Pharmaceutics,
Journal Year:
2023,
Volume and Issue:
15(3), P. 946 - 946
Published: March 15, 2023
Multiplexed
fluorescent
immunohistochemical
analysis
of
breast
cancer
(BC)
markers
and
high-resolution
3D
immunofluorescence
imaging
the
tumor
its
microenvironment
not
only
facilitate
making
disease
prognosis
selecting
effective
anticancer
therapy
(including
photodynamic
therapy),
but
also
provides
information
on
signaling
metabolic
mechanisms
carcinogenesis
helps
in
search
for
new
therapeutic
targets
drugs.
The
characteristics
nanoprobe
efficiency,
such
as
sensitivity,
target
affinity,
depth
tissue
penetration,
photostability,
are
determined
by
properties
their
components,
fluorophores
capture
molecules,
method
conjugation.
Regarding
individual
nanocrystals
(NCs)
widely
used
optical
vitro
vivo,
single-domain
antibodies
(sdAbs)
well
established
highly
specific
molecules
diagnostic
applications.
Moreover,
technologies
obtaining
functionally
active
sdAb–NC
conjugates
with
highest
possible
avidity,
all
sdAb
bound
to
NC
a
strictly
oriented
manner,
provide
3D-imaging
nanoprobes
strong
comparative
advantages.
This
review
is
aimed
at
highlighting
importance
an
integrated
approach
BC
diagnosis,
including
detection
biomarkers
microenvironment,
need
quantitative
profiling
mutual
location,
using
advanced
approaches
thick
sections.
existing
tumors
NCs
described,
main
advantages
disadvantages
nontoxic
multiplexed
discussed.
Frontiers in Neuroscience,
Journal Year:
2022,
Volume and Issue:
16
Published: March 25, 2022
The
brain
is
the
central
and
most
complex
organ
in
nervous
system,
comprising
billions
of
neurons
that
constantly
communicate
through
trillions
connections
called
synapses.
Despite
being
formed
mainly
during
prenatal
early
postnatal
development,
synapses
are
continually
refined
eliminated
throughout
life
via
complicated
hitherto
incompletely
understood
mechanisms.
Failure
to
correctly
regulate
numbers
distribution
has
been
associated
with
many
neurological
psychiatric
disorders,
including
autism,
epilepsy,
Alzheimer’s
disease,
schizophrenia.
Therefore,
measurements
synaptic
density,
as
well
detection
dysfunction,
essential
for
understanding
normal
abnormal
development.
To
date,
multiple
density
markers
have
proposed
investigated
experimental
models
disorders.
majority
gold
standard
methodologies
(e.g.,
electron
microscopy
or
immunohistochemistry)
visualize
measure
changes
pre-
postsynaptic
proteins
ex
vivo
.
However,
invasive
nature
these
classic
precludes
their
use
living
organisms.
recent
development
positron
emission
tomography
(PET)
tracers
[such
(
18
F)UCB-H
11
C)UCB-J]
bind
a
putative
marker,
vesicle
2A
(SV2A)
protein,
heralding
likely
paradigm
shift
detecting
alterations
patients.
limited
specificity,
novel,
non-invasive
magnetic
resonance
(MR)-based
methods
also
show
promise
inferring
information
by
linking
glutamate
neurotransmission.
Although
promising,
all
entail
various
advantages
limitations
must
be
addressed
before
becoming
part
routine
clinical
practice.
In
this
review,
we
summarize
discuss
current
quantifying
an
evaluation
reliability
utility.
We
conclude
critical
assessment
challenges
need
overcome
successfully
employing
biomarkers
diagnostic
and/or
prognostic
tools
study
neuropsychiatric
Development,
Journal Year:
2021,
Volume and Issue:
148(18)
Published: Sept. 15, 2021
ABSTRACT
Tissue
clearing
increases
the
transparency
of
late
developmental
stages
and
enables
deep
imaging
in
fixed
organisms.
Successful
implementation
these
methodologies
requires
a
good
grasp
sample
processing,
possibilities
offered
by
image
analysis.
In
this
Primer,
we
highlight
how
tissue
can
revolutionize
histological
analysis
processes
advise
on
to
implement
effective
protocols,
strategies
methods
for
biology.
PLoS Biology,
Journal Year:
2023,
Volume and Issue:
21(6), P. e3002167 - e3002167
Published: June 27, 2023
Technological
advancements
in
biology
and
microscopy
have
empowered
a
transition
from
bioimaging
as
an
observational
method
to
quantitative
one.
However,
biologists
are
adopting
these
experiments
become
more
complex,
researchers
need
additional
expertise
carry
out
this
work
rigorous
reproducible
manner.
This
Essay
provides
navigational
guide
for
experimental
aid
understanding
of
sample
preparation
through
image
acquisition,
analysis,
data
interpretation.
We
discuss
the
interconnectedness
steps,
each,
we
provide
general
recommendations,
key
questions
consider,
links
high-quality
open-access
resources
further
learning.
synthesis
information
will
empower
plan
execute
efficiently.
iScience,
Journal Year:
2021,
Volume and Issue:
24(4), P. 102387 - 102387
Published: April 1, 2021
Tissue
imaging
in
3D
using
visible
light
is
limited
and
various
clearing
techniques
were
developed
to
increase
depth,
but
none
provides
universal
solution
for
all
tissues
at
developmental
stages.
In
this
review,
we
focus
on
different
tissue
methods
of
heart
vasculature,
based
chemical
composition
(solvent-based,
simple
immersion,
hyperhydration,
hydrogel
embedding
techniques).
We
discuss
detail
compatibility
with
visualization
methods:
fluorescence
preservation,
immunohistochemistry,
nuclear
staining,
fluorescent
dyes
vascular
perfusion.
also
myocardium
autofluorescence,
shrinking,
expansion.
Then
overview
used
study
cardiovascular
system
live
imaging.
vessels
segmentation
image
analysis.
The
review
covers
the
whole
process
imaging,
starting
from
its
types
resulting
Immunological Reviews,
Journal Year:
2021,
Volume and Issue:
306(1), P. 137 - 163
Published: Dec. 3, 2021
Abstract
Dendritic
cells
(DCs)
are
crucial
for
the
appropriate
initiation
of
adaptive
immune
responses.
During
inflammation,
DCs
capture
antigens,
mature,
and
migrate
to
lymphoid
tissues
present
foreign
material
naïve
T
cells.
These
get
activated
differentiate
either
into
pathogen‐specific
cytotoxic
CD8
+
that
destroy
infected
or
CD4
helper
that,
among
other
effector
functions,
orchestrate
antibody
production
by
B
DC‐mediated
antigen
presentation
is
equally
important
in
non‐inflammatory
conditions.
Here,
mediate
induction
tolerance
presenting
self‐antigens
harmless
environmental
antigens
induce
differentiation
regulatory
inactivation
self‐reactive
Detailed
insights
biology
are,
therefore,
development
novel
vaccines
as
well
prevention
autoimmune
diseases.
As
many
life
science
areas,
our
understanding
DC
would
be
extremely
restricted
without
bioimaging,
a
compilation
methods
visualize
biological
processes.
Spatiotemporal
tracking
relies
on
various
imaging
tools,
which
not
only
enable
their
positioning
migration
within
entire
organs
but
also
allow
visualization
subcellular
molecular
This
review
aims
provide
an
overview
toolbox
examples
diverse
techniques
used
obtain
fundamental
biology.
