Protocol to generate a microfluidic vessels-on-chip platform using human pluripotent stem cell-derived endothelial cells DOI Creative Commons

Caroline Remmert,

Munkhtur Otgonbayar,

Julius Alexander Perschel

et al.

STAR Protocols, Journal Year: 2024, Volume and Issue: 5(3), P. 103300 - 103300

Published: Sept. 1, 2024

Here, we present a protocol for producing microfluidic vessel-on-chip platform using human pluripotent stem cell-derived endothelial cells (SC-ECs). We describe steps manufacturing the 3D-printed chip, cell culturing to generate SC-ECs, hydrogel patterning, and formation cultivation of barrier-forming vessels. then detail procedures retrieval media from open chip enable multi-omics analysis. For complete details on use execution this protocol, please refer Marder et al.

Language: Английский

Innovative Atherosclerosis Models: Advancing Pathophysiology and Translational Research DOI Creative Commons

Huiting Jiang,

Yukun Liao,

Mengliang Zhu

et al.

Research, Journal Year: 2025, Volume and Issue: 8

Published: Jan. 1, 2025

Atherosclerosis (AS) is a chronic inflammatory condition influenced by glucose and lipid disorders, oxidative stress, thrombosis, reflecting the complexity of its pathological process. The development accurate experimental models that simulate human AS essential for understanding initiation progression. This review summarizes current research analyzes their specific application scenarios. We discuss tissue-engineered blood vessels (TEBVs) vessels-on-a-chip (VoCs), which leverage tissue engineering precise microenvironmental control to construct in vitro closely resemble structure function AS. Isolated vessel segments from live animals provide valuable tool investigating due physiological similarity, controllability, reproducibility. further outlines construction animal through high-fat diets gene-editing techniques, highlighting how immune-inflammatory responses, mechanical arterial injury, hemodynamic changes accelerate model development. comprehensive analysis highlights potential revolutionize theranostic applications clinical translational research, paving way more personalized effective treatments near future.

Language: Английский

Citations

1
Human Vascularized Adipose Tissue Organoids Reveal Endothelial-Adipocyte Interactions at Single-Cell Resolution DOI

Maren Marder,

Sandra Wiedenmann, Fabiana Oliveira

et al.

Published: Jan. 1, 2025

White adipose tissue (WAT) is essential for energy storage and metabolic homeostasis, plays a central role in the pathology of obesity type 2 diabetes. Vascular networks within WAT support oxygen nutrient supply regulate adipogenesis, yet mechanisms underlying endothelial-adipocyte crosstalk under both healthy dysfunctional conditions remain unclear. Here, we present vascularized organoid (vAO) model engineered from human adipose-derived stem cells induced pluripotent cell-derived endothelial (SC-ECs). The 3D cell culture enables adipogenesis angiogenesis controlled conditions. vAOs demonstrated enhanced β-adrenergic-induced lipolysis insulin-stimulated glucose uptake. Single-cell transcriptomics revealed distinct stromal subtypes, including perivascular adipocyte precursors maturing SC-ECs shaped by PDGFB signaling. knockdown disrupted alignment lipolysis, highlighting critical endothelial-pericyte interactions. This study provides versatile platform to dissect explore pathways that control WAT-remodeling determine its function. Our vAO therefore presents unique molecularly functionally patient-specific dysfunctions, advancing our portfolio personalized therapies importance communication.

Language: Английский

Citations

0
Towards advanced regenerative therapeutics to tackle cardio-cerebrovascular diseases DOI Creative Commons
Xi Chen,

Weiping Lin,

Micky D. Tortorella

et al.

American Heart Journal Plus Cardiology Research and Practice, Journal Year: 2025, Volume and Issue: 53, P. 100520 - 100520

Published: March 1, 2025

The development of vascularized organoids as novel modelling tools the human cardio-cerebrovascular system for preclinical research has become an essential platform studying tissues/organs personalized therapeutics during recent decades. Organ-on-chip technology is promising investigating physiological in vitro responses drug screening and advanced disease models. Vascularized tissue/organ-on-a-chip benefits every step discovery pipeline a tool with close genome relevance to investigate systems biology. Simultaneously, cardio-cerebrovascular-on-chip-integrated microfluidic serves alternative animal (patho-)physiological processes blood vessels embryonic disease. Integrated next-generation techniques, such three-dimensional bioprinting both cells matrix, may enable organoid-on-chip-based therapeutics.

