Reviewer #3 (Public Review): Heterogeneous presynaptic receptive fields contribute to directional tuning in starburst amacrine cells DOI Open Access

John A. Gaynes,

Samuel A. Budoff,

Michael J. Grybko

et al.

Published: Sept. 12, 2023

The processing of visual information by retinal starburst amacrine cells (SACs) involves transforming excitatory input from bipolar (BCs) into directional calcium output. While previous studies have suggested that an asymmetry in the kinetic properties along soma-dendritic axes postsynaptic cell could enhance tuning at level individual branches, it remains unclear whether biologically relevant presynaptic kinetics contribute to direction selectivity when stimulation engages entire dendritic tree. To address this question, we built multicompartmental models bipolar–SAC circuit and trained them boost tuning. We report despite significant crosstalk dissimilar preferences dendrites occur during whole-cell stimulation, rules guide BC leading optimal are similar single-dendrite condition. correlate model predictions empirical findings, utilized two-photon glutamate imaging study dynamics release onto ON- OFF-starburst murine retina. reveal diverse response motion both populations; algorithms on experimental data differences temporal likely correspond heterogeneous receptive field (RF) among different types, including spatial extent center surround components. In addition, demonstrate architecture composed units with experimentally recorded drive but not levels replicate suggesting other DS mechanisms required explain SAC function. Our provides new insights complex underlying highlights potential contribution computation cells.

Language: Английский

A presynaptic source drives differing levels of surround suppression in two mouse retinal ganglion cell types DOI Creative Commons
David Swygart, Wan‐Qing Yu, Shunsuke Takeuchi

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Jan. 18, 2024

Abstract In early sensory systems, cell-type diversity generally increases from the periphery into brain, resulting in a greater heterogeneity of responses to same stimuli. Surround suppression is canonical visual computation that begins within retina and found at varying levels across retinal ganglion cell types. Our results show level surround occurs subcellularly bipolar synapses. Using single-cell electrophysiology serial block-face scanning electron microscopy, we two types exhibit very different even though they receive input This divergence signal through synapse-specific regulation by amacrine cells scale tens microns. These findings indicate each synapse single can carry unique signal, expanding number possible functional channels earliest stages processing.

Language: Английский

Citations

8
The Interplay between Neurotransmitters and Calcium Dynamics in Retinal Synapses during Development, Health, and Disease DOI Open Access
Johane M. Boff, Abhishek P Shrestha,

Saivikram Madireddy

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(4), P. 2226 - 2226

Published: Feb. 13, 2024

The intricate functionality of the vertebrate retina relies on interplay between neurotransmitter activity and calcium (Ca

Language: Английский

Citations

5
Asymmetries in the Architecture of ON and OFF Arbors in ON–OFF Direction‐Selective Ganglion Cells DOI
Shaji Philip, Narendra Pratap Singh, Savin Viswanathan

et al.

The Journal of Comparative Neurology, Journal Year: 2025, Volume and Issue: 533(1)

Published: Jan. 1, 2025

ABSTRACT Direction selectivity is a fundamental feature in the visual system. In retina, direction independently computed by ON and OFF circuits. However, advantages of extracting directional information from these two independent circuits are unclear. To gain insights, we examined ON–OFF direction‐selective ganglion cells (DSGCs), which recombine signals both Specifically, investigated dendritic architecture neurons with premise that asymmetries will provide insights into function. Scrutinizing dendrites dye‐filled DSGCs reveals arbors substantially denser. The increase density can be primarily attributed to higher branching seen arbors. Further, analysis previously published serial block‐face electron microscopy dataset revealed denser packed more bipolar synapses per unit length. These suggest DSGC preferentially magnifies synaptic drive pathway, potentially allowing it encode distinct pathway.

Language: Английский

Citations

0
Two mechanisms for direction selectivity in a model of the primate starburst amacrine cell DOI Creative Commons
Wu Jiajia, Yeon Jin Kim, Dennis M. Dacey

et al.

Visual Neuroscience, Journal Year: 2023, Volume and Issue: 40

Published: Jan. 1, 2023

Abstract In a recent study, visual signals were recorded for the first time in starburst amacrine cells of macaque retina, and, as mouse and rabbit, directional bias observed calcium was from near dendritic tips. Stimulus motion soma toward tip generated larger signal than soma. Two mechanisms affecting spatiotemporal summation excitatory postsynaptic currents have been proposed to contribute signaling at tips starbursts: (1) “morphological” mechanism which electrotonic propagation synaptic along dendrite sums bipolar cell inputs preferentially stimulus centrifugal direction; (2) “space–time” that relies on differences time-courses proximal distal favor motion. To explore contributions these two primate, we developed realistic computational model based connectomic reconstruction distribution its sustained transient types. Our suggests both can initiate direction selectivity dendrites, but their differ depending properties stimulus. Specifically, morphological dominates when small objects are moving high velocities, space–time contributes most large low velocities.

