Acetate enables metabolic fitness and cognitive performance during sleep disruption

Cell Metabolism, Journal Year: 2024, Volume and Issue: 36(9), P. 1998 - 2014.e15

Published: Aug. 19, 2024

Language: Английский

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Activity Monitoring for Analysis of Sleep inDrosophila melanogaster

Cold Spring Harbor Protocols, Journal Year: 2024, Volume and Issue: 2024(11), P. pdb.top108095 - pdb.top108095

Published: Feb. 9, 2024

Sleep is important for survival, and the need sleep conserved across species. In past two decades, fruit fly

Language: Английский

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Protective effects of a probiotic-fermented germinated grain complex on neurotransmitters and sleep quality in sleep-deprived mice

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: July 29, 2024

To explore the effects of probiotic fermentation products germinated grains on cognitive and sleep improvement in mice with deprivation induced by chlorophenylalanine (PCPA), to provide theoretical experimental basis for development natural alleviate insomnia.

Language: Английский

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The Drosophila blood–brain barrier regulates sleep via Moody G protein-coupled receptor signaling

Proceedings of the National Academy of Sciences, Journal Year: 2023, Volume and Issue: 120(42)

Published: Oct. 13, 2023

Sleep is vital for most animals, yet its mechanism and function remain unclear. We found that permeability of the BBB (blood–brain barrier)—the organ required maintenance homeostatic levels nutrients, ions, other molecules in brain—is modulated by sleep deprivation (SD) can cell-autonomously effect changes. observed increased known mutants as well acutely sleep-deprived animals. In addition to molecular tracers, SD–induced changes also penetration drugs used treatment brain pathologies. After chronic/genetic or acute SD, rebound administration sleeping aid gaboxadol normalized permeability, showing SD effects on are reversible. Along with RNA master regulator moody sleep. Conversely, altering alone through glia-specific modulation moody, gαo, loco, lachesin , neuroglian —each a well-studied function—was sufficient induce robust phenotypes. These studies demonstrate tight link between indicate unique role regulation

Language: Английский

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Comparison of the Protective Effects of Casein Hydrolysate Containing Tyr-Pro-Val-Glu-Pro-Phe and Casein on the Behaviors and Peripheral and Brain Functions in Mice with Chronic-Stress-Induced Anxiety and Insomnia

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(20), P. 11515 - 11530

Published: May 10, 2024

Chronic stress is a major inducer of anxiety and insomnia. Milk casein has been studied for its stress-relieving effects. We previously prepared hydrolysate (CP) rich in the sleep-enhancing peptide YPVEPF, this study aims to systemically investigate different protective effects CP on dysfunction anxiety/insomnia behavior underlying mechanisms chronically stressed mice. Behavioral results showed that ameliorated stress-induced insomnia more effectively than milk casein, difference amelioration was highly correlated with an increase GABA, 5-HT, GABAA, 5-HT1A receptors, BDNF decrease IL-6 NMDA receptors Furthermore, restored these dysfunctions brain colon by activating HPA response, modulating ERK/CREB-BDNF-TrκB signaling pathway, alleviating inflammation. The abundant YPVEPF (1.20 ± 0.04%) Tyr-based/Trp-containing peptides may be key reasons compared casein. Thus, work revealed main active structures provided novel dietary intervention strategy prevention treatment chronic-stress-induced behaviors.

Language: Английский

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From sensory organs to internal pathways: A comprehensive review of amino acid sensing in Drosophila

Comparative Biochemistry and Physiology Part A Molecular & Integrative Physiology, Journal Year: 2025, Volume and Issue: unknown, P. 111828 - 111828

Published: Feb. 1, 2025

Language: Английский

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A gut hormone governing protein appetite and longevity

Nature Metabolism, Journal Year: 2025, Volume and Issue: unknown

Published: April 14, 2025

Language: Английский

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A Newly Evolved Small Secretory Peptide Enhances Mechanical Properties of Spider Silk

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: April 18, 2025

The ability of spiders to spin foraging webs by silk from specialized glands represents a remarkable evolutionary innovation, yet the molecular evolution remains poorly understood. Herein, we explored in Araneoidea through comprehensive genomic and transcriptomic mining. Following divergence ancestral clades, numerous new genes with simplified structures constrained expression patterns emerged, expressed predominantly coexpressed ancient drive glands. Among these, SpiCE-DS8 is newly evolved small secretory peptide, which incorporated into dragline involved MaSp network. SpiCE-DS8 originated de novo intergenic regions an orphan gene unique Nephilinae subfamily. It interacts N-terminal region MaSp1b, potentially aiding protein solidification. In vitro wet-spinning experiments demonstrated that significantly improved fiber properties, including β-sheet content mechanical strength, conserved SpiCE-DS8-S showing even more pronounced effects. enhanced properties allow arm their for efficient prey capture. This study highlights pivotal role lineage-specific gland evolution, providing insights mechanisms associated advancing synthetic material development.

Language: Английский

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A high-protein diet-responsive gut hormone regulates behavioral and metabolic optimization in Drosophila melanogaster

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Dec. 30, 2024

Protein is essential for all living organisms; however, excessive protein intake can have adverse effects, such as hyperammonemia. Although mechanisms responding to deficiency are well-studied, there a significant gap in our understanding of how organisms adaptively suppress intake. In the present study, utilizing fruit fly, Drosophila melanogaster, we discover that peptide hormone CCHamide1 (CCHa1), secreted by enteroendocrine cells response high-protein diet (HPD), vital suppressing overconsumption protein. Gut-derived CCHa1 received small subset enteric neurons produce short neuropeptide F, thereby modulating protein-specific satiety. Importantly, impairment CCHa1-mediated gut-enteric neuronal axis results ammonia accumulation and shortened lifespan under HPD conditions. Collectively, findings unravel crosstalk gut pathways orchestrate physiological responses prevent adapt dietary overload.

Language: Английский

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Exposure to a more unhealthy diet impacts sleep microstructure during normal sleep and recovery sleep: A randomized trial

Obesity, Journal Year: 2023, Volume and Issue: 31(7), P. 1755 - 1766

Published: May 28, 2023

Abstract Objective Although intake of specific macronutrients has been associated with sleep parameters, interventional evidence is lacking. Therefore, this randomized trial was conducted to examine how a more unhealthy high‐fat/high‐sugar (HFHS) diet impacts in humans. Methods In crossover study, 15 healthy young men consumed two isocaloric diets random order for week: an HFHS and low‐fat/low‐sugar diet. Following each diet, in‐lab recorded using polysomnography during full night recovery after extended wakefulness. Sleep duration, macrostructure, microstructure (oscillatory pattern slow waves) were investigated machine learning‐based algorithms. Results duration did not differ across the based on actigraphy polysomnography. macrostructure similar 1 week Compared consumption resulted reduced delta power, beta ratio, wave amplitude but increased alpha theta power deep sleep. During sleep, oscillatory changes observed. Conclusions Short‐term alters features that regulate restorative properties Whether such can mediate adverse health outcomes unhealthier warrants investigation.

Language: Английский

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