Language: Английский

Citations

0
Artery regeneration: Molecules, mechanisms and impact on organ function DOI
Swarnadip Ghosh,

Bhavnesh Bishnoi,

Soumyashree Das

et al.

Seminars in Cell and Developmental Biology, Journal Year: 2025, Volume and Issue: 171, P. 103611 - 103611

Published: May 2, 2025

Language: Английский

Citations

0
Recent Advances and Future Perspectives in Vascular Organoids and Vessel-on-Chip DOI Creative Commons

Gowtham Reddy Cheruku,

Chloe Veronica Wilson,

Suriya Raviendran

et al.

Organoids, Journal Year: 2024, Volume and Issue: 3(3), P. 203 - 246

Published: Sept. 4, 2024

Recent advancements in vascular organoid (VO) and vessel-on-chip (VoC) technologies have revolutionized our approach to studying human diseases, offering unprecedented insights through more physiologically relevant models. VOs generated from pluripotent stem cells exhibit remarkable self-organization capabilities, forming complex three-dimensional structures that closely mimic blood vessel architecture function, while VoCs are engineered with microfluidic systems meticulously recreate the physical functional attributes of vessels. These innovative constructs serve as powerful tools for investigating development, disease progression, therapeutic efficacy. By enabling creation patient-specific VoCs, they pave way personalized medicine approaches, allowing researchers delve into genetic variations, intricate cellular interactions, dynamic processes exceptional resolution. The synergy between newly developed cutting-edge has further amplified their potential, unveiling novel mechanisms underlying pathologies identifying promising targets. Herein, we summarize different types present an extensive overview on generation applications VoCs. We will also highlight clinical translational challenges future perspectives around

Language: Английский

Citations

2
Recent Advances and Future Perspectives in Vascular Organoids and Vessel-on-Chip DOI Open Access

Gowtham Reddy Cheruku,

Chloe Veronica Wilson,

Suriya Raviendran

et al.

Published: July 14, 2024

Recent advancements in vascular organoid (VO) and vessel-on-chip (VoC) technologies have revolutionized our approach to studying cardiovascular diseases (CVDs), offering unprecedented insights through more physiologically relevant models. VOs generated from human pluripotent stem cells exhibit remarkable self-organization capabilities, forming complex three-dimensional structures that closely mimic blood vessel architecture function, while VoCs engineered with groundbreaking microfluidic systems meticulously recreate the physical functional attributes of vessels. These innovative constructs serve as powerful tools for investigating development, disease progression, therapeutic efficacy. By enabling creation patient-specific VoCs, they pave way personalized medicine approaches, allowing researchers delve into genetic variations, intricate cellular interactions, dynamic processes exceptional resolution. The synergy between cutting-edge such single-cell sequencing high-resolution imaging has further amplified their potential, unveiling novel mechanisms underlying CVDs identifying promising targets. Herein, we summarize different types present an extensive overview on generation applications CVDs. We will also highlight clinical translational challenges future perspectives around VoCs.

Language: Английский

Citations

1
Protocol to generate a microfluidic vessels-on-chip platform using human pluripotent stem cell-derived endothelial cells DOI Creative Commons

Caroline Remmert,

Munkhtur Otgonbayar,

Julius Alexander Perschel

et al.

STAR Protocols, Journal Year: 2024, Volume and Issue: 5(3), P. 103300 - 103300

Published: Sept. 1, 2024

Here, we present a protocol for producing microfluidic vessel-on-chip platform using human pluripotent stem cell-derived endothelial cells (SC-ECs). We describe steps manufacturing the 3D-printed chip, cell culturing to generate SC-ECs, hydrogel patterning, and formation cultivation of barrier-forming vessels. then detail procedures retrieval media from open chip enable multi-omics analysis. For complete details on use execution this protocol, please refer Marder et al.

Language: Английский

Citations

1