Lung Microbiota: From Healthy Lungs to Development of Chronic Obstructive Pulmonary Disease

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(4), P. 1403 - 1403

Published: Feb. 7, 2025

Lung health is dependent on a complex picture of the lung microbiota composed bacteriobiota, mycobiota, and virome. The studies have demonstrated that has crucial role in host protection by regulating innate adaptive immunity. Chronic obstructive pulmonary disease (COPD) an inflammatory featuring changed composition diversity, known as dysbiosis. dysbiosis increases with progress COPD during exacerbation. Two models been proposed: inflammation cycles disturbance bacterial interactome. Still, it unknown if driving factor pathogenesis belongs to or microbiota. Recently, host–microbiota microbe–microbe interactions highlighted COPD, but mechanisms behind these need further exploration. function gut–lung axis for maintenance affected COPD. application probiotics resulted host–beneficial effects, likely future this field will aid therapy In review, microbiota, molecular mechanisms, clinical aspects relating are comprehensively provided.

Language: Английский

---

Identification and experimental validation of PYCARD as a crucial PANoptosis-related gene for immune response and inflammation in COPD

APOPTOSIS, Journal Year: 2024, Volume and Issue: 29(11-12), P. 2091 - 2107

Published: April 23, 2024

Language: Английский

---

Lower Airway Dysbiosis Augments Lung Inflammatory Injury in Mild-to-Moderate Chronic Obstructive Pulmonary Disease

American Journal of Respiratory and Critical Care Medicine, Journal Year: 2023, Volume and Issue: 208(10), P. 1101 - 1114

Published: Sept. 7, 2023

Chronic obstructive pulmonary disease (COPD) is associated with high morbidity, mortality, and healthcare costs. Cigarette smoke a causative factor; however, not all heavy smokers develop COPD. Microbial colonization infections are contributing factors to progression in advanced stages.

Language: Английский

---

The future drug treatment of COPD

European Respiratory Society eBooks, Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 26, 2024

Language: Английский

---

The Lung Microbiome

The Journal of Immunology, Journal Year: 2024, Volume and Issue: 212(8), P. 1269 - 1275

Published: April 1, 2024

Abstract Although the lungs were once considered a sterile environment, advances in sequencing technology have revealed dynamic, low-biomass communities respiratory tract, even health. Key features of these communities—composition, diversity, and burden—are consistently altered lung disease, associate with host physiology immunity, can predict clinical outcomes. initial studies microbiome descriptive, recent leveraged to identify metabolically active microbes potential associations their immunomodulatory by-products disease. In this brief review, we discuss novel insights airway disease parenchymal exploring host–microbiome interactions pathogenesis. We also complex between gut oropharyngeal microbiota immunobiology. Our advancing knowledge will provide targets acute chronic may facilitate development new therapeutic strategies.

Language: Английский

---

Human genetic associations of the airway microbiome in chronic obstructive pulmonary disease

Respiratory Research, Journal Year: 2024, Volume and Issue: 25(1)

Published: April 16, 2024

Little is known about the relationships between human genetics and airway microbiome. Deeply sequenced metagenomics, by simultaneously characterizing microbiome host genetics, provide a unique opportunity to assess microbiome-host genetic associations. Here we performed co-profiling of with identification over 5 million single nucleotide polymorphisms (SNPs) through deep metagenomic sequencing in sputum 99 chronic obstructive pulmonary disease (COPD) 36 healthy individuals. Host variation was most significant factor associated except for geography status, its top principal components accounting 12.11% variability. Within COPD individuals, 113 SNPs mapped candidate genes reported as genetically exhibited associations 29 microbial species 48 functional modules (P < 1 × 10

Language: Английский

---

Pathobiological signatures of dysbiotic lung injury in pediatric patients undergoing stem cell transplantation

