Virus-like structures for combination antigen protein mRNA vaccination DOI Creative Commons
Jingjing Zhang, Yanmei Li,

Fengyuan Zeng

et al.

Nature Nanotechnology, Journal Year: 2024, Volume and Issue: 19(8), P. 1224 - 1233

Published: May 27, 2024

Abstract Improved vaccination requires better delivery of antigens and activation the natural immune response. Here we report a lipid nanoparticle system with capacity to carry antigens, including mRNA proteins, which is formed into virus-like structure by surface decoration spike demonstrating application against SARS-CoV-2 variants. The strategy uses S1 protein from Omicron BA.1 on deliver XBB.1. particle enables specific augmentation mRNAs expressed in human respiratory epithelial cells macrophages via interaction ACE2 or DC-SIGN receptors. Activation dendritic demonstrated same receptor binding. combination increases antibody response BALB/c mice compared vaccines alone. Our exploration mechanism this robust immunity suggests it might involve cross-presentation diverse subsets ranging activated innate signals adaptive signals.

Language: Английский

Intranasal influenza-vectored COVID-19 vaccine restrains the SARS-CoV-2 inflammatory response in hamsters DOI Creative Commons
Liang Zhang, Yao Jiang,

Jinhang He

et al.

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: July 11, 2023

The emergence of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) variants and "anatomical escape" characteristics threaten the effectiveness current coronavirus disease 2019 (COVID-19) vaccines. There is an urgent need to understand immunological mechanism broad-spectrum tract protection guide broader vaccines development. Here we investigate immune responses induced by NS1-deleted influenza virus vectored intranasal COVID-19 vaccine (dNS1-RBD) which provides against SARS-CoV-2 in hamsters. Intranasal delivery dNS1-RBD induces innate immunity, trained immunity tissue-resident memory T cells covering upper lower tract. It restrains inflammatory response suppressing early phase viral load post challenge attenuating pro-inflammatory cytokine (Il6, Il1b, Ifng) levels, thereby reducing excess immune-induced tissue injury compared with control group. By inducing local cellular represents a strategy reduce burden.

Language: Английский

Citations

33
Next Generation Mucosal Vaccine Strategy for Respiratory Pathogens DOI Creative Commons
Farokh Dotiwala, Arun Kumar Upadhyay

Vaccines, Journal Year: 2023, Volume and Issue: 11(10), P. 1585 - 1585

Published: Oct. 12, 2023

Inducing humoral and cytotoxic mucosal immunity at the sites of pathogen entry has potential to prevent infection from getting established. This is different systemic vaccination, which protects against development symptoms. The field vaccination seen fewer technological advances compared nucleic acid subunit vaccine for injectable platforms. advent next-generation adenoviral vectors given a boost research. Basic research into mechanisms regulating innate adaptive discovery effective safe adjuvants will continue improve design. results clinical trials inhaled COVID-19 vaccines demonstrate their ability induce proliferation T cells production secreted IgA IgG antibodies locally, unlike intramuscular vaccinations. However, these immune responses par with review summarizes function respiratory mucosa-associated lymphoid tissue advantages that provide as

Language: Английский

Citations

29
COVID-19 annual update: a narrative review DOI Creative Commons
Michela Biancolella, Vito Luigi Colona, Lucio Luzzatto

et al.

Human Genomics, Journal Year: 2023, Volume and Issue: 17(1)

Published: July 24, 2023

Abstract Three and a half years after the pandemic outbreak, now that WHO has formally declared emergency is over, COVID-19 still significant global issue. Here, we focus on recent developments in genetic genomic research COVID-19, give an outlook state-of-the-art therapeutical approaches, as gradually transitioning to endemic situation. The sequencing characterization of rare alleles different populations made it possible identify numerous genes affect either susceptibility or severity disease. These findings provide beginning new avenues pan-ethnic therapeutic well potential screening protocols. causative virus, SARS-CoV-2, spotlight, but novel threatening virus could appear anywhere at any time. Therefore, continued vigilance further warranted. We also note emphatically prevent future pandemics other world-wide health crises, imperative capitalize what have learnt from COVID-19: specifically, regarding its origins, world’s response, insufficient preparedness. This requires unprecedented international collaboration timely data sharing for coordination effective response rapid implementation containment measures.

