Communication from Learned to Innate Olfactory Processing Centers Is Required for Memory Retrieval in Drosophila DOI Creative Commons
Michael-John Dolan,

Ghislain Belliart-Guérin,

Alexander Shakeel Bates

et al.

Neuron, Journal Year: 2018, Volume and Issue: 100(3), P. 651 - 668.e8

Published: Sept. 20, 2018

The behavioral response to a sensory stimulus may depend on both learned and innate neuronal representations. How these circuits interact produce appropriate behavior is unknown. In Drosophila, the lateral horn (LH) mushroom body (MB) are thought mediate olfactory behavior, respectively, although LH function has not been tested directly. Here we identify two cell types (PD2a1 PD2b1) that receive input from an MB output neuron required for recall of aversive memories. These neurons memory retrieval modulated by training. Connectomics data demonstrate PD2a1 PD2b1 also direct food odor-encoding neurons. Consistent with this, necessary unlearned attraction some odors, indicating have dual role. This provides circuit mechanism which information can in identified behavior. VIDEO ABSTRACT.

Language: Английский

Chemoreceptor co-expression in Drosophila melanogaster olfactory neurons DOI Creative Commons
Darya Task, Chun‐Chieh Lin, Alina Vulpe

et al.

eLife, Journal Year: 2022, Volume and Issue: 11

Published: April 20, 2022

olfactory neurons have long been thought to express only one chemosensory receptor gene family. There are two main families in

Language: Английский

Citations

102
Drosophila glia: Few cell types and many conserved functions DOI Open Access
Kerem Yildirim,

Johanna Petri,

Rita Kottmeier

et al.

Glia, Journal Year: 2018, Volume and Issue: 67(1), P. 5 - 26

Published: Nov. 15, 2018

Abstract Glial cells constitute without any dispute an essential element in providing efficiently operating nervous system. Work many labs over the last decades has demonstrated that neuronal function, from action potential generation to its propagation, eliciting synaptic responses subsequent postsynaptic integration, is evolutionarily highly conserved. Likewise, biology of glial appears conserved core elements and therefore, a deeper understanding expected benefit analyzing model organisms such as Drosophila melanogaster . particularly well suited for studying since fly system only limited number exists, which can be individually identified based on position set molecular markers. In combination with well‐known genetic tool box unprecedented level analysis feasible, not help identify novel molecules principles governing cell function but also will better understand functions first mammalian Here we review current knowledge glia spark interest using this analyze complex traits future.

Language: Английский

Citations

160
Organization of olfactory centres in the malaria mosquito Anopheles gambiae DOI Creative Commons
Olena Riabinina, Darya Task, Elizabeth E. Marr

et al.

Nature Communications, Journal Year: 2016, Volume and Issue: 7(1)

Published: Oct. 3, 2016

Abstract Mosquitoes are vectors for multiple infectious human diseases and use a variety of sensory cues (olfactory, temperature, humidity visual) to locate host. A comprehensive understanding the circuitry underlying signalling in mosquito brain is lacking. Here we used Q-system binary gene expression develop transgenic lines Anopheles gambiae which olfactory receptor neurons expressing odorant co-receptor ( Orco ) labelled with GFP. These project from antennae maxillary palps antennal lobe (AL) labella on proboscis suboesophageal zone (SEZ), suggesting integration gustatory signals occurs this region. We present detailed anatomical maps innervations AL SEZ, identifying glomeruli that may respond body odours or carbon dioxide. Our results pave way functional neurogenetic studies processing mosquitoes.

Language: Английский

Citations

157
A genetic toolkit for tagging intronic MiMIC containing genes DOI Creative Commons
Sonal Nagarkar-Jaiswal, Steven DeLuca,

Pei-Tseng Lee

et al.

eLife, Journal Year: 2015, Volume and Issue: 4

Published: June 23, 2015

Previously, we described a large collection of Minos-Mediated Integration Cassettes (MiMICs) that contain two phiC31 recombinase target sites and allow the generation new exon encodes protein tag when MiMIC is inserted in codon intron (<xref ref-type="bibr" rid="bib17">Nagarkar-Jaiswal et al., 2015</xref>). These modified genes permit numerous applications including assessment expression pattern, identification interaction partners by immunoprecipitation followed mass spec, reversible removal tagged any tissue. At present, these conversions remain time labor-intensive as they require embryos to be injected with plasmid DNA containing tag. In this study, describe simple reliable genetic strategy genes/proteins insertions using an integrated encoding GFP flanked FRT sequences. We document efficiency 60 mostly uncharacterized genes.

