Integrating organoids and organ-on-a-chip devices

Nature Reviews Bioengineering, Journal Year: 2024, Volume and Issue: 2(7), P. 588 - 608

Published: July 2, 2024

Language: Английский

---

Human organoids-on-chips for biomedical research and applications

Theranostics, Journal Year: 2024, Volume and Issue: 14(2), P. 788 - 818

Published: Jan. 1, 2024

Human organoids-on-chips (OrgOCs) are the synergism of human organoids (HOs) technology and microfluidic organs-on-chips (OOCs).OOCs can mimic extrinsic characteristics organs, such as environmental clues living tissue, while HOs more amenable to biological analysis genetic manipulation.By spatial cooperation, OrgOCs served 3D organotypic models allowing them recapitulate critical tissue-specific properties forecast responses outcomes.It represents a giant leap forward from regular 2D cell monolayers animal in improved ecological niche modeling.In recent years, have offered potential promises for clinical studies advanced preclinical-to-clinical translation medical industrial fields.In this review, we highlight cutting-edge achievements OrgOCs, introduce key features architectures, share revolutionary applications basic biology, disease modeling, preclinical assay precision medicine.Furthermore, discuss how combine wide range disciplines with accelerate translational applications, well challenges opportunities biomedical research applications.

Language: Английский

---

A molecular and cellular perspective on human brain evolution and tempo

Nature, Journal Year: 2024, Volume and Issue: 630(8017), P. 596 - 608

Published: June 19, 2024

Language: Английский

---

Clinical applications of human organoids

Nature Medicine, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 3, 2025

Language: Английский

---

Microglia-like Cells Promote Neuronal Functions in Cerebral Organoids

Cells, Journal Year: 2021, Volume and Issue: 11(1), P. 124 - 124

Published: Dec. 30, 2021

Human cerebral organoids, derived from induced pluripotent stem cells, offer a unique in vitro research window to the development of cortex. However, key player developing brain, microglia, do not natively emerge organoids. Here we show that erythromyeloid progenitors (EMPs), differentiated migrate and mature into microglia-like cells interact with synaptic material. Patch-clamp electrophysiological recordings population supported emergence more diversified neuronal phenotypes displaying repetitive firing action potentials, low-threshold spikes activity, while multielectrode array revealed spontaneous bursting activity increased power gamma-band oscillations upon pharmacological challenge NMDA. To conclude, within organoids promote network maturation recapitulate some aspects microglia-neuron co-development vivo, indicating could be useful biorealistic human platform for studying interactions.

Language: Английский

---

Modeling human telencephalic development and autism-associated SHANK3 deficiency using organoids generated from single neural rosettes

Nature Communications, Journal Year: 2022, Volume and Issue: 13(1)

Published: Oct. 6, 2022

Human telencephalon is an evolutionarily advanced brain structure associated with many uniquely human behaviors and disorders. However, cell lineages molecular pathways implicated in telencephalic development remain largely unknown. We produce organoids from stem cell-derived single neural rosettes investigate under normal pathological conditions. show that rosette-derived contain pallial subpallial progenitors, excitatory inhibitory neurons, as well macroglial periendothelial cells, exhibit predictable organization cytoarchitecture. comprehensively characterize the properties of neurons SNR-derived identify transcriptional programs specification a common pool NPs early development. also demonstrate hemizygous deletion autism- intellectual disability-associated gene SHANK3 intrinsic synaptic deficits impaired expression several clustered protocadherins. Collectively, this study validates reliable model for studying cortico-striatal identifies intrinsic, synaptic, protocadherin tissue hemizygosity.

Language: Английский

---

Human assembloids

Development, Journal Year: 2022, Volume and Issue: 149(20)

Published: Oct. 15, 2022

ABSTRACT Deconstructing and then reconstructing developmental processes ex vivo is crucial to understanding how organs assemble physiology can be disrupted in disease. Human 3D stem cell-derived systems, such as organoids, have facilitated this pursuit; however, they often do not capture inter-tissue or inter-lineage cellular interactions that give rise emergent tissue properties during development. Assembloids are self-organizing systems result from the integration of multiple organoids combination with missing cell types primary explants. Here, we outline concept assembloids present their applications for studying nervous system other tissues. We describe tools used probe manipulate delineate current challenges potential new approach interrogate development

Language: Английский

---

Sites of transcription initiation drive mRNA isoform selection

Cell, Journal Year: 2023, Volume and Issue: 186(11), P. 2438 - 2455.e22

Published: May 1, 2023

The generation of distinct messenger RNA isoforms through alternative processing modulates the expression and function genes, often in a cell-type-specific manner. Here, we assess regulatory relationships between transcription initiation, splicing, 3′ end site selection. Applying long-read sequencing to accurately represent even longest transcripts from end, quantify mRNA Drosophila tissues, including transcriptionally complex nervous system. We find that heads, as well human cerebral organoids, choice is globally influenced by initiation (TSS). "Dominant promoters," characterized specific epigenetic signatures p300/CBP binding, impose transcriptional constraint define splice polyadenylation variants. In vivo deletion or overexpression dominant promoters loss disrupted landscape. Our study demonstrates crucial impact TSS on regulation transcript diversity tissue identity.

Language: Английский

---

Current advances in primate genomics: novel approaches for understanding evolution and disease

Nature Reviews Genetics, Journal Year: 2023, Volume and Issue: 24(5), P. 314 - 331

Published: Jan. 4, 2023

Language: Английский

---

Brain organoid protocols and limitations

Frontiers in Cellular Neuroscience, Journal Year: 2024, Volume and Issue: 18

Published: March 20, 2024

Stem cell-derived organoid technology is a powerful tool that revolutionizes the field of biomedical research and extends scope our understanding human biology diseases. Brain organoids especially open an opportunity for brain modeling many neurological diseases, which have lagged due to inaccessibility samples lack similarity with other animal models. can be generated through various protocols mimic whole or region-specific. To provide overview technology, we summarize currently available list several factors consider before choosing protocols. We also outline limitations current challenges need solved in future investigation development pathobiology.

Language: Английский

---