Single neurons and networks in the claustrum integrate input from widespread cortical sources DOI Open Access
Andrew M. Shelton, David K. Oliver,

Ivan P Lazarte

et al.

Published: Nov. 5, 2024

The claustrum is thought to be one of the most highly interconnected forebrain structures but its organizing principles have yet fully explored at level single neurons. Here, we investigated identity, connectivity, and activity identified neurons understand how structure’s unique convergence input divergence output support binding information streams. We found that in communicate with each other across efferent projection-defined modules which were differentially innervated by sensory frontal cortical areas. Individual responsive inputs from more than region a cell-type projection-specific manner, particularly between areas cortex. In vivo imaging axons revealed responses both unimodal multimodal stimuli. Finally, chronic silencing specifically reduced animals’ sensitivity These findings view fundamentally integrative structure, consolidating around cortex redistributing it following local computations.

Language: Английский

A multifaceted architectural framework of the mouse claustrum complex DOI Creative Commons
Joachim S. Grimstvedt, Andrew M. Shelton, Anna Hoerder‐Suabedissen

et al.

The Journal of Comparative Neurology, Journal Year: 2023, Volume and Issue: 531(17), P. 1772 - 1795

Published: Oct. 2, 2023

Accurate anatomical characterizations are necessary to investigate neural circuitry on a fine scale, but for the rodent claustrum complex (CLCX), this has yet be fully accomplished. The CLCX is generally considered comprise two major subdivisions, (CL) and dorsal endopiriform nucleus (DEn), regional boundaries these areas debated. To address this, we conducted multifaceted analysis of fiber- cytoarchitecture, genetic marker expression, connectivity using mice both sexes, create comprehensive guide identifying delineating borders CLCX, including an online reference atlas. Our data indicated four distinct subregions within subdividing CL DEn into two. Additionally, brain-wide tracing inputs transgenic mouse line. Immunohistochemical staining against myelin basic protein (MBP), parvalbumin (PV), calbindin (CB) revealed intricate fiber-architectural patterns enabling precise delineations its subregions. Myelinated fibers were abundant dorsally in absent ventrally, whereas PV expressing occupied entire CL. CB central gap CL, also visible anterior striatum. Nr2f2, Npsr1, Cplx3 genes expressed specifically different Rprm helped delineate CL-insular border. Furthermore, cells projecting retrosplenial cortex located sparse area. By combining own experimental with digitally available datasets gene expression input connectivity, could demonstrate that proposed delineation scheme allows anchoring from origins common framework.

Language: Английский

Citations

30
A simple and reliable method for claustrum localization across age in mice DOI Creative Commons
Tarek Shaker,

Gwyneth J. Dagpa,

Vanessa Cattaud

et al.

Molecular Brain, Journal Year: 2024, Volume and Issue: 17(1)

Published: Feb. 17, 2024

Abstract The anatomical organization of the rodent claustrum remains obscure due to lack clear borders that distinguish it from neighboring forebrain structures. Defining what constitutes is imperative for elucidating its functions. Methods based on gene/protein expression or transgenic mice have been used spatially outline but often report incomplete labeling and/or specificity during certain neurodevelopmental timepoints. To reliably identify projection cells in mice, we propose a simple immunolabelling method juxtaposes pattern claustrum-enriched and cortical-enriched markers. We determined immunoreactive markers Nurr1 Nr2f2 are devoid cortical marker Tle4, which allowed us differentiate adjoining cells. Using retrograde tracing, verified nearly all neurons Tle4 expressed Nurr1/Nr2f2 different degrees. At neonatal stages between 7 21 days, were identified by their Nurr1-postive/Tle4-negative profile, time-period when other techniques localize adult ineffective. Finally, exposure environmental novelty enhanced neuronal activation c-Fos region. Notably, was mainly restricted Nurr1-positive absent Tle4-positive cells, thus corroborating previous work reporting novelty-induced activation. Taken together, this will aid studying postnatal development may improve histological functional studies where approaches not amenable.

