Cerebrospinal fluid can exit into the skull bone marrow and instruct cranial hematopoiesis in mice with bacterial meningitis

Nature Neuroscience, Journal Year: 2022, Volume and Issue: 25(5), P. 567 - 576

Published: May 1, 2022

Language: Английский

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A hybrid open-top light-sheet microscope for versatile multi-scale imaging of cleared tissues

Nature Methods, Journal Year: 2022, Volume and Issue: 19(5), P. 613 - 619

Published: May 1, 2022

Language: Английский

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Engineering brain assembloids to interrogate human neural circuits

Nature Protocols, Journal Year: 2022, Volume and Issue: 17(1), P. 15 - 35

Published: Jan. 1, 2022

Language: Английский

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Tissue clearing

Nature Reviews Methods Primers, Journal Year: 2021, Volume and Issue: 1(1)

Published: Dec. 16, 2021

Language: Английский

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Whole-mouse clearing and imaging at the cellular level with vDISCO

Nature Protocols, Journal Year: 2023, Volume and Issue: 18(4), P. 1197 - 1242

Published: Jan. 25, 2023

Language: Английский

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Achieving optical transparency in live animals with absorbing molecules

Science, Journal Year: 2024, Volume and Issue: 385(6713)

Published: Sept. 5, 2024

Optical imaging plays a central role in biology and medicine but is hindered by light scattering live tissue. We report the counterintuitive observation that strongly absorbing molecules can achieve optical transparency animals. explored physics behind this found when dissolve water, they modify refractive index of aqueous medium through Kramers-Kronig relations to match high-index tissue components such as lipids. have demonstrated our straightforward approach reversibly render mouse body transparent allow visualization wide range deep-seated structures activities. This work suggests search for high-performance clearing agents should focus on molecules.

Language: Английский

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Patterning and folding of intestinal villi by active mesenchymal dewetting

Cell, Journal Year: 2024, Volume and Issue: 187(12), P. 3072 - 3089.e20

Published: May 22, 2024

Tissue folds are structural motifs critical to organ function. In the intestine, bending of a flat epithelium into periodic pattern gives rise villi, finger-like protrusions that enable nutrient absorption. However, molecular and mechanical processes driving villus morphogenesis remain unclear. Here, we identify an active mechanism simultaneously patterns intestinal initiate formation. At cellular level, find PDGFRA+ subepithelial mesenchymal cells generate myosin II-dependent forces sufficient produce patterned curvature in neighboring tissue interfaces. This symmetry-breaking process requires altered cell extracellular matrix interactions enabled by metalloproteinase-mediated fluidization. Computational models, together with vitro vivo experiments, revealed these features manifest at level as differences interfacial tensions promote aggregation interface through analogous dewetting thin liquid film.

Language: Английский

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Control of neuronal excitation–inhibition balance by BMP–SMAD1 signalling

Nature, Journal Year: 2024, Volume and Issue: 629(8011), P. 402 - 409

Published: April 17, 2024

Abstract Throughout life, neuronal networks in the mammalian neocortex maintain a balance of excitation and inhibition, which is essential for computation 1,2 . Deviations from balanced state have been linked to neurodevelopmental disorders, severe disruptions result epilepsy 3–5 To balance, microcircuits composed excitatory inhibitory neurons sense alterations neural activity adjust connectivity function. Here we identify signalling pathway adult mouse that activated response increased network activity. Overactivation signalled through an increase levels BMP2, growth factor well known its role as morphogen embryonic development. BMP2 acts on parvalbumin-expressing (PV) interneurons transcription SMAD1, controls array glutamatergic synapse proteins components perineuronal nets. PV-interneuron-specific disruption BMP2–SMAD1 accompanied by loss innervation PV cells, underdeveloped nets decreased excitability. Ultimately, this impairment functional recruitment disrupts cortical excitation–inhibition with mice exhibiting spontaneous epileptic seizures. Our findings suggest developmental repurposed stabilize brain.

Language: Английский

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Tissue clearing technique: Recent progress and biomedical applications

Journal of Anatomy, Journal Year: 2020, Volume and Issue: 238(2), P. 489 - 507

Published: Sept. 16, 2020

Organisms are inherently three dimensional, thus comprehensive understanding of the complicated biological system requires analysis organs or even whole bodies in context dimensions. However, this is a tremendous task since specimens naturally opaque, major obstacle whole-body and whole-organ imaging. Tissue clearing technique provides prospective solution has become powerful tool for three-dimensional imaging quantification organisms. aims to make tissue transparent by minimizing light scattering absorption, allowing deep large volume samples. When combined with diverse molecular labeling methods high-throughput optical sectioning microscopes, enables at cellular subcellular resolution, providing detailed information about intact systems. Here, we give an overview recent progress biomedical applications technique. We introduce mechanisms basic principles clearing, summarize current methods. Moreover, available techniques software packages data processing also presented. Finally, advances fields. contributes investigation structure-function relationships mammalian organs, opens new avenues mapping human organs. hope review better can help researchers select best-suited protocol their experiments.

Language: Английский

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Biomedical Applications of Tissue Clearing and Three-Dimensional Imaging in Health and Disease

iScience, Journal Year: 2020, Volume and Issue: 23(8), P. 101432 - 101432

Published: Aug. 1, 2020

Three-dimensional (3D) optical imaging techniques can expand our knowledge about physiological and pathological processes that cannot be fully understood with 2D approaches. Standard diagnostic tests frequently are not sufficient to unequivocally determine the presence of a condition. Whole-organ requires tissue transparency, which achieved by using clearing procedures enabling deeper image acquisition therefore making possible analysis large-scale biological samples. Here, we review currently available agents, methods, their application in or conditions different animal human organs. We also compare methods discussing advantages disadvantages use 3D for visualization cleared tissues. The resources tissues paves way future applications translational clinical research.

Language: Английский

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