Parvalbumin
(PV)-positive
interneurons
modulate
the
processing
of
odor
information.
However,
less
is
known
about
how
PV
dynamically
remodel
neural
circuit
responses
in
olfactory
bulb
(OB)
and
its
physiological
significance.
This
study
showed
that
a
reinforced
discrimination
task
up-regulated
activity
ErbB4
kinase
mouse
OB.
knock-out
OB
impaired
dishabituation
complex
odors,
whereas
memory
or
adaptation
had
no
alteration
mice.
RNAscope
analysis
demonstrated
ErbB4-positive
neurons
are
localized
throughout
OB,
within
internal
external
plexiform
layers,
mRNA
largely
expressed
PV-positive
interneurons.
disrupted
odor-evoked
mitral/tufted
cells,
led
to
increased
power
ongoing
local
field
potential
awake
We
also
found
decrease
frequency
miniature
inhibitory
postsynaptic
currents
deficits
stimulus-evoked
recurrent
lateral
inhibition
onto
mitral
suggesting
broad
impairments
microcircuit
following
PV-ErbB4
loss.
Similarly,
ablation
These
findings
provide
novel
insights
into
role
signaling
plasticity,
oscillations,
output,
which
underlies
normal
behaviors.
Current Opinion in Genetics & Development,
Journal Year:
2024,
Volume and Issue:
88, P. 102236 - 102236
Published: Aug. 16, 2024
The
human
brain
has
evolved
unique
capabilities
compared
to
other
vertebrates.
mechanistic
basis
of
these
derived
traits
remains
a
fundamental
question
in
biology
due
its
relevance
the
origin
our
cognitive
abilities
and
behavioral
repertoire,
as
well
human-specific
aspects
neuropsychiatric
neurodegenerative
diseases.
Comparisons
those
nonhuman
primates
mammals
have
revealed
that
differences
neuromodulatory
systems,
especially
dopaminergic
system,
may
govern
some
alterations,
including
increased
vulnerability
certain
disorders.
In
this
review,
we
highlight
discuss
recent
findings
human-
primate-specific
alterations
focusing
on
anatomy,
circuitry,
molecular
properties.
Neuronal
function
is
intimately
tied
to
axodendritic
polarity.
Neurotransmitter
release,
for
example,
usually
the
role
of
axon.
There
are
widespread
exceptions
this
rule,
however,
including
many
mammalian
neuronal
types
that
can
release
neurotransmitter
from
their
dendrites.
In
mouse
olfactory
bulb,
closely
related
subclasses
dopaminergic
interneuron
differ
markedly
in
polarity,
with
one
subtype
lacking
an
axon
entirely.
These
axon-bearing
and
anaxonic
have
distinct
developmental
profiles
sensory
responses,
but
how
fundamental
polarity
differences
translate
functional
outputs
remains
entirely
unknown.
Here,
we
provide
anatomical
evidence
strategies
among
these
subtypes:
cells
dendrites,
while
neurons
exclusively
intermittently
myelinated
structural
linked
a
clear
distinction:
anaxonic,
not
capable
self-inhibition.
Our
findings
suggest
variations
produce
striking
distinctions
outputs,
even
may
play
separate
roles
information
processing.
Neuronal
function
is
intimately
tied
to
axodendritic
polarity.
Neurotransmitter
release,
for
example,
usually
the
role
of
axon.
There
are
widespread
exceptions
this
rule,
however,
including
many
mammalian
neuronal
types
that
can
release
neurotransmitter
from
their
dendrites.
In
mouse
olfactory
bulb,
closely
related
subclasses
dopaminergic
interneuron
differ
markedly
in
polarity,
with
one
subtype
lacking
an
axon
entirely.
These
axon-bearing
and
anaxonic
have
distinct
developmental
profiles
sensory
responses,
but
how
fundamental
polarity
differences
translate
functional
outputs
remains
entirely
unknown.
Here,
we
provide
anatomical
evidence
strategies
among
these
subtypes:
cells
dendrites,
while
neurons
exclusively
intermittently
myelinated
structural
linked
a
clear
distinction:
anaxonic,
not
capable
self-inhibition.
Our
findings
suggest
variations
produce
striking
distinctions
outputs,
even
may
play
separate
roles
information
processing.
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 25, 2025
All
optical
physiology
in
vivo
provides
a
conduit
for
investigating
the
function
of
neural
circuits
3-D.
Here,
we
report
new
strategy
flexible,
axially-decoupled
photo-stimulation
and
two
photon
readout
(ADePT)
neuronal
activity.