Nature Medicine, Journal Year: 2024, Volume and Issue: 30(7), P. 1982 - 1993

Published: May 23, 2024

Abstract Hematopoietic cell transplantation (HCT) uses cytotoxic chemotherapy and/or radiation followed by intravenous infusion of stem cells to cure malignancies, bone marrow failure and inborn errors immunity, hemoglobin metabolism. Lung injury is a known complication the process, due in part disruption pulmonary microenvironment insults such as infection, alloreactive inflammation cellular toxicity. How microorganisms, immunity respiratory epithelium interact contribute lung uncertain, limiting development prevention treatment strategies. Here we used 278 bronchoalveolar lavage (BAL) fluid samples study 229 pediatric patients who have undergone HCT treated at 32 children’s hospitals between 2014 2022. By leveraging paired microbiome human gene expression data, identified high-risk BAL compositions associated with in-hospital mortality ( P = 0.007). Disadvantageous profiles included bacterial overgrowth neutrophilic inflammation, contraction epithelial fibroproliferation profound commensal depletion viral staphylococcal enrichment, lymphocytic activation injury, were replicated an independent cohort from Netherlands 0.022). In addition, broad array previously occult pathogens was identified, well strong link antibiotic exposure, enrichment viruses fungi. Together these lung–immune system–microorganism interactions clarify important drivers fatal HCT. Further investigation needed determine how personalized interpretation heterogeneous microenvironments may be improve outcomes.

Language: Английский

---

Role of nasal microbiota in regulating host anti-influenza immunity in dogs

Microbiome, Journal Year: 2025, Volume and Issue: 13(1)

Published: Jan. 27, 2025

Numerous studies have confirmed a close relationship between the pathogenicity of influenza and respiratory microbiota, but mechanistic basis for this is poorly defined. Also, majority these been conducted on murine models, it remains unclear how far findings can be extrapolated from models to other animals. Considering that A virus increasingly recognized as an important canine pathogen, study investigated cross-talk nasal lung tissues mediated by microbes its association with susceptibility in beagle dog model. Using 16S rRNA gene sequencing, combined comparative transcriptomic, anatomical, histological examinations, we viral presence, expression profiles, microbiota cavity after infection beagles antibiotic-induced dysbiosis. Our data showed dysbiosis microbiome exacerbates influenza-induced disease epithelial barrier disruption, impairs host antiviral responses lung. Moreover, dysregulation disturbance microbiota. Further, also identified strain Lactobacillus plantarum isolated significant effect vitro, found activity might associated activation interferon (IFN) pathway modulation impaired autophagy flux induced infection. investigation reveals exerts prominent impact responses, inflammation thresholds, mucosal integrity during Lactobacilli, part may contribute defenses modulating IFN pathways. Collectively, underscores importance homeostasis maintaining health.

Language: Английский

---

S1PR1-biased activation drives the resolution of endothelial dysfunction-associated inflammatory diseases by maintaining endothelial integrity

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: Feb. 20, 2025

G protein-coupled sphingosine-1-phosphate receptor 1 (S1PR1), a drug target for inflammatory bowel disease (IBD), enables immune cells to egress from lymph nodes, but the treatment increases risk of immunosuppression. The functional signaling pathway triggered by S1PR1 activation in endothelial and its therapeutic application remains unclear. Here, we showed that is highly expressed IBD patients positively correlated with markers. Gi-biased agonist-SAR247799 activated reversed pathology male mouse organoid models protecting integrity barrier without affecting cell egress. Cryo-electron microscopy structure S1PR1-Gi complex bound SAR247799 resolution 3.47 Å revealed recognition mode biased ligand. With efficacy treating other dysfunction-associated diseases, our study offers mechanistic insights into agonist represents strategy treatment.

Language: Английский

---

Effect of Multidimensional Integrated Lung Protection Measures in Elderly Patients With Fragile Lungs or Combined Lung Dysfunction by Regulating AMPK/SIRT1 Pathway

Journal of Cellular and Molecular Medicine, Journal Year: 2025, Volume and Issue: 29(4)

Published: Feb. 1, 2025

ABSTRACT Fragile lungs or lung dysfunction can significantly impact a patient's quality of life. Currently, no specific treatment exists to prevent in elderly patients. The detailed mechanism fragile patients remains elusive, and this study aimed clarify it. General data blood specimens were obtained from with dysfunction. mice exposed cigarette smoke using smoking apparatus induce model. Blood samples tissues collected all groups for further testing. haematoxylin–eosin (HE) staining, immunofluorescence, Western blot, flow cytometry quantitative reverse transcriptase PCR (qRT‐PCR) used elucidate the molecular mechanisms multidimensional integrated protection measures (MILPM) by targeting AMP‐activated protein kinase (AMPK)/Sirtuin 1 (SIRT1) pathway. results indicated that upregulation AMPK/SIRT1 signalling pathway accelerates process, whereas downregulation Similarly, change forced vital capacity (FVC), total (TLC) levels is associated reducing their serve as preventative method against development. Upregulation accelerate process

Language: Английский

---