Language: Английский

Citations

7
Claustrum neurons projecting to the anterior cingulate restrict engagement during sleep and behavior DOI Creative Commons
Gal Atlan, Noa Matosevich,

Noa Peretz‐Rivlin

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: June 26, 2024

Abstract The claustrum has been linked to attention and sleep. We hypothesized that this reflects a shared function, determining responsiveness stimuli, which spans the axis of engagement. To test hypothesis, we recorded population dynamics from male mice during both sleep an attentional task (‘ENGAGE’). Heightened activity in neurons projecting anterior cingulate cortex (ACCp) corresponded reduced sensory Similarly, ENGAGE task, heightened ACCp correlated with disengagement behavioral lapses, while low hyper-engagement impulsive errors. Chemogenetic elevation awakenings errors task. Furthermore, employing exploration strategy showed stronger correlation between performance compared exploitation complexity. Our results implicate restricting engagement goal-directed behavior.

Language: Английский

Citations

7
Brain-state-dependent constraints on claustrocortical communication and function DOI Creative Commons
Brian A. Marriott,

D. Alison,

Coline Portet

et al.

Cell Reports, Journal Year: 2023, Volume and Issue: 43(1), P. 113620 - 113620

Published: Dec. 29, 2023

Neural activity in the claustrum has been associated with a range of vigilance states, yet patterns and efficacy synaptic communication identified neurons have not thoroughly determined. Here, we show that projecting to retrosplenial cortex are most active during synchronized cortical states such as non-rapid eye movement (NREM) sleep suppressed increased desynchronization arousal, movement, REM sleep. The claustrocortical signaling is NREM diminished due part cholinergic tone. Finally, activation enhances memory consolidation through phase resetting delta waves. Therefore, constrained function effectively cognitive processes consolidation.

Language: Английский

Citations

13
Claustral neurons projecting to frontal cortex restrict opioid consumption DOI Creative Commons

Anna Terem,

Yonatan Fatal,

Noa Peretz‐Rivlin

et al.

Current Biology, Journal Year: 2023, Volume and Issue: 33(13), P. 2761 - 2773.e8

Published: June 27, 2023

The synthetic opioid fentanyl is a major contributor to the current addiction crisis. We report that claustral neurons projecting frontal cortex limit oral self-administration in mice. found transcriptionally activates frontal-projecting claustrum neurons. These also exhibit unique suppression of Ca2+ activity upon initiation bouts consumption. Optogenetic stimulation neurons, intervening this suppression, decreased In contrast, constitutive inhibition context novel, group-housed procedure increased bout This same manipulation sensitized conditioned-place preference for and enhanced representation experience cortex. Together, our results indicate exert inhibitory control over cortical restrict intake. Upregulation claustro-frontal projection may be promising strategy reducing human addiction.

Language: Английский

Citations

11
Single-cell spatial transcriptome atlas and whole-brain connectivity of the macaque claustrum DOI Creative Commons
Ying Lei, Yuxuan Liu, Mingli Wang

et al.

Cell, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Claustrum orchestrates brain functions via its connections with numerous regions, but molecular and cellular organization remains unresolved. Single-nucleus RNA sequencing of 227,750 macaque claustral cells identified 48 transcriptome-defined cell types, most glutamatergic neurons similar to deep-layer insular neurons. Comparison macaque, marmoset, mouse transcriptomes revealed macaque-specific types. Retrograde tracer injections at 67 cortical 7 subcortical regions defined four distinct distribution zones retrogradely labeled Joint analysis whole-brain connectivity single-cell spatial transcriptome showed that these containing compositions (but not GABAergic) types preferentially connected specific a strong ipsilateral bias. Several in ventral vs. dorsal selectively co-projected two functionally related areas-entorhinal cortex hippocampus motor putamen, respectively. These data provide the basis for elucidating neuronal underlying diverse functions.

Language: Английский

Citations

0
Latexin and calretinin together define a novel excitatory neuron subclass in the claustrum of the short‐tailed fruit bat, Carollia perspicillata DOI
Timothy Morello,

Richard Kollmar,

Mark Stewart

et al.

