Electroconvulsive therapy generates a hidden wave after seizure DOI Creative Commons
Zachary P. Rosenthal, Joseph B. Majeski, Ala Somarowthu

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 1, 2024

Abstract Electroconvulsive therapy (ECT) is a fast-acting, highly effective, and safe treatment for medication-resistant depression. Historically, the clinical benefits of ECT have been attributed to generating controlled seizure; however, underlying neurobiology understudied remains largely unresolved. Using optical neuroimaging probe neural activity hemodynamics in mouse model ECT, we demonstrated that second brain event follows seizure: cortical spreading depolarization (CSD). We further found stimulation pulse parameters electrode configuration directly shaped wave dynamics seizure subsequent CSD. To translate these findings human patients, tested presence hemodynamic signatures post-ictal CSD using non-invasive diffuse monitoring cerebral blood flow oxygenation during routine treatments. evidence humans generate hyperemic waves after which are consistent with These results challenge long-held assumption primary outcome point new opportunities optimizing precisely modulate outcomes.

Language: Английский

The Cerebrovascular Side of Plasticity: Microvascular Architecture across Health and Neurodegenerative and Vascular Diseases DOI Creative Commons
Marialuisa Zedde, Rosario Pascarella

Brain Sciences, Journal Year: 2024, Volume and Issue: 14(10), P. 983 - 983

Published: Sept. 28, 2024

The delivery of nutrients to the brain is provided by a 600 km network capillaries and microvessels. Indeed, highly energy demanding and, among total amount 100 billion neurons, each neuron located just 10–20 μm from capillary. This vascular also forms part blood–brain barrier (BBB), which maintains brain’s stable environment regulating chemical balance, immune cell transport, blocking toxins. Typically, microvascular endothelial cells (BMECs) have low turnover, indicating cerebrovascular structure. However, this structure can adapt significantly due development, aging, injury, or disease. Temporary neural activity changes are managed expansion contraction arterioles capillaries. Hypoxia leads significant remodeling architecture pathological been documented in aging neurodegenerative conditions. These often involve BMEC proliferation capillary segments, linked with local neuronal cognitive function. Cerebrovascular plasticity, especially arterioles, capillaries, venules, varies over different time scales health, diseases. Rapid cerebral blood flow (CBF) occur within seconds increased activity. Prolonged structure, influenced consistent environmental factors, take weeks. Development bring months years, aging-associated plasticity improved exercise. Injuries cause rapid damage but be repaired weeks months, while diseases slow, varied years. In addition, if animal models may provide useful dynamic vivo information about humans more complex investigate hypothesis glymphatic system together Magnetic Resonance Imaging (MRI) techniques could clues future.

Language: Английский

Citations

6
Low-cost physiology and behavioral monitor for intravital imaging in small mammals DOI Creative Commons
Yuntao Li, Alfredo Cárdenas‐Rivera, Chang Liu

et al.

Neurophotonics, Journal Year: 2025, Volume and Issue: 12(01)

Published: Jan. 25, 2025

SignificanceFunctional brain imaging experiments in awake animals require meticulous monitoring of animal behavior to screen for spontaneous behavioral events. Although these events occur naturally, they can alter cell signaling and hemodynamic activity the confound functional measurements.AimWe developed a centralized, user-friendly, stand-alone platform that includes an fixation frame, compact peripheral sensors, portable data acquisition system. The affordable, integrated benefit by motion detection alertness levels as complementary readouts measurements.ApproachA custom system was designed using powerful, inexpensive microcomputer. We customized accelerometer miniature camera modules efficient, real-time pupil diameter. then tested validated platform's performance with optical intrinsic signal GCaMP fluorescence calcium activation mice.ResultsThe shows promise detecting dilation while imaging. Stimulus-induced found initiate earlier than cortical hemodynamics slower rise time. Compared neuronal response, stimulus-induced initiated later time.ConclusionsWe monitor dynamics. device be easily coupled synchronized systems behavior, alertness, studies.

Language: Английский

Citations

0
Contributions of synaptic glutamate versus neuronal spiking activity to cerebral vascular responses in awake mice DOI
Jenna M Peretin, Christopher G. Cover, Alberto L. Vazquez

et al.

Journal of Cerebral Blood Flow & Metabolism, Journal Year: 2025, Volume and Issue: unknown

Published: May 15, 2025

Neurovascular coupling is the temporal relationship between neuronal activity and regional blood flow changes presumably to meet high metabolic demands of brain. Prior fMRI studies have reported excitatory synaptic transmission as more metabolically demanding than spiking, thus correlating better with cerebral hemodynamics. To investigate this finding newer optical imaging techniques, we used fluorescent markers for extracellular glutamate intracellular calcium directly measure relationships spiking activities on local vascular in awake mice under evoked spontaneous conditions. determine which signal predicts hemodynamic responses, a linear convolution model. Using wide field (WFOI), observed peak fluorescence values 0.38% 5.60% calcium, respectively, whisker stimulation, 3.13% 35.77%, using two-photon microscopy (2PM). Following mean R 2 were 0.64 0.79 WFOI 0.67 0.56, 2PM. From resting-state, 0.73 0.68 respectively. Altogether, both signals perform similarly predicting no significant differences their prediction efficacy.

Language: Английский

Citations

0
Electroconvulsive therapy generates a postictal wave of spreading depolarization in mice and humans DOI Creative Commons
Zachary P. Rosenthal, Joseph B. Majeski, Ala Somarowthu

et al.

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: May 18, 2025

Electroconvulsive therapy (ECT) is a fast-acting, highly effective, and safe treatment for medication-resistant depression. Historically, the clinical benefits of ECT have been attributed to generating controlled seizure; however, underlying neurobiology understudied unresolved. Using optical neuroimaging neural activity hemodynamics in mouse model ECT, we demonstrated that second brain event follows seizure: cortical spreading depolarization (CSD). We found pulse parameters electrode configuration directly shaped wave dynamics seizure subsequent CSD. To translate these findings human patients, used non-invasive diffuse monitoring cerebral blood flow oxygenation during routine treatments. observed brains reliably generate hyperemic waves after which are consistent with These results challenge long-held assumption primary outcome point new opportunities optimizing stimulation outcomes.

Language: Английский

Citations

0
Arousal as a universal embedding for spatiotemporal brain dynamics DOI Creative Commons
Ryan V. Raut, Zachary P. Rosenthal, Xiaodan Wang

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 8, 2023

Neural activity in awake organisms shows widespread and spatiotemporally diverse correlations with behavioral physiological measurements. We propose that this covariation reflects part the dynamics of a unified, multidimensional arousal-related process regulates brain-wide physiology on timescale seconds. By framing interpretation within dynamical systems theory, we arrive at surprising prediction: single, scalar measurement arousal (e.g., pupil diameter) should suffice to reconstruct continuous evolution multidimensional, spatiotemporal measurements large-scale brain physiology. To test hypothesis, perform multimodal, cortex-wide optical imaging monitoring mice. demonstrate neuronal calcium, metabolism, blood-oxygen can be accurately parsimoniously modeled from low-dimensional state-space reconstructed time history diameter. Extending framework electrophysiological Allen Brain Observatory, ability integrate experimental data into unified generative model via mappings an intrinsic manifold. Our results support hypothesis spontaneous, spatially structured fluctuations physiology-widely interpreted reflect regionally-specific neural communication-are large reflections process. This enriched view has broad implications for interpreting observations brain, body, behavior as measured across modalities, contexts, scales.

Language: Английский

Citations

7
Electroconvulsive therapy generates a hidden wave after seizure DOI Creative Commons
Zachary P. Rosenthal, Joseph B. Majeski, Ala Somarowthu

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 1, 2024

Abstract Electroconvulsive therapy (ECT) is a fast-acting, highly effective, and safe treatment for medication-resistant depression. Historically, the clinical benefits of ECT have been attributed to generating controlled seizure; however, underlying neurobiology understudied remains largely unresolved. Using optical neuroimaging probe neural activity hemodynamics in mouse model ECT, we demonstrated that second brain event follows seizure: cortical spreading depolarization (CSD). We further found stimulation pulse parameters electrode configuration directly shaped wave dynamics seizure subsequent CSD. To translate these findings human patients, tested presence hemodynamic signatures post-ictal CSD using non-invasive diffuse monitoring cerebral blood flow oxygenation during routine treatments. evidence humans generate hyperemic waves after which are consistent with These results challenge long-held assumption primary outcome point new opportunities optimizing precisely modulate outcomes.

Language: Английский

Citations

0