Opposing effects of rewarding and aversive stimuli on D1 and D2 types of dopamine-sensitive neurons in the central amygdala DOI Creative Commons
Anna Beroun, Łukasz Bijoch,

Paweł Szczypkowski

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 17, 2024

Abstract Dopamine-sensitive neurons are organized in two classes of cells, expressing D1- or D2- types dopamine receptors, and often mediating opposing aspects reward-oriented behaviors. Here, we focused on dopamine-sensitive the central amygdala – a brain structure critically involved processing emotion-related stimuli. Exposing mice to rewarding aversive stimuli studied DRD1 DRD2 cells activity using in vivo two-photon calcium imaging CeM. We showed that cocaine sugar predominantly increase DRD1(+) decrease DRD2(+) cells. Repeated exposure cocaine, however, had opposite effect spontaneous excitatory synaptic transmission CeM than sugar. Quinine, an stimulus, primarily engaged neurons, activating those were previously inhibited by exposure. Our results show though populations differentially regulated appetitive/aversive stimuli, both participate sugar, quinine processing.

Language: Английский

The rostromedial tegmental nucleus gates fat overconsumption through ventral tegmental area output in male rats DOI

Florian Schoukroun,

Katia Befort,

Romain Bourdy

et al.

Neuropsychopharmacology, Journal Year: 2024, Volume and Issue: 49(10), P. 1569 - 1579

Published: April 3, 2024

Language: Английский

Citations

3
Opposing effects of rewarding and aversive stimuli on D1 and D2 types of dopamine-sensitive neurons in the central amygdala DOI Open Access
Łukasz Bijoch,

Paweł Szczypkowski,

Justyna Wiśniewska

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 7, 2024

Abstract Dopamine-sensitive neurons are organized in two classes of cells, expressing D1- or D2- types dopamine receptors, and often mediating opposing aspects reward-oriented behaviors. Here, we focused on dopamine-sensitive the central amygdala – a brain structure critically involved processing emotion-related stimuli. We discovered that both receptor present medial nucleus, while lateral part is populated predominantly with DRD2 cells. Exposing mice to rewarding aversive stimuli studied DRD1 cells activity using vivo two-photon calcium imaging CeM. showed cocaine sugar increase DRD1(+) decrease DRD2(+) Repeated exposure cocaine, however, had opposite effect spontaneous excitatory synaptic transmission CeM than sugar. Quinine, an stimulus, primarily engaged neurons, activating those were previously inhibited by exposure. Our results show though populations differentially regulated appetitive/aversive stimuli, participate sugar, quinine processing.

Language: Английский

Citations

1
EZH2-dependent epigenetic reprogramming in the central nucleus of amygdala regulates adult anxiety in both sexes after adolescent alcohol exposure DOI Creative Commons
John Bohnsack,

Huaibo Zhang,

Subhash C. Pandey

et al.

Translational Psychiatry, Journal Year: 2024, Volume and Issue: 14(1)

Published: April 26, 2024

Abstract Alcohol use and anxiety disorders occur in both males females, but despite sharing similar presentation classical symptoms, the prevalence of alcohol disorder (AUD) is lower females. While a symptom comorbidity shared by sexes, common underlying mechanism that leads to AUD subsequent development still understudied. Using rodent model adolescent intermittent ethanol (AIE) exposure we investigated epigenetic mediated enhancer zeste 2 (EZH2), histone methyltransferase, regulating expression activity-regulated cytoskeleton-associated protein (Arc) an anxiety-like phenotype adulthood. Here, report EZH2 levels were significantly higher PKC-δ positive GABAergic neurons central nucleus amygdala (CeA) adult male female rats after AIE. Reducing mRNA using siRNA infusion CeA prevented AIE-induced behavior, increased H3K27me3, decreased H3K27ac at Arc synaptic activity response element (SARE) site, restored deficits rats. Our data indicate EZH2-mediated plays important role behavior AIE This study suggests may serve as tractable drug target for treatment psychopathology exposure.

Language: Английский

Citations

0
Opposing effects of rewarding and aversive stimuli on D1 and D2 types of dopamine-sensitive neurons in the central amygdala DOI Creative Commons
Anna Beroun, Łukasz Bijoch,

Paweł Szczypkowski

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 17, 2024

Abstract Dopamine-sensitive neurons are organized in two classes of cells, expressing D1- or D2- types dopamine receptors, and often mediating opposing aspects reward-oriented behaviors. Here, we focused on dopamine-sensitive the central amygdala – a brain structure critically involved processing emotion-related stimuli. Exposing mice to rewarding aversive stimuli studied DRD1 DRD2 cells activity using in vivo two-photon calcium imaging CeM. We showed that cocaine sugar predominantly increase DRD1(+) decrease DRD2(+) cells. Repeated exposure cocaine, however, had opposite effect spontaneous excitatory synaptic transmission CeM than sugar. Quinine, an stimulus, primarily engaged neurons, activating those were previously inhibited by exposure. Our results show though populations differentially regulated appetitive/aversive stimuli, both participate sugar, quinine processing.

Language: Английский

Citations

0