An antibacterial, antioxidant and hemostatic hydrogel accelerates infectious wound healing DOI Creative Commons
Ziyi Zhou, Dengjun Zhang, Xuchao Ning

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 12, 2024

Abstract Hydrogel drug-delivery system that can effectively load antibacterial drugs, realize the in-situ drug release in microenvironment of wound infection to promote healing. In this study, a multifunctional hydrogel delivery (HA@TA-Okra) was constructed through integration hyaluronic acid methacrylate (HAMA) matrix with tannic (TA) and okra extract. The composition structural characteristics HA@TA-Okra its unique advantages treatment diverse wounds were systematically evaluated. TA, due chemical structure, is able anchor within HAMA network interactions cross-linking, conferring exceptional mechanical strength stability hydrogel. Both TA extract possess antioxidant properties, when they two acts synergistically scavenge free radicals, enhance action, diminishing risk infection. In vitro experiments revealed has superior such as rapid gel response, remarkable swelling regulation, potent ability. Furthermore, significantly outperformed conventional dressings terms hemostatic performance rat hemorrhage model. We further evaluated repair role in vivo by establishing an animal model full-thickness skin defects infected total defects. results confirmed positive effects fighting bacterial infection, reducing inflammation promoting summary, exhibits comprehensive properties antibacterial, which potential application field tissue medicine.

Language: Английский

An antibacterial, antioxidant and hemostatic hydrogel accelerates infectious wound healing DOI Creative Commons
Ziyi Zhou, Dengjun Zhang, Xuchao Ning

et al.

Journal of Nanobiotechnology, Journal Year: 2025, Volume and Issue: 23(1)

Published: Jan. 28, 2025

Abstract Hydrogel drug-delivery system that can effectively load antibacterial drugs, realize the in-situ drug release in microenvironment of wound infection to promote healing. In this study, a multifunctional hydrogel delivery (HA@TA-Okra) was constructed through integration hyaluronic acid methacrylate (HAMA) matrix with tannic (TA) and okra extract. The composition structural characteristics HA@TA-Okra its unique advantages treatment diverse wounds were systematically evaluated. TA, due chemical structure, is able anchor within HAMA network interactions cross-linking, conferring exceptional mechanical strength stability hydrogel. Both TA extract possess antioxidant properties, when they two acts synergistically scavenge free radicals, enhance action, diminishing risk infection. vitro experiments revealed has superior such as rapid gel response, remarkable swelling regulation, potent ability. Furthermore, significantly outperformed conventional dressings terms hemostatic performance rat hemorrhage model. We further evaluated repair role vivo by establishing an animal model full-thickness skin defects infected total defects. results confirmed positive effects fighting bacterial infection, reducing inflammation promoting summary, exhibits comprehensive properties antibacterial, which potential application field tissue medicine. Graphical

Language: Английский

Citations

2
Acute hypoxia modulate macrophage phenotype accompanied with transcriptome re-programming and metabolic re-modeling DOI Creative Commons
Binda Sun, Yao Long, Gang Xu

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 17, 2025

Introduction Macrophages, which tend to aggregate in the hypoxic regions of tissues, have a significant impact on disease progression and outcome because their plastic responsiveness hypoxia, particularly early stages. Understanding macrophages’participation hypoxia-related disorders requires demonstrating acute hypoxia survival, phenotype, function. Methods Here we conducted systematic evaluation macrophage responses over 24 48 h including cell growth activity, inflamatory response, polarization transcriptional metabolic changes. Results We found that suppresses proliferation phagocytosis function with parallel change transcriptome re-programming re-modeling. Although macrophages accumulate heterogeneity based oxygen concentration culture period, genes involved chemotaxis, glycolytic process were commonly altered during hypoxia. Furthermore, pro-inflammatory response was activated concomitantly an enhanced anti-inflammatory regulatory mechanism characterized by increased M2 population metabolite itaconic acid. Aside from glycolysis, key intermediates pentose phosphate pathway significantly increased, such as fructose 1,6-bisphosphate (fold change: 7.8), 6-phosphogluconate 6.1), ribose 5-phosphate 3.9), indicated important compensatory regulation rules for Discussion These findings highlight viability phagocytosis, while modifies metabolome specific inflammatory pathways facilitate adaptation conditions.

Language: Английский

Citations

0
Inflammation and cancer: molecular mechanisms and clinical consequences DOI Creative Commons
Hikmet Akkız, Cem Şimşek, Deniz Balcı

et al.

