Microorganisms,
Journal Year:
2024,
Volume and Issue:
12(3), P. 531 - 531
Published: March 6, 2024
Atopic
dermatitis
(AD)
is
a
chronic
and
relapsing
inflammatory
cutaneous
disease.
The
role
of
host
defense
microbial
virulence
factors
in
Staphylococcus
aureus
skin
colonization,
infection,
inflammation
perpetuation
AD
remains
an
area
current
research
focus.
Extracellular
vesicles
(EV)
mediate
cell-to-cell
communication
by
transporting
delivering
bioactive
molecules,
such
as
nucleic
acids,
proteins,
enzymes,
to
recipient
cells.
spontaneously
secretes
extracellular
(SA-derived
EVs),
which
spread
throughout
the
layers.
Previous
has
shown
that
SA-derived
EVs
from
patients
can
trigger
cytokine
secretion
keratinocytes,
shape
recruitment
neutrophils
monