Dragonfly: A Portable Molecular Diagnostic Platform for Rapid Point-of-Care Detection of Mpox and Other Diseases DOI Creative Commons
Jesús Rodríguez-Manzano, Matthew L. Cavuto, Kenny Malpartida-Cardenas

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 20, 2024

Abstract The explosive global spread of Clade II mpox in humans and sustained human-to-human transmission the more virulent I Democratic Republic Congo highlight both current lack of, critical need for, point-of-care diagnostics to address this emergent disease. A novel molecular diagnostic platform, Dragonfly, was developed that integrates power-free nucleic acid extraction (< 5 minutes) lyophilized colorimetric LAMP chemistry. platform achieved an analytical limit-of-detection 50 genome copies/reaction for monkeypox virus, distinguishing it from other orthopoxviruses, herpes simplex virus varizella zoster virus. Clinical validation using 164 samples, including 51 positives, demonstrated 96.1% sensitivity 100% specificity 94.1% Our provides a rapid, accessible robust solution monitoring low high resource settings amidst declining worldwide smallpox immunity, with broad potential wider field infectious disease diagnosis as whole.

Language: Английский

Ultrasensitive single-step CRISPR detection of monkeypox virus in minutes with a vest-pocket diagnostic device DOI Creative Commons
Yunxiang Wang, Hong Chen, Kai Lin

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: April 16, 2024

Abstract The emerging monkeypox virus (MPXV) has raised global health concern, thereby highlighting the need for rapid, sensitive, and easy-to-use diagnostics. Here, we develop a single-step CRISPR-based diagnostic platform, termed SCOPE (Streamlined CRISPR On Pod Evaluation platform), field-deployable ultrasensitive detection of MPXV in resource-limited settings. viral nucleic acids are rapidly released from rash fluid swab, oral saliva, urine samples 2 min via streamlined lysis protocol, followed by 10-min recombinase polymerase amplification (RPA)-CRISPR/Cas13a reaction. A pod-shaped vest-pocket analysis device achieves whole process reaction execution, signal acquisition, result interpretation. can detect as low 0.5 copies/µL (2.5 copies/reaction) within 15 sample input to answer. We validate developed assay on 102 clinical male patients / volunteers, testing results 100% concordant with real-time PCR. single-molecular level sensitivity minutes simplified procedure performed miniaturized wireless device, which is expected spur substantial progress enable practice application diagnostics techniques point-of-care setting.

Language: Английский

Citations

34
Portable molecular diagnostic platform for rapid point-of-care detection of mpox and other diseases DOI Creative Commons
Matthew L. Cavuto, Kenny Malpartida-Cardenas, Ivana Pennisi

et al.

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: March 24, 2025

Abstract The World Health Organization’s designation of mpox as a public health emergency international concern in August 2024 underscores the urgent need for effective diagnostic solutions to combat this escalating threat. rapid global spread clade II mpox, coupled with sustained human-to-human transmission more virulent I Democratic Republic Congo, highlights critical gap point-of-care diagnostics emergent disease. In response, we developed Dragonfly, portable molecular platform use that integrates power-free nucleic acid extraction (<5 minutes) lyophilised colourimetric LAMP chemistry. demonstrated an analytical limit-of-detection 100 genome copies per reaction monkeypox virus, effectively distinguishing it from other orthopoxviruses, herpes simplex and varicella-zoster virus. Clinical validation on 164 samples, including 51 mpox-positive cases, yielded 96.1% sensitivity 100% specificity 94.1% Here, present rapid, accessible, robust solution suitable both low- high-resource settings, addressing resurgence orthopoxviruses context declining smallpox immunity.

