Long-term dechlorination performance of the Dehalococcoides-augmented bioelectrochemical system and the functional microorganism identification DOI
Lingyu Meng,

Ryuya Tomita,

Zhiling Li

et al.

Electrochimica Acta, Journal Year: 2024, Volume and Issue: 506, P. 144969 - 144969

Published: Sept. 6, 2024

Language: Английский

Metabolic Synergy of Dehalococcoides Populations Leading to Greater Reductive Dechlorination of Polychlorinated Biphenyls DOI
Chen Chen, Guofang Xu, Matthew J. Rogers

et al.

Environmental Science & Technology, Journal Year: 2024, Volume and Issue: 58(5), P. 2384 - 2392

Published: Jan. 24, 2024

Polychlorinated biphenyls (PCBs) are dioxin-like pollutants that cause persistent harm to life. Organohalide-respiring bacteria (OHRB) can detoxify PCBs via reductive dechlorination, but individual OHRB potent in dechlorinating only specific PCB congeners, restricting the extent of dechlorination. Moreover, low biomass frequently leads slow natural attenuation at contaminated sites. Here we constructed defined microbial consortia comprising various combinations PCB-dechlorinating Dehalococcoides strains (CG1, CG4, and CG5) successfully enhance Specifically, consisting CG1 CG4 removed 0.28–0.44 0.23–0.25 more chlorine per from Aroclor1260 Aroclor1254, respectively, compared strains, which was attributed emergence new dechlorination pathways consortia. Notably, different populations exhibited similar growth when cocultivated, temporal differences expression dehalogenase genes indicated their metabolic synergy. Bioaugmentation with or led greater wetland sediments, augmentation consortium resulted greatest These findings collectively suggest simultaneous application multiple catalyze complementary pathways, is an effective strategy accelerate

Language: Английский

Citations

9
Global prevalence of organohalide-respiring bacteria dechlorinating polychlorinated biphenyls in sewage sludge DOI Creative Commons
Guofang Xu, Siyan Zhao, Matthew J. Rogers

et al.

Microbiome, Journal Year: 2024, Volume and Issue: 12(1)

Published: March 16, 2024

Abstract Background Massive amounts of sewage sludge are generated during biological treatment and commonly subjected to anaerobic digestion, land application, landfill disposal. Concurrently, persistent organic pollutants (POPs) frequently found in disposal systems, posing significant risks both human health wildlife. Metabolically versatile microorganisms originating from inevitably introduced potentially affecting the fate POPs. However, there is currently a dearth comprehensive assessments regarding capability microbiota geographically disparate regions attenuate POPs underpinning microbiomes. Results Here we report global prevalence organohalide-respiring bacteria (OHRB) known for their capacity sludge, with an occurrence frequency ~50% investigated samples (605 1186). Subsequent laboratory tests revealed microbial reductive dechlorination polychlorinated biphenyls (PCBs), one most notorious categories POPs, 80 out 84 microcosms via various pathways. Most chlorines were removed para - meta -positions PCBs; nevertheless, ortho -dechlorination PCBs also occurred widely, although lower extents. Abundances several well-characterized OHRB genera ( Dehalococcoides , Dehalogenimonas Dehalobacter ) uncultivated Dehalococcoidia lineages increased incubation positively correlated PCB dechlorination, suggesting involvement dechlorinating PCBs. The previously identified dehalogenase (RDase) genes pcbA4 pcbA5 tended coexist microcosms, but low ratios these RDase abundance indicated existence undescribed RDases sludge. Microbial community analyses positive correlation between biodiversity activity was apparent threshold co-occurrence network complexity beyond which decreased. Conclusions Our findings that exhibited nearly ubiquitous indicate widespread nonnegligible impacts on systems. diverse suggests as alternative source obtain POP-attenuating consortia calls further exploration populations

Language: Английский

Citations

8
An innovative permeable reactive bio-barrier to remediate trichloroethene-contaminated groundwater: A field study DOI
Che‐Wei Lu, Kai‐Hung Lo,

Sun-Chong Wang

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 920, P. 170885 - 170885

Published: Feb. 9, 2024

Language: Английский

Citations

6
Efficient biostimulation of microbial dechlorination of polychlorinated biphenyls by acetate and lactate under nitrate reducing conditions: Insights into dechlorination pathways and functional genes DOI
Yan Xu, Ying Wang,

Zheng An

et al.