To
achieve
axially-contained
widefield
optogenetic
patterned
stimulation,
couple
digital
micro-mirror
device
illuminated
by
solid-state
laser
with
motorized
holographic
diffuser.
In
parallel,
use
multiphoton
imaging
activity
across
different
z-planes.
We
ADePT
to
analyze
excitatory
inhibitory
functional
connectivity
mouse
early
olfactory
system.
Specifically,
control
individual
input
glomeruli
on
bulb
surface,
map
ensuing
responses
output
mitral
tufted
cell
bodies
deeper
layers.
This
approach
identifies
cohorts
sister
cells,
whose
firing
is
driven
same
parent
glomerulus,
also
reveals
their
differential
inhibition
other
glomeruli.
addition,
selective
activation
glomerular
GABAergic/dopaminergic
(DAT+)
interneurons
triggers
dense,
but
spatially
heterogeneous
suppression
baseline
odor
responses,
further
demonstrating
specificity
connectivity.
summary,
enables
high-throughput
mapping
optically
accessible
brain
regions.
The Journal of Physiology,
Journal Year:
2024,
Volume and Issue:
602(14), P. 3519 - 3543
Published: June 5, 2024
In
mammals,
odour
information
within
the
olfactory
bulb
(OB)
is
processed
by
complex
neural
circuits
before
being
ultimately
represented
in
action
potential
activity
of
mitral/tufted
cells
(M/Ts).
Cholecystokinin-expressing
(CCK
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2024,
Volume and Issue:
unknown
Published: July 25, 2024
Abstract
Parvalbumin
(PV)-positive
interneurons
modulate
the
processing
of
odor
information.
However,
less
is
known
about
how
PV
dynamically
remodel
neural
circuit
responses
in
olfactory
bulb
(OB)
and
its
physiological
significance.
This
study
showed
that
a
reinforced
discrimination
task
up-regulated
activity
ErbB4
kinase
mouse
OB.
knock-out
OB
impaired
dishabituation
complex
odors,
whereas
memory
or
adaptation
had
no
alteration
mice.
RNAscope
analysis
demonstrated
ErbB4-positive
neurons
are
localized
throughout
OB,
within
internal
external
plexiform
layers,
mRNA
largely
expressed
PV-positive
interneurons.
disrupted
odor-evoked
mitral/tufted
cells,
led
to
increased
power
ongoing
local
field
potential
awake
We
also
found
decrease
frequency
miniature
inhibitory
postsynaptic
currents
deficits
stimulus-evoked
recurrent
lateral
inhibition
onto
mitral
suggesting
broad
impairments
microcircuit
following
PV-ErbB4
loss.
Similarly,
ablation
These
findings
provide
novel
insights
into
role
signaling
plasticity,
oscillations,
output,
which
underlies
normal
behaviors.
Journal of Neurophysiology,
Journal Year:
2024,
Volume and Issue:
132(3), P. 943 - 952
Published: Aug. 7, 2024
Cotransmission,
meaning
the
release
of
multiple
neurotransmitters
from
one
synapse,
allows
for
increased
diversity
signaling
in
brain.
Dopamine
(DA)
and
γ-aminobutyric
acid
(GABA)
are
known
to
coexpress
many
regions
such
as
olfactory
bulb
ventral
tegmental
area.
Tuberoinfundibular
dopaminergic
neurons
(TIDA)
arcuate
nucleus
hypothalamus
(Arc)
project
median
eminence
(ME)
regulate
prolactin
pituitary,
prior
work
suggests
Arc
also
cotransmit
GABA.
However,
extent
cotransmission,
projection
patterns
these
have
not
been
fully
revealed.
Here,
we
used
a
genetic
intersectional
reporter
expression
approach
selectively
label
cells
that
express
both
tyrosine
hydroxylase
(TH)
vesicular
GABA
transporter
(VGAT).
Through
this
approach,
identified
capable
DA
cotransmission
Arc,
periventricular
(Pe),
paraventricular
(Pa),
ventromedial,
dorsolateral
hypothalamic
nuclei,
addition
novel
population
caudate
putamen.
The
highest
density
labeled
was
6.68%
DAPI-labeled
at
Bregma
-2.06
mm,
Pe,
2.83%
-1.94
mm.
Next,
evaluated
projections
DA/GABA
by
injecting
an
mCherry
virus
fluoresces
cells.
We
observed
cotransmitting
population,
with
within
Pa
ME.
These
data
suggest
involved
subset
TIDA
neurons.
Further
investigation
will
elucidate
interactions
dopamine
hypothalamus.