Language: Английский

Citations

28
Infectious virus shedding duration reflects secretory IgA antibody response latency after SARS-CoV-2 infection DOI Creative Commons
Shô Miyamoto, Takara Nishiyama, Akira Ueno

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2023, Volume and Issue: 120(52)

Published: Dec. 22, 2023

Infectious virus shedding from individuals infected with severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) is used to estimate human-to-human transmission risk. Control of SARS-CoV-2 requires identifying the immune correlates that protect infectious shedding. Mucosal immunity prevents infection by SARS-CoV-2, which replicates in epithelium and spreads rapidly other hosts. However, whether mucosal SARS-CoV-2-infected unknown. We examined relationship between viral RNA dynamics, duration shedding, antibody responses during infection. Anti-spike secretory IgA antibodies (S-IgA) reduced load infectivity more than anti-spike IgG/IgA nasopharyngeal samples. Compared response, S-IgA post-infection affected dynamics predicted regardless history. These findings highlight importance for preventing transmission. Developing medical countermeasures shorten response time may help control prevent future pandemics.

Language: Английский

Citations

24
SARS-CoV-2 correlates of protection from infection against variants of concern DOI
Kaiyuan Sun, Jinal N. Bhiman, Stefano Tempia

et al.

Nature Medicine, Journal Year: 2024, Volume and Issue: 30(10), P. 2805 - 2812

Published: July 26, 2024

Language: Английский

Citations

11
Mucosal correlates of protection after influenza viral challenge of vaccinated and unvaccinated healthy volunteers DOI Creative Commons
Rachel Bean, Luca T. Giurgea, Alison Han

et al.

mBio, Journal Year: 2024, Volume and Issue: 15(2)

Published: Jan. 9, 2024

The devastating potential of influenza has been well known for over 100 years. Despite the development vaccines since middle 20th century, continues to be responsible substantial global morbidity and mortality. To develop next-generation with enhanced effectiveness, we must synthesize our understanding complex immune mechanisms culminating in protection. Our study outlines differences responses vaccine infection, identifying gaps vaccine-induced immunity, particularly at level nasal mucosa. Furthermore, this research underscores need refine imperfect models while recognizing pitfalls past future attempts identify measure correlates

Language: Английский

Citations

9
SARS-CoV-2 resistance to monoclonal antibodies and small-molecule drugs DOI Creative Commons
Sho Iketani, David D. Ho

Cell chemical biology, Journal Year: 2024, Volume and Issue: 31(4), P. 632 - 657

Published: April 1, 2024

Over four years have passed since the beginning of COVID-19 pandemic. The scientific response has been rapid and effective, with many therapeutic monoclonal antibodies small molecules developed for clinical use. However, given ability viruses to become resistant antivirals, it is perhaps no surprise that field identified resistance nearly all these compounds. Here, we provide a comprehensive review profile each therapeutics. We hope this resource provides an atlas mutations be aware agent, particularly as springboard considerations next generation antivirals. Finally, discuss outlook thoughts moving forward in how continue manage this, next,

Language: Английский

Citations

9
Primary nasal influenza infection rewires tissue-scale memory response dynamics DOI
Samuel W. Kazer,

Colette Matysiak Match,

Erica M. Langan

et al.

Immunity, Journal Year: 2024, Volume and Issue: 57(8), P. 1955 - 1974.e8

Published: July 3, 2024

Language: Английский

Citations

8
Impact of RSVpreF vaccination on reducing the burden of respiratory syncytial virus in infants and older adults DOI Creative Commons
Zhanwei Du,

Abhishek Pandey,

Seyed M. Moghadas

et al.

Nature Medicine, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 9, 2025

Language: Английский

Citations

1
A research and development (R&D) roadmap for broadly protective coronavirus vaccines: A pandemic preparedness strategy DOI Creative Commons
Penny L. Moore,

T. Leighton,

Julie Ostrowsky

et al.

Vaccine, Journal Year: 2023, Volume and Issue: 41(13), P. 2101 - 2112

Published: Feb. 21, 2023

Broadly protective coronavirus vaccines are an important tool for protecting against future SARS-CoV-2 variants and could play a critical role in mitigating the impact of outbreaks or pandemics caused by novel coronaviruses. The Coronavirus Vaccines Research Development (R&D) Roadmap (CVR) is aimed at promoting development such vaccines. CVR, funded Bill & Melinda Gates Foundation Rockefeller Foundation, was generated through collaborative iterative process, which led Center Infectious Disease Policy (CIDRAP) University Minnesota involved 50 international subject matter experts recognized leaders field. This report summarizes major issues areas research outlined CVR identifies high-priority milestones. covers 6-year timeframe organized into five topic areas: virology, immunology, vaccinology, animal human infection models, policy finance. Included each area key barriers, gaps, strategic goals, milestones, additional R&D priorities. roadmap includes 20 goals 86 26 ranked as high priority. By identifying issues, milestones addressing them, provides framework to guide funding campaigns that promote broadly

Language: Английский

Citations

20