Lmx1a is essential for marginal cell differentiation and stria vascularis formation

Frontiers in Cell and Developmental Biology, Journal Year: 2025, Volume and Issue: 13

Published: March 5, 2025

The transcription factor Lmx1a is widely expressed during early inner ear development, and mice lacking expression exhibit fusion of cochlear vestibular hair cells fail to form the ductus reuniens endolymphatic sac. dreher (Lmx1a dr/dr ), a recessive null mutation, results in non-functional expression, which expands from outer sulcus stria vascularis Reissner's membrane. In absence Lmx1a, we observe lack proteins specific vascularis, such as BSND KCNQ1 marginal CD44 intermediate cells. Further analysis superficial epithelial cell layer at expected location shows that future migrate embryonic development but subsequently disappear. Using antibodies against pendrin (Slc26a4) knockout (KO) mice, an expansion across Moreover, no endocochlear potential observed. These findings highlight critical role particularly differentiation structures, recruitment pigment cells, essential for hearing balance.

Language: Английский

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Expression of S100β during mouse cochlear development

European Journal of Histochemistry, Journal Year: 2025, Volume and Issue: 69(1)

Published: March 10, 2025

In the present study, expression of S100β was examined in mouse cochlea from embryonic day 17 (E17) to postnatal 32 (P32) using immunofluorescence, aiming explore its possible role auditory system. At E17, not detected, except external cochlear wall. Starting at E18.5, staining appeared organ Corti and stria vascularis. E18.5 P1 Corti, confined developing pillar cells. By P6, cytoplasmic evident inner outer cells, forming tunnel Corti. Additionally, extended medially into three rows Deiter’s with labeling their phalangeal processes. P8, continued be expressed heads, bodies, feet two as well soma processes lateral wall P8 cochlea, only vascularis but also spiral ligament. Between P10 P12, maintained cells wall, limbus. From P14 onwards, ceased vascularis, though it persisted ligament limbus adulthood. Within P21 remained immunostaining observed specifically cell cups P21. adult (P28 P32), declined both The dynamic spatiotemporal changes during ontogeny suggest development hearing function.

Language: Английский

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Conditional Overexpression of Serpine2 Promotes Hair Cell Regeneration from Lgr5+ Progenitors in the Neonatal Mouse Cochlea

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: March 17, 2025

Abstract Neonatal cochlear Lgr5+ progenitors retain limited hair cells (HCs) regenerative capacity, but the regulatory network remains incompletely defined. Serpin family E member 2 ( Serpine2 ) is shown to participate in regulating proliferation and differentiation of previous vitro study. Here, expression pattern vivo roles HC regeneration are explored by transgenic mice. It found that expressed mouse cochlea after birth with a downward trend as mice age. In addition, conditional overexpression neonatal results an increased number ectopic HCs dose‐dependent manner. knockdown ex can inhibit regeneration. EdU assay lineage tracing demonstrate these likely originate from through direct transdifferentiation rather than mitotic Moreover, single‐nucleus RNA sequencing analysis mRNA level validation reveal conditionally overexpressed induces via inhibiting sonic hedgehog (SHH) signal pathway inducing Atoh1 Pou4f3 transcription factor. brief, data indicate plays pivotal role cochlea, this suggests new avenue for future research into

Language: Английский

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Multifaceted roles of sonic hedgehog signaling in mammalian inner ear development

Developmental Biology, Journal Year: 2025, Volume and Issue: unknown

Published: May 1, 2025

Language: Английский

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