GsMTx-4 venom toxin antagonizes biophysical modulation of metastatic traits in human osteosarcoma cells DOI Creative Commons

Arianna Buglione,

Giulia Alloisio, Chiara Ciaccio

et al.

European Journal of Cell Biology, Journal Year: 2024, Volume and Issue: 104(1), P. 151469 - 151469

Published: Dec. 11, 2024

Despite their genetic diversity, metastatic cells converge on similar physical constraints during tumor progression. At the nanoscale, these forces can induce substantial molecular deformations, altering structure and behavior of cancer cells. To address challenges osteosarcoma (OS), a highly aggressive cancer, we explored mechanobiology OS cells, in vitro. Using uniaxial-stretching technology, examined biophysical modulation traits SAOS-2, U-2 OS, non-tumorigenic hFOB Changes cell morphology were quantified using confocal fluorescence microscopy. elucidate mechanisms that translate biomechanical alterations into biochemical responses, employed Western blotting, real-time quantitative RT-PCR, reactive oxygen species ROS assay, mechanosensitive channel blocker Grammostola MechanoToxin4 (GsMTx-4). Our study reveals mechanical stimulation uniquely affects increasing nuclear size N/C ratio. We found (MS) channels are activated, leading to accumulation, Src protein modulation, histone H3 acetylation. These changes influence motility adhesion but not proliferation. Importantly, preconditioning differentially impacts doxorubicin resistance, correlating with Src-H3 acetylation axis. This underscores critical role MS highlights importance identifying pathways traditional approaches may reveal. Notably, GsMTx-4 venom peptide effectively countered mechanically induced particularly by inhibiting migration, without harming healthy Thus, suggesting its potential as promising therapeutic agent for targeting metastasis.

Language: Английский

Mechano‐Responsive Biomaterials for Bone Organoid Construction DOI Creative Commons
Ruiyang Li, Jian Wang,

Qiushui Lin

et al.

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 30, 2024

Abstract Mechanical force is essential for bone development, homeostasis, and fracture healing. In the past few decades, various biomaterials have been developed to provide mechanical signals that mimic natural microenvironment, thereby promoting regeneration. Bone organoids, emerging as a novel research approach, are 3D micro‐bone tissues possess ability self‐renew self‐organize, exhibiting biomimetic spatial characteristics. Incorporating mechano‐responsive in construction of organoids presents promising avenue simulating microenvironment. Therefore, this review commences by elucidating impact on health, encompassing both cellular interactions alterations structure. Furthermore, most recent applications within realm tissue engineering highlighted. Three different types introduced with focus their responsive mechanisms, strategies, efficacy facilitating Based comprehensive overview, prospective utilization future challenges discussed. As organoid technology advances, these poised become powerful tools

Language: Английский

Citations

5
Biomechanics and Mechanobiology of Additively Manufactured Porous Load‐Bearing Bone Implants DOI
Yuan Jin, Jianhui Li,

Haitao Fan

et al.

Small, Journal Year: 2025, Volume and Issue: unknown

Published: April 17, 2025

Abstract Given that they can replicate both the biomechanical and mechanobiological functions of natural bone, metal additively manufactured porous load‐bearing bone implants present a significant advancement in orthopedic applications. Additive manufacturing (AM) metals enables precise control over pore geometry, resulting provide effective mechanical support minimize stress shielding. In addition to its benefits, architecture facilitates essential processes, including transmission signals regulate cellular processes such as adhesion, proliferation, differentiation. Before clinical use, should first be engineered achieve comparable elastic modulus native mitigating implant‐induced resorption while promoting tissue regeneration. It is also noteworthy microstructural features these angiogenesis‐a critical process for oxygen nutrient delivery during healing. Despite their potential challenges remain balancing stability applications with biofunctionality integration controlled degradation. This review comprehensively discusses factors influencing design performance implants, highlighting enhance outcomes repair

Language: Английский

Citations

0
Annexin A family: A new perspective on the regulation of bone metabolism DOI Open Access
Ke Xu, Ruiqi Huang, Ruiming Wen

et al.

Biomedicine & Pharmacotherapy, Journal Year: 2024, Volume and Issue: 178, P. 117271 - 117271

Published: Aug. 8, 2024

Osteoblast-mediated bone formation and osteoclast-mediated resorption are critical processes in metabolism. Annexin A, a calcium-phospholipid binding protein, regulates the proliferation differentiation of cells, including marrow mesenchymal stem osteoblasts, osteoclasts, has gradually become marker gene for diagnosis osteoporosis. As calcium channel proteins, annexin A family members closely associated with mechanical stress, which can target annexins A1, A5, A6 to promote cell differentiation. Despite significant clinical potential metabolism, few studies have reported on these mechanisms. Therefore, based review relevant literature, this article elaborates specific functions possible mechanisms metabolism provide new ideas their application prevention treatment diseases, such as

Language: Английский

Citations

1
GsMTx-4 venom toxin antagonizes biophysical modulation of metastatic traits in human osteosarcoma cells DOI Creative Commons

Arianna Buglione,

Giulia Alloisio, Chiara Ciaccio

et al.

European Journal of Cell Biology, Journal Year: 2024, Volume and Issue: 104(1), P. 151469 - 151469

Published: Dec. 11, 2024

Despite their genetic diversity, metastatic cells converge on similar physical constraints during tumor progression. At the nanoscale, these forces can induce substantial molecular deformations, altering structure and behavior of cancer cells. To address challenges osteosarcoma (OS), a highly aggressive cancer, we explored mechanobiology OS cells, in vitro. Using uniaxial-stretching technology, examined biophysical modulation traits SAOS-2, U-2 OS, non-tumorigenic hFOB Changes cell morphology were quantified using confocal fluorescence microscopy. elucidate mechanisms that translate biomechanical alterations into biochemical responses, employed Western blotting, real-time quantitative RT-PCR, reactive oxygen species ROS assay, mechanosensitive channel blocker Grammostola MechanoToxin4 (GsMTx-4). Our study reveals mechanical stimulation uniquely affects increasing nuclear size N/C ratio. We found (MS) channels are activated, leading to accumulation, Src protein modulation, histone H3 acetylation. These changes influence motility adhesion but not proliferation. Importantly, preconditioning differentially impacts doxorubicin resistance, correlating with Src-H3 acetylation axis. This underscores critical role MS highlights importance identifying pathways traditional approaches may reveal. Notably, GsMTx-4 venom peptide effectively countered mechanically induced particularly by inhibiting migration, without harming healthy Thus, suggesting its potential as promising therapeutic agent for targeting metastasis.

Language: Английский

Citations

1