Language: Английский

Citations

9
Neural extracellular matrix regulates visual sensory motor integration DOI Creative Commons
Jacqueline Reinhard,

Cornelius Mueller-Buehl,

Susanne Wiemann

et al.

iScience, Journal Year: 2024, Volume and Issue: 27(2), P. 108846 - 108846

Published: Jan. 9, 2024

Visual processing depends on sensitive and balanced synaptic neurotransmission. Extracellular matrix proteins in the environment of cells are key modulators synaptogenesis plasticity. In present study, we provide evidence that combined loss four extracellular components, brevican, neurocan, tenascin-C, tenascin-R, quadruple knockout mice leads to severe retinal dysfunction diminished visual motion

Language: Английский

Citations

3
A circuit motif for color in the human foveal retina DOI Creative Commons
Yeon Jin Kim, Orin Packer, Dennis M. Dacey

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2024, Volume and Issue: 121(36)

Published: Aug. 27, 2024

The neural pathways that start human color vision begin in the complex synaptic network of foveal retina where signals originating long (L), middle (M), and short (S) wavelength-sensitive cone photoreceptor types are compared through antagonistic interactions, referred to as opponency. In nonhuman primates, two opponent well established: an L vs. M circuit linked midget ganglion cell type, often called red-green pathway, S + small bistratified blue-yellow pathway. These have been taken correspond cardinal directions a trichromatic space, providing parallel inputs higher-level processing. Yet linking opponency primate mechanisms has proven particularly difficult. Here, we apply connectomic reconstruction trace excitatory outputs from S-ON (or “blue-cone”) bipolar additional types: large subpopulation ON-midget cells, whose connections suggest significant unique role vision. postsynaptic both cells thus define merge circuits, with potential for at first stage

Language: Английский

Citations

3
Distinct inhibitory pathways control velocity and directional tuning in the mouse retina DOI Creative Commons

Mathew T. Summers,

Marla B. Feller

Current Biology, Journal Year: 2022, Volume and Issue: 32(10), P. 2130 - 2143.e3

Published: April 7, 2022

The sensory periphery is responsible for detecting ethologically relevant features of the external world, using compact, predominantly feedforward circuits. Visual motion a particularly prevalent feature, presence which can be signal to enact diverse behaviors ranging from gaze stabilization reflexes predator avoidance or prey capture. To understand how retina constructs distinct neural representations required these behaviors, we investigated two circuits encoding different aspects image motion: ON and ON-OFF direction-selective ganglion cells (DSGCs). Using combination two-photon targeted whole-cell electrophysiology, pharmacology, conditional knockout mice, show that inhibitory pathways independently control tuning velocity direction in cell types. We further employ dynamic clamp numerical modeling techniques asymmetric inhibition provides velocity-invariant mechanism directional tuning, despite strong dependence classical models selectivity. therefore demonstrate invariant by interneurons act as computational building blocks construct distinct, behaviorally signals at earliest stages visual system.

Language: Английский

Citations

13
Neural Circuits Underlying Multifeature Extraction in the Retina DOI

Prathyusha Ravi Chander,

Laura Hanson, Pavitra Chundekkad

et al.

Journal of Neuroscience, Journal Year: 2023, Volume and Issue: 44(10), P. e0910232023 - e0910232023

Published: Nov. 13, 2023

Classic ON-OFF direction-selective ganglion cells (DSGCs) that encode the four cardinal directions were recently shown to also be orientation-selective. To clarify mechanisms underlying orientation selectivity, we employed a variety of electrophysiological, optogenetic, and gene knock-out strategies test relative contributions glutamate, GABA, acetylcholine (ACh) input are known drive DSGCs, in male female mouse retinas. Extracellular spike recordings revealed DSGCs respond preferentially either vertical or horizontal bars, those perpendicular their preferred-null motion axes. By contrast, glutamate all DSGC types measured using whole-cell patch-clamp techniques was found tuned along axis. Tuned glutamatergic excitation heavily reliant on type 5A bipolar cells, which appear electrically coupled via connexin 36 containing gap junctions vertically oriented processes wide-field amacrine cells. Vertically inputs transformed by GABAergic/cholinergic "starburst" (SACs), critical components circuit, into distinct patterns inhibition excitation. Feed-forward SAC appears "veto" preferred dorsal/ventral (but not nasal/temporal) coding "flipping" tuning 90° accounts for apparent mismatch between DSGC's spiking response. Together, these results reveal how two synaptic motifs interact generate complex feature shedding light intricate circuitry underlies visual processing retina.

Language: Английский

Citations

6
Tiny drone object detection in videos guided by the bio-inspired magnocellular computation model DOI
Gang Wang, Xin Yang, Liang Li

et al.

Applied Soft Computing, Journal Year: 2024, Volume and Issue: 163, P. 111892 - 111892

Published: Sept. 1, 2024

Language: Английский

Citations

2
Roles of visually evoked and spontaneous activity in the development of retinal direction selectivity maps DOI
Alexandre Tiriac, Marla B. Feller

Trends in Neurosciences, Journal Year: 2022, Volume and Issue: 45(7), P. 529 - 538

Published: April 29, 2022

Language: Английский

Citations

9