Frontiers in Oncology, Journal Year: 2025, Volume and Issue: 15

Published: March 17, 2025

Inflammation, a hallmark of cancer, has been associated with tumor progression, transition into malignant phenotype and efficacy anticancer treatments in cancer. It affects all stages from the initiation carcinogenesis to metastasis. Chronic inflammation induces immunosup-pression, providing an environment conducive carcinogenesis, whereas acute antitumor immune response, leading suppression. Solid tumors have inflammatory microenvironment (TME) containing cancer cells, stromal soluble molecules, which plays key role progression therapy response. Both cells TME are highly plastic constantly change their phenotypic functional properties. Cancer-associated inflammation, majority consists innate important cell plasticity, development drug resistance. Today, combined used advanced technologies, such as single-cell RNA sequencing spatial molecular imaging analysis, pathways linking chronic largely elucidated. In this review article, we highlighted cellular mechanisms involved cancer-associated its effects on treatment We also comprehensively setting GI cancers.

Language: Английский

Citations

0
Direct microglia replacement reveals pathologic and therapeutic contributions of brain macrophages to a monogenic neurological disease DOI
William H. Aisenberg, Carleigh A. O’Brien, Madison Sangster

et al.

Immunity, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

Citations

0
MORPHOFUNCTIONAL CHARACTERISTICS OF MESENTERIC LYMPH NODES IN THE COMBINED EFFECT OF HYPOXIA AND INFECTION DOI

S. M. Yagubova,

Tarana Nadir Mirzayeva,

T. S. Sultanova

et al.

World of Medicine and Biology, Journal Year: 2025, Volume and Issue: 21(91), P. 207 - 207

Published: Jan. 1, 2025

Language: Английский

Citations

0
HIF-1 regulates mitochondrial function in bone marrow-derived macrophages but not in tissue-resident alveolar macrophages DOI Creative Commons
P.S. Woods, Rengül Cetin‐Atalay,

Angelo Y. Meliton

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: April 4, 2025

Language: Английский

Citations

0
HIF-1α regulates mitochondrial function in bone marrow-derived macrophages, but not in tissue-resident alveolar macrophages DOI Creative Commons
P.S. Woods, Rengül Cetin‐Atalay,

Angelo Y. Meliton

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 15, 2024

Summary HIF-1α plays a critical role in shaping macrophage phenotype and effector function. We have previously shown that tissue-resident alveolar macrophages (TR-AMs) extremely low glycolytic capacity at steady-state, but can shift toward glycolysis under hypoxic conditions. Here, using inducible knockout ( Hif1a -/- ) TR-AMs bone marrow-derived (BMDMs) show TR-AM is required for the prolyl hydroxylase inhibition, dispensable steady-state inflammatory In contrast, deletion BMDMs led to diminished reduced capacity, higher mitochondrial Gene set enrichment analysis revealed enhanced c-Myc transcriptional activity BMDMs, upregulation of gene pathways related ribosomal biogenesis cellular proliferation. The findings highlight heterogeneity function distinct populations provide new insight into how regulates expression, inflammation, metabolism macrophages.

Language: Английский

Citations

2
An antibacterial, antioxidant and hemostatic hydrogel accelerates infectious wound healing DOI Creative Commons
Ziyi Zhou, Dengjun Zhang, Xuchao Ning

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 12, 2024

Abstract Hydrogel drug-delivery system that can effectively load antibacterial drugs, realize the in-situ drug release in microenvironment of wound infection to promote healing. In this study, a multifunctional hydrogel delivery (HA@TA-Okra) was constructed through integration hyaluronic acid methacrylate (HAMA) matrix with tannic (TA) and okra extract. The composition structural characteristics HA@TA-Okra its unique advantages treatment diverse wounds were systematically evaluated. TA, due chemical structure, is able anchor within HAMA network interactions cross-linking, conferring exceptional mechanical strength stability hydrogel. Both TA extract possess antioxidant properties, when they two acts synergistically scavenge free radicals, enhance action, diminishing risk infection. In vitro experiments revealed has superior such as rapid gel response, remarkable swelling regulation, potent ability. Furthermore, significantly outperformed conventional dressings terms hemostatic performance rat hemorrhage model. We further evaluated repair role in vivo by establishing an animal model full-thickness skin defects infected total defects. results confirmed positive effects fighting bacterial infection, reducing inflammation promoting summary, exhibits comprehensive properties antibacterial, which potential application field tissue medicine.

Language: Английский

Citations

0