Journal of Hazardous Materials, Journal Year: 2024, Volume and Issue: 468, P. 133775 - 133775

Published: Feb. 11, 2024

Language: Английский

Citations

5
Sulfate-Driven Microbial Collaboration for Synergistic Remediation of Chloroethene-Heavy Metal Pollution DOI
Zhengtao Li, He‐Ping Zhao

Water Research, Journal Year: 2024, Volume and Issue: 268, P. 122738 - 122738

Published: Nov. 2, 2024

Language: Английский

Citations

4
CO-driven electron and carbon flux fuels synergistic microbial reductive dechlorination DOI Creative Commons
Jingjing Wang, Xiuying Li, Huijuan Jin

et al.

Microbiome, Journal Year: 2024, Volume and Issue: 12(1)

Published: Aug. 20, 2024

Carbon monoxide (CO), hypothetically linked to prebiotic biosynthesis and possibly the origin of life, emerges as a substantive growth substrate for numerous microorganisms. In anoxic environments, coupling CO oxidation with hydrogen (H

Language: Английский

Citations

3
Insights into anaerobic biotransformation of Polychlorinated Biphenyls in Dehalococcoides mccartyi CG1 through Kinetic and Stable Isotopic Analysis DOI
Yanting Zhang, Yan‐Hong Zeng, Chenchen Huang

et al.

Environmental Pollution, Journal Year: 2025, Volume and Issue: unknown, P. 125826 - 125826

Published: Feb. 1, 2025

Language: Английский

Citations

0
Enhanced dechlorination and degradation of Aroclor 1260 by resuscitation-promoting factor under alternating anaerobic-aerobic conditions: Superior performance and associated microbial populations DOI
Songlin Fei, Xiaonan Li,

Zhen Han

et al.

Environmental Research, Journal Year: 2025, Volume and Issue: unknown, P. 121531 - 121531

Published: April 1, 2025

Language: Английский

Citations

0
Chemical-physical parameters and microbial community changes induced by electrodes polarization inhibit PCB dechlorination in a marine sediment DOI Creative Commons
Alberto Botti, Eliana Musmeci,

Bruna Matturro

et al.

Journal of Hazardous Materials, Journal Year: 2024, Volume and Issue: 469, P. 133878 - 133878

Published: Feb. 24, 2024

Microbial reductive dechlorination of organohalogenated pollutants is often limited by the scarcity electron donors, that can be overcome with microbial electrochemical technologies (METs). In this study, polarized electrodes buried in marine sediment microcosms were investigated to stimulate PCB under potentiostatic (-0.7 V vs Ag/AgCl) and galvanostatic conditions (0.025 mA·cm-2-0.05 mA·cm-2), using graphite rod as cathode iron plate sacrificial anode. A single circuit a novel two antiparallel circuits configuration (2AP) investigated. Single polarization impacted pH redox potential (ORP) proportionally intensity electrical input inhibited dechlorination. The effects on sediment's ORP, along inhibition dechlorination, mitigated 2AP system. Electrodes stimulated sulfate-reduction promoted enrichment bacterial clades potentially involved well sulfur oxidation. This suggested electrons provided consumed competitors organohalide respiring bacteria specifically sequestered cycling, which may represent main factor limiting applicability METs for stimulating sediments. Polychlorinated biphenyls (PCBs) are known persistent organic still lacking an effective remediation strategy. Electron donors play crucial role priming lowers PCBs' toxicity. Electrochemical Technologies (METs) have been proposed dehalogenation chlorinated solvents freshwater environments. work, applied respiration. Our results show effectiveness processes environments compromised cycling sequestering electrons, suggests directions context-specific challenges.

Language: Английский

Citations

1
Long-term dechlorination performance of the Dehalococcoides-augmented bioelectrochemical system and the functional microorganism identification DOI
Lingyu Meng,

Ryuya Tomita,

Zhiling Li

et al.

Electrochimica Acta, Journal Year: 2024, Volume and Issue: 506, P. 144969 - 144969

Published: Sept. 6, 2024

Language: Английский

Citations

0