Language: Английский

Citations

1
How fast are viruses spreading in the wild? DOI Creative Commons
Simon Dellicour, Paul Bastide,

Pauline Rocu

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: April 11, 2024

Abstract Genomic data collected from viral outbreaks can be exploited to reconstruct the dispersal history of lineages in a two-dimensional space using continuous phylogeographic inference. These spatially explicit reconstructions subsequently used estimate metrics allowing unveil dynamics and evaluate capacity spread among hosts. Heterogeneous sampling intensity genomic sequences however impact accuracy insights gained through In our study, we implement simulation framework robustness three — lineage velocity, diffusion coefficient, an isolation-by-distance signal metric effort. Our results reveal that both coefficient appear robust number samples considered for reconstruction. We then use these two compare pattern various viruses spreading animal populations. comparative analysis reveals broad range patterns coefficients mostly reflecting main infected host species but also, some cases, likely signature rapid and/or long-distance events driven by human-mediated movements trade. Overall, study provides key recommendations consider future studies illustrates their application settings.

Language: Английский

Citations

6
Impact of vaccination with third generation modified vaccinia Ankara and sexual behaviour on mpox incidence in men who have sex with men: analysis among participants of the ANRS-174 DOXYVAC trial DOI
Jade Ghosn, Lambert Assoumou,

Moussa Ouattara

et al.

The Lancet Regional Health - Europe, Journal Year: 2024, Volume and Issue: 45, P. 101020 - 101020

Published: Aug. 1, 2024

Language: Английский

Citations

6
Short-Lived Neutralizing Antibody Responses to Monkeypox Virus in Smallpox Vaccine–Naive Persons after JYNNEOS Vaccination DOI

Kara Phipps,

Jennifer L. Yates,

John E. Pettit

et al.

Emerging infectious diseases, Journal Year: 2025, Volume and Issue: 31(2)

Published: Jan. 10, 2025

JYNNEOS, a third-generation smallpox vaccine, is integral to monkeypox virus (MPXV) control efforts, but the durability of this modified vaccinia Ankara-Bavarian Nordic (MVA-BN) vaccine's effectiveness undefined. We optimized and used plaque reduction neutralization test (PRNT) with authentic clade IIa MPXV assess antibody responses over 12 months in 8 donors vaccinated 2 doses JYNNEOS. One donor previously received ACAM2000 vaccine; 7 were vaccine-naive. IgG (L1, B5, A33) or (E8, H3, A35) antigens PRNT titers both viruses peaked at weeks postvaccination waned rapidly thereafter naive donors. especially low; no demonstrated 90% reduction. These data indicate need for improved correlates immunity enable MVA-BN studies, given that recent clinical support vaccine efficacy against despite low responses.

Language: Английский

Citations

0
Development and validation of a quantitative Orthopoxvirus immunoassay to evaluate and differentiate serological responses to Mpox infection and vaccination DOI Creative Commons
Joanne Byrne, Gurvin Saini, A. Léon

et al.

EBioMedicine, Journal Year: 2025, Volume and Issue: 113, P. 105622 - 105622

Published: Feb. 22, 2025

Language: Английский

Citations

0
Attenuation of the 2022 global outbreak monkeypox virus relative to its clade IIb ancestor DOI Creative Commons
Rebecca P. Sumner, Lucy Eke, Bruno Hernáez

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 28, 2025

Abstract Monkeypox virus (MPXV) is a zoonotic endemic to Africa that has recently re-emerged, becoming the first orthopoxvirus with human-to-human transmission since smallpox eradication. The 2022 global epidemic of MPXV was caused by Clade IIb lineage B.1 derives from A.1, which West 1-3 . Here we compared an early its closest earlier A.1 ancestor and show despite only 46 nucleotide differences, attenuating phenotypic changes. Both viruses replicated equivalently in cell culture, but had defects long-range spread. also displayed innate immune control, inducing higher IFNβ, signalling MAPK gene expression, reduced capacity suppress interleukin-1β-mediated responses. Finally, virulence mouse model infection relative ancestral strain. Our work demonstrates biological differences between human circulating lineages correlate clinical observations documenting in-host viral dissemination disease severity for B. data reveal APOBEC3-like mutational footprint not signature sustained can drive

Language: Английский

Citations

0
Transmission dynamics of the 2022 mpox epidemic in New York City DOI Creative Commons
Jonathan E. Pekar, Yu Wang, Jade Wang

et al.

Nature Medicine, Journal Year: 2025, Volume and Issue: unknown

Published: March 25, 2025

Abstract The 2022 global mpox epidemic was caused by transmission of MPXV clade IIb, lineage B.1 through sexual contact networks, with New York City (NYC) experiencing the first and largest outbreak in United States. By performing phylogeographic analysis genomes sampled from 757 individuals NYC between April 2023, 3,287 around world, we identify over 200 introductions into at least 84 leading to onward transmission. These infections primarily occurred among men who have sex men, transgender women nonbinary individuals. Through a comparative HIV NYC, find that both genomic cluster sizes are best fit scale-free distributions, people clusters more likely previously received an diagnosis be member recently growing cluster. We model networks show highly connected would disproportionately infected start epidemic, which result exhaustion most densely parts network, and, therefore, explain rapid expansion decline outbreak. coupling epidemiology modeling, demonstrate dynamics can understood general principles sexually transmitted pathogens.

Language: Английский

Citations

0
Mobile Mpox Vaccination in New York City Provided Flexible Community-Responsive Vaccine Access During the 2022 Global Mpox Emergency DOI Creative Commons
Joseph Osmundson,

Julian L. Watkins,

Ashwin Vasan

et al.

Open Forum Infectious Diseases, Journal Year: 2025, Volume and Issue: 12(4)

Published: March 27, 2025

Abstract Background In May 2022, mpox (formerly monkeypox) began spreading globally through LGBTQ+ sexual networks. By August New York City (NYC) became the global epicenter of outbreak, with highest number cases reported in United States. Here, we quantify vaccination effort, focusing on flexible and community-responsive mobile vaccination. Methods We describe an on-site strategy at commercial sex venues, nightlife pride health centers, during 1–November 15, 2022. Data were collected doses, demographics, event size to determine evaluate vaccine uptake. Results The resulted 3358 JYNNEOS doses administered 363 events 58 locations, including 22 2 venues. Commercial venues closed height epidemic. show high uptake as many 60% attendees 1 receiving a dose site. This was possible after agencies responded community demand for second doses. Messaging about importance availability these parties community-led. via clinics demonstrated less racial geographic disparity compared nonmobile vaccinations. no increase reopened Conclusions These results demonstrate success community-led rapid response emergent places where people meet sex.

Language: Английский

Citations

0
Monkeypox Virus: WHO's Second Public Health Emergency of International Concern Within 2 Years DOI Creative Commons
Harald Brüssow

Microbial Biotechnology, Journal Year: 2025, Volume and Issue: 18(4)

Published: April 1, 2025

ABSTRACT An upsurge of monkeypox disease (mpox) cases with clade I virus in Central Africa led WHO to declare a Public Health Emergency International Concern for second time shortly after the worldwide II mpox epidemic 2022/3 among homosexual men. In Democratic Republic Congo (DRC), annual incidence mpox, transmitted mostly from animal sources children, increased 20‐fold between 1980 and 2007; 60,000 occurred 2010 2023. The again doubled 2023 2024, showing case fatality rate 3.3%. A new Ib was detected 2024 eastern DRC where adults were infected by heterosexual contact. recently introduced showed mutation spectrum human‐to‐human transmission. Asymptomatic infections, release infectious before symptom onset subgroup cases, superspreaders complicate containment measures during 2022 epidemic. Isolation until two consecutive negative PCR tests recommended but necessitates cheap rapid diagnostic which are development. Sexual behavioural changes have contributed more curbing than vaccination. smallpox vaccine Dryvax protected children exposed 1980s. attenuated third‐generation Modified Vaccinia Ankara (MVA) vaccines derivatives robust protection against IIb various study formats. Vaccine efficacy exceeding 75% reported doses. mRNA lipid‐nanoparticle encoding surface proteins extracellular enveloped intracellular mature virions (MPXV) induced humoral cellular immune responses that macaques viruses better MVA. Only mixtures monoclonal antibodies mice mpox. antiviral tecovirimat no clinical trials

Language: Английский

Citations

0