Underexplored Organohalide-Respiring Bacteria in Sewage Sludge Debrominating Polybrominated Diphenyl Ethers

Environmental Science & Technology, Journal Year: 2024, Volume and Issue: unknown

Published: July 20, 2024

Polybrominated diphenyl ethers (PBDEs) are persistent organic pollutants prevalent in the environment. Organohalide-respiring bacteria (OHRB) can attenuate PBDEs via reductive debromination, but often producing toxic end-products. Debromination of to ether remains a rare phenomenon and is so far specifically associated with Dehalococcoides isolated from e-waste polluted sites. The occurrence PBDE debromination other ecosystems underpinning OHRB underexplored. Here we found that common trait sewage sludge microbiota, was produced as end-product at varying quantities (0.6-52.9% mol parent PBDEs) 76 84 cultures established bioreactor sludge. Diverse pathways converting ether, including several new routes, were identified. Although contributed Dehalogenimonas, Dehalobacter, uncultivated Dehalococcoidia likely played more important roles than previously recognized. Multiple dehalogenase genes (including bdeA, pcbA4, pteA, tceA) also coexisted Collectively, these findings contribute enhancing our comprehension environmental fate PBDEs, expanding diversity microorganisms catalyzing developing consortia for bioremediation application.

Language: Английский

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Insights into anaerobic biotransformation of Polychlorinated Biphenyls in Dehalococcoides mccartyi CG1 through Kinetic and Stable Isotopic Analysis

Environmental Pollution, Journal Year: 2025, Volume and Issue: unknown, P. 125826 - 125826

Published: Feb. 1, 2025

Language: Английский

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Microbial and chemically induced reductive dechlorination of polychlorinated biphenyls in the environment

Environmental Science and Pollution Research, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 7, 2025

Language: Английский

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Enhanced dechlorination and degradation of Aroclor 1260 by resuscitation-promoting factor under alternating anaerobic-aerobic conditions: Superior performance and associated microbial populations

Environmental Research, Journal Year: 2025, Volume and Issue: unknown, P. 121531 - 121531

Published: April 1, 2025

Language: Английский

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High-Throughput Screening of Microbial Reductive Dechlorination of Polychlorinated Biphenyls: Patterns in Reactivity and Pathways

Environmental Science & Technology, Journal Year: 2025, Volume and Issue: unknown

Published: April 7, 2025

Polychlorinated biphenyls (PCBs) are pervasive pollutants that pose risks to ecosystems and human health. Microbial reductive dehalogenation plays crucial roles in attenuating PCBs, but comprehensive insights into PCB dechlorination pathways, reactivity, governing factors limited by the vast number of congeners costly experimental approaches. We address this challenge establishing a high-throughput vitro assay approach (HINVARD), which increases test throughput 30-fold enhances reagents cell utilization efficiency over 10-fold compared conventional methods. Using HINVARD, we screened 61 across 9 enrichment cultures 3 Dehalococcoides isolates, identifying active 31-44 congeners. Results showed congener properties (chlorine substitution patterns, steric hindrance, solubility) primarily determine potential, leading consistent reactivity trends cultures. In contrast, different organohalide-respiring bacteria catalyzed distinct preferentially removing para- or meta-chlorines. Structural modeling dehalogenases revealed unique binding orientations substrate specificity, offering molecular these pathways. This study provides high-efficiency strategy for investigating microbial dehalogenation, yielding first understanding patterns mechanisms. These findings guide design tailored consortia effective bioremediation.

Language: Английский

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Combatting multiple aromatic organohalide pollutants in sediments by bioaugmentation with a single Dehalococcoides

Water Research, Journal Year: 2024, Volume and Issue: 255, P. 121447 - 121447

Published: March 11, 2024

Language: Английский

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Dual C and Cl compound-specific isotope analysis and metagenomic insights into the degradation of the pesticide methoxychlor

Journal of Hazardous Materials, Journal Year: 2024, Volume and Issue: 480, P. 135929 - 135929

Published: Sept. 20, 2024

Language: Английский

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Understanding the sources, function, and irreplaceable role of cobamides in organohalide-respiring bacteria

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: July 30, 2024

Halogenated organic compounds are persistent pollutants that pose a serious threat to human health and the safety of ecosystems. Cobamides essential cofactors for reductive dehalogenases (RDase) in organohalide-respiring bacteria (OHRB), which catalyze dehalogenation process. This review systematically summarizes impact cobamides on organohalide respiration. The catalytic processes cobamide also discussed. Additionally, we examine OHRB, cannot synthesize must obtain it from environment through salvage pathway; co-culture with producer is more beneficial possible. aims help readers better understand importance function dehalogenation. presented information can aid development bioremediation strategies.

Language: Английский

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Remediation of PBDE-Contaminated soil using biochar-based bacterial consortium QY2Y

Journal of Cleaner Production, Journal Year: 2024, Volume and Issue: unknown, P. 143982 - 143982

Published: Oct. 1, 2024

Language: Английский

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