Single-Nucleus RNA Sequencing of Developing and Mature Superior Colliculus Identifies Neuronal Diversity and Candidate Mediators of Circuit Assembly

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Feb. 2, 2023

The superior colliculus (SC) is a sensorimotor structure in the midbrain that integrates input from multiple sensory modalities to initiate motor commands. It undergoes well-characterized steps of circuit assembly during development, rendering mouse SC popular model study establishment and refinement neural connectivity. Here we performed single nucleus RNA-sequencing analysis isolated at various developmental time points. Our provides transcriptomic landscape cell types comprise across murine development with particular emphasis on neuronal heterogeneity. We used these data identify Pax7 as marker for an anatomically homogeneous population GABAergic neurons. Lastly, report repertoire genes differentially expressed different postnatal ages, many which are known regulate axon guidance synapse formation. provide valuable resource interrogating mechanisms identifying markers manipulating specific populations circuits.

Language: Английский

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Mapping the Retina onto the Brain

Cold Spring Harbor Perspectives in Biology, Journal Year: 2023, Volume and Issue: 16(2), P. a041512 - a041512

Published: Dec. 5, 2023

Daniel Kerschensteiner1,2,3 and Marla B. Feller4,5 1Department of Ophthalmology Visual Sciences 2Department Neuroscience 3Department Biomedical Engineering, Washington University School Medicine, St. Louis, Missouri 63110, USA 4Department Molecular Cell Biology 5Helen Wills Institute, California, Berkeley, California 94720, Correspondence: mfeller{at}berkeley.edu; kerschensteinerd{at}wustl.edu

Language: Английский

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Enhancement and contextual modulation of visuospatial processing by thalamocollicular projections from ventral lateral geniculate nucleus

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: Nov. 10, 2023

Abstract In the mammalian visual system, ventral lateral geniculate nucleus (vLGN) of thalamus receives salient input from retina and sends prominent GABAergic axons to superior colliculus (SC). However, whether how vLGN contributes fundamental information processing remains largely unclear. Here, we report in mice that facilitates visually-guided approaching behavior mediated by SC enhances sensitivity object detection. This can be attributed extremely broad spatial integration neurons, as reflected their much lower preferred frequencies broader receptive fields than neurons. Through thalamocollicular projections, specifically exerts surround suppression visuospatial SC, leading a fine tuning preferences higher smaller objects context-dependent manner. Thus, an essential component central pathway, serves refine contextually modulate SC-mediated visuomotor behaviors via visually-driven long-range feedforward inhibition.

Language: Английский

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Pattern of Driver-Like Input onto Neurons of the Mouse Ventral Lateral Geniculate Nucleus

eNeuro, Journal Year: 2023, Volume and Issue: 10(1), P. ENEURO.0386 - 22.2022

Published: Jan. 1, 2023

Abstract The ventral lateral geniculate nucleus (vLGN) is a retinorecipient region of thalamus that contributes to number complex visual behaviors. Retinal axons target vLGN terminate exclusively in the external subdivision (vLGNe), which also transcriptionally and cytoarchitectonically distinct from internal (vLGNi). While recent studies shed light on cell types efferent projections vLGNe vLGNi, we have crude understanding source nature excitatory inputs driving postsynaptic activity these regions. Here, address this by conducting vitro whole-cell recordings acutely prepared thalamic slices using electrical optical stimulation techniques examine evoked activation retinal or cortical layer V input onto neurons vLGNi. Activation afferents optic tract terminals resulted robust driver-like vLGNe. Optical corticothalamic similar both Using dual-color optogenetic approach, found many received convergent two sources. Both individual pathways displayed properties, with leading stronger form synaptic depression than retinogeniculate stimulation. We no evidence convergence only responding These data provide insight into influence reveal receive

Language: Английский

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Glutamatergic and GABAergic neurons in the vLGN mediate the nociceptive effects of green and red light on neuropathic pain

Neurobiology of Disease, Journal Year: 2023, Volume and Issue: 183, P. 106164 - 106164

Published: May 20, 2023

Phototherapy is an emerging non-pharmacological treatment for depression, circadian rhythm disruptions, and neurodegeneration, as well pain conditions including migraine fibromyalgia. However, the mechanism of phototherapy-induced antinociception not understood. Here, using fiber photometry recordings population-level neural activity combined with chemogenetics, we found that phototherapy elicits via regulation ventral lateral geniculate body (vLGN) located in visual system. Specifically, both green red lights caused increase c-fos vLGN, light increased more. In causes a large glutamatergic neurons, whereas GABAergic neurons. Green preconditioning increases sensitivity neurons to noxious stimuli vLGN PSL mice. produces by activating promotes nociception vLGN. Together, these results demonstrate different colors exert modulation effects regulating subpopulations This may provide potential new therapeutic strategies targets precise clinical neuropathic pain.

Language: Английский

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