Annals of the New York Academy of Sciences, Journal Year: 2025, Volume and Issue: unknown

Published: April 27, 2025

Abstract The claustrum is a telencephalic structure with inputs from and outputs to many other brain structures. This central arrangement has motivated research on the claustrum's role in cognition highlights need understand its intrinsic connectivity. In fruit bat, Carollia perspicillata , large size of offers access structure. Previously, we defined C. antibodies against latexin as an excitatory cell marker calcium‐binding proteins inhibitory markers. Using this immunohistochemical method, have now identified unexpected type concurrent calretinin immunoreactivity. + neurons claustrum, including those that coexpress GAD67 (another marker) latexin, are located claustral shell subregion. Neuronal /calretinin somata smaller than either − or somata. Since labels glutamatergic multiple areas never been found colocalize GAD, conclude excitatory. They represent one three types identifiable demonstrate can label both cells claustrum.

Language: Английский

Citations

0
Distinct 5-HT receptor subtypes regulate claustrum excitability by serotonin and the psychedelic, DOI DOI
Tanner L. Anderson,

Jack Keady,

Judy C. Songrady

et al.

Progress in Neurobiology, Journal Year: 2024, Volume and Issue: 240, P. 102660 - 102660

Published: Aug. 31, 2024

Language: Английский

Citations

3
Claustrum and dorsal endopiriform cortex complex cell-identity is determined by Nurr1 and regulates hallucinogenic-like states in mice DOI Creative Commons
Ioannis Mantas,

Ivana Flais,

Yuvarani Masarapu

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Sept. 18, 2024

Abstract The Claustrum/dorsal endopiriform cortex complex (CLA) is an enigmatic brain region with extensive glutamatergic projections to multiple cortical areas. transcription factor Nurr1 highly expressed in the CLA, but its role this not understood. By using conditional gene-targeted mice, we show that a crucial regulator of CLA neuron identity. Although neurons remain intact absence Nurr1, distinctive gene expression pattern abolished. has been hypothesized control hallucinations, little known how responds hallucinogens. After deletion both hallucinogen receptor and signaling are lost. Furthermore, functional ultrasound Neuropixel electrophysiological recordings revealed hallucinogenic-receptor agonists’ effects on connectivity between prefrontal sensorimotor cortices altered Nurr1-ablated mice. Our findings suggest Nurr1-targeted strategies provide additional avenues for studies CLA.

Language: Английский

Citations

3
Median raphe glutamatergic neuron-mediated enhancement of GABAergic transmission and suppression of long-term potentiation in the hippocampus DOI Creative Commons
Hannah E. Stinson, Ipe Ninan

Heliyon, Journal Year: 2024, Volume and Issue: 10(19), P. e38192 - e38192

Published: Sept. 20, 2024

The ascending neuromodulatory pathway from the median raphe nucleus (MRN) extends widely throughout midline/para-midline regions and robustly innervates hippocampus. This is believed to be critical for regulating emotional affective behaviors. Although MRN primarily contains serotoninergic (5-HTergic), GABAergic, glutamatergic neurons, neurons expressing vesicular glutamate transporter 3 (VGLUT3) form primary input Despite earlier demonstration of robust VGLUT3 innervation hippocampus, little known about how this modulates synaptic transmission plasticity in Our studies show that activate serotonin 3a receptor (5-HT3aR)-expressing GABAergic including VGLUT3-expressing at stratum radiatum (SR)/stratum lacunosum moleculare (SLM) border. neuron-mediated onto SR/SLM 5-HT3aR negatively regulated by 5-HT through 5-HT1B receptors. In agreement with activation projections induces a long-lasting increase but not CA1 pyramidal male female mice. Consistent enhancement mice, suppresses Schaffer collateral (SC)-CA1 long-term potentiation (LTP) Thus, our results modulate dorsal hippocampus augmenting inhibition suppressing SC-CA1 LTP sex-specific manner.

Language: Английский

Citations

3