Current status of embryo models created from pluripotent stem cells DOI Creative Commons

Maria Carolina Zimara,

Toshihiko Ezashi, Ye Yuan

et al.

Reproduction Fertility and Development, Journal Year: 2024, Volume and Issue: 37(1)

Published: Dec. 12, 2024

The mystery of genesis has fascinated human inquiry ever since Aristotle in the 4th century BCE and likely even earlier. Observations carcinomas last led to derivation embryonic stem cells induced pluripotent cells. These remarkable recently allowed scientists develop embryo models that emulate natural developmental behavior embryos. In this review, we present how line research is unfolding, discuss its applications vitro fertilization, regenerative medicine, animal reproduction conservation. As navigate frontier, continues inspire challenge, shaping our understanding life’s fundamental processes opening doors unprecedented cell technologies.

Language: Английский

Pluripotent cell states and fates in human embryo models DOI
Berna Sözen, Patrick Tam, Martín F. Pera

et al.

Development, Journal Year: 2025, Volume and Issue: 152(7)

Published: April 1, 2025

ABSTRACT Pluripotency, the capacity to generate all cells of body, is a defining property transient population epiblast found in pre-, peri- and post-implantation mammalian embryos. As development progresses, undergo dynamic transitions pluripotency states, concurrent with specification extra-embryonic embryonic lineages. Recently, stem cell-based models pre- human have been developed using that capture key properties at different developmental stages. Here, we review early primate development, comparing states vivo cultured pluripotent representative these states. We consider how status starting influences embryo and, turn, what can learn about epiblast. Finally, discuss limitations questions arising from pioneering studies this emerging field.

Language: Английский

Citations

1
Advancing stem cell technologies for conservation of wildlife biodiversity DOI Creative Commons
Ashlee M. Hutchinson, Ruth Appeltant, Tom Burdon

et al.

Development, Journal Year: 2024, Volume and Issue: 151(20)

Published: Oct. 9, 2024

ABSTRACT Wildlife biodiversity is essential for healthy, resilient and sustainable ecosystems. For biologists, this diversity also represents a treasure trove of genetic, molecular developmental mechanisms that deepen our understanding the origins rules life. However, rapid decline in reported recently foreshadows potentially catastrophic collapse many important ecosystems associated irreversible loss forms life on planet. Immediate action by conservationists all stripes required to avert disaster. In Spotlight, we draw together insights proposals discussed at recent workshop hosted Revive & Restore, which gathered experts discuss how stem cell technologies can support traditional conservation techniques help protect animal biodiversity. We reprogramming, vitro gametogenesis, disease modelling embryo modelling, highlight prospects leveraging beyond mammalian species.

Language: Английский

Citations

7
Recent insights into the in vitro culture systems for mammalian embryos DOI
Zhiyuan Guo, Wentao Zhao, Hongmei Wang

et al.

Current Opinion in Genetics & Development, Journal Year: 2025, Volume and Issue: 91, P. 102309 - 102309

Published: Jan. 18, 2025

Language: Английский

Citations

0
Interspecies Blastocyst Complementation and the Genesis of Chimeric Solid Human Organs DOI Open Access
Enrico Bigliardi, Anala V. Shetty, Walter C. Low

et al.

Genes, Journal Year: 2025, Volume and Issue: 16(2), P. 215 - 215

Published: Feb. 12, 2025

Solid organ transplantation remains a life-saving treatment for patients worldwide. Unfortunately, the supply of donor organs cannot meet current need, making search alternative sources even more essential. Xenotransplantation using sophisticated genetic engineering techniques to delete and overexpress specific genes in animal has been investigated as possible option. However, use exogenous tissue presents another host obstacles, particularly regarding rejection. Given these limitations, interspecies blastocyst complementation combination with precise gene knockouts unique, promising pathway transplant shortage. In recent years, great advancements have made field, encouraging results producing donor-derived chimeric host. That said, one major barriers successful chimerism is mismatch developmental stages cells embryo. Another barrier speeds between embryos. This review outlines 19 studies which was used generate solid organs. particular, genesis liver, lung, kidney, pancreas, heart, thyroid, thymus parathyroids investigated. Of studies, 7 included an model. 7, completed human pig host, all others were rat-mouse chimeras. While very demonstrated, several challenges continue persist. chimerism, generation contribution, synchronized donor-host development, well ethical concerns human-animal chimeras remain important aspects that will need be addressed future research.

Language: Английский

Citations

0
Pluripotent stem cell–derived mesenchymal stem cells for therapeutic applications, developmental study, and cancer research DOI
Siyi Fu,

Cheung Kwan Yeung,

Ren‐He Xu

et al.

Current Opinion in Genetics & Development, Journal Year: 2025, Volume and Issue: 92, P. 102327 - 102327

Published: March 6, 2025

Language: Английский

Citations

0
Deep Learning-based Multi-Brain Capsule network for Next-Gen Clinical Emotion Recognition using EEG Signals DOI Creative Commons
Ravinder Dahiya,

G Mamatha,

Shila Jawale

et al.

Neuroscience Informatics, Journal Year: 2025, Volume and Issue: 5(2), P. 100203 - 100203

Published: April 28, 2025

Language: Английский

Citations

0
Incompatibility in cell adhesion constitutes a barrier to interspecies chimerism DOI
Emily Ballard, Masahiro Sakurai,

Leqian Yu

et al.

Cell stem cell, Journal Year: 2024, Volume and Issue: 31(10), P. 1419 - 1426.e7

Published: Aug. 23, 2024

Language: Английский

Citations

1
Current status of embryo models created from pluripotent stem cells DOI Creative Commons

Maria Carolina Zimara,

Toshihiko Ezashi, Ye Yuan

et al.

Reproduction Fertility and Development, Journal Year: 2024, Volume and Issue: 37(1)

Published: Dec. 12, 2024

The mystery of genesis has fascinated human inquiry ever since Aristotle in the 4th century BCE and likely even earlier. Observations carcinomas last led to derivation embryonic stem cells induced pluripotent cells. These remarkable recently allowed scientists develop embryo models that emulate natural developmental behavior embryos. In this review, we present how line research is unfolding, discuss its applications vitro fertilization, regenerative medicine, animal reproduction conservation. As navigate frontier, continues inspire challenge, shaping our understanding life’s fundamental processes opening doors unprecedented cell technologies.

Language: Английский

Citations

0