Language: Английский

Citations

151
A complex peripheral code for salt taste in Drosophila DOI Creative Commons

Alexandria Jaeger,

Molly Stanley, Zachary F. Weiss

et al.

eLife, Journal Year: 2018, Volume and Issue: 7

Published: Oct. 11, 2018

Each taste modality is generally encoded by a single, molecularly defined, population of sensory cells. However, salt stimulates multiple pathways in mammals and insects, suggesting more complex code for taste. Here, we examine coding Drosophila. After creating comprehensive molecular map comprised five discrete neuron classes across the fly labellum, find that four are activated salt: two exhibiting characteristics ‘low salt’ cells, ‘high classes. Behaviorally, low attraction depends primarily on ‘sweet’ neurons, with additional input from neurons expressing ionotropic receptor IR94e. High avoidance mediated ‘bitter’ glutamatergic Ppk23. Interestingly, impact these prior consumption. These results support model flies combinatorially integrates inputs cell types to afford robust flexible behaviors.

Language: Английский

Citations

150
Excitation and inhibition onto central courtship neurons biases Drosophila mate choice DOI Creative Commons

Benjamin R. Kallman,

Hee‐Soo Kim, Kristin Scott

et al.

eLife, Journal Year: 2015, Volume and Issue: 4

Published: Nov. 14, 2015

The ability to distinguish males from females is essential for productive mate selection and species propagation. Recent studies in Drosophila have identified different classes of contact chemosensory neurons that detect female or male pheromones influence courtship decisions. Here, we examine central neural pathways the brain process using anatomical, calcium imaging, optogenetic, behavioral studies. We find sensory pheromones, but not activate a novel class ventral nerve cord cause activation P1 neurons, male-specific command trigger courtship. In addition, as well those mAL inhibit P1. These demonstrate balance excitatory inhibitory drives onto courtship-promoting controls mating

Language: Английский

Citations

146
Neurochemical Organization of the Drosophila Brain Visualized by Endogenously Tagged Neurotransmitter Receptors DOI Creative Commons
Shu Kondo, Takahiro Takahashi, Nobuhiro Yamagata

et al.

Cell Reports, Journal Year: 2020, Volume and Issue: 30(1), P. 284 - 297.e5

Published: Jan. 1, 2020

Neurotransmitters often have multiple receptors that induce distinct responses in receiving cells. Expression and localization of neurotransmitter individual neurons are therefore critical for understanding the operation neural circuits. Here we describe a comprehensive library reporter strains which convertible T2A-GAL4 cassette is inserted into endogenous receptor genes Drosophila. Using this library, profile expression 75 brain. Cluster analysis reveals neurochemical segmentation brain, distinguishing higher brain centers from rest. By recombinase-mediated exchange, convert split-GFP Tango to visualize subcellular activation dopamine specific cell types. This striking differences their localization, may underlie cellular different behavioral contexts. Our resources thus provide versatile toolkit dissecting organization function systems fly

Language: Английский

Citations

137
Representations of Novelty and Familiarity in a Mushroom Body Compartment DOI Creative Commons
Daisuke Hattori, Yoshinori Aso, Kurtis J. Swartz

et al.

Cell, Journal Year: 2017, Volume and Issue: 169(5), P. 956 - 969.e17

Published: May 1, 2017

Language: Английский

Citations

135
Sex Differences in Intestinal Carbohydrate Metabolism Promote Food Intake and Sperm Maturation DOI Creative Commons
Bruno Hudry,

Eva de Goeij,

Alessandro Mineo

et al.

Cell, Journal Year: 2019, Volume and Issue: 178(4), P. 901 - 918.e16

Published: Aug. 1, 2019

Physiology and metabolism are often sexually dimorphic, but the underlying mechanisms remain incompletely understood. Here, we use intestine of Drosophila melanogaster to investigate how gut-derived signals contribute sex differences in whole-body physiology. We find that carbohydrate handling is male-biased a specific portion intestine. In contrast known sexual dimorphisms invertebrates, intestinal extrinsically controlled by adjacent male gonad, which activates JAK-STAT signaling enterocytes within this portion. Sex reversal experiments establish roles for metabolic state controlling food intake sperm production through citrate. Our work uncovers gonad-gut axis coupling diet production, revealing communication across organs physiologically important. The instructive role citrate inter-organ might be significant more biological contexts than previously recognized.

Language: Английский

Citations

135
Neurotransmitter identity is acquired in a lineage-restricted manner in the Drosophila CNS DOI Creative Commons
Haluk Lacin, Hui-Min Chen, Xi Long

et al.

eLife, Journal Year: 2019, Volume and Issue: 8

Published: March 26, 2019

The vast majority of the adult fly ventral nerve cord is composed 34 hemilineages, which are clusters lineally related neurons. Neurons in these hemilineages use one three fast-acting neurotransmitters (acetylcholine, GABA, or glutamate) for communication. We generated a comprehensive neurotransmitter usage map entire cord. did not find any cases neurons using more than neurotransmitter, but found that acetylcholine specific gene ChAT transcribed many glutamatergic and GABAergic neurons, transcripts typically do leave nucleus translated. Importantly, our work uncovered simple rule: All within hemilineage same neurotransmitter. Thus, identity acquired at stem cell level. Our detailed transmitter- usage/lineage will be great resource studying developmental basis behavior deciphering how neuronal circuits function to regulate behavior.

Language: Английский

Citations

118