellae, finally proposed hypothesis responses are likely be cocktail mediated multiple osmosensors. We also discussed core scientific questions, provided perspective future directions field, highlighted importance robust smart systems efficient source‐sink allocations for generating high‐yield stress‐resistant crops

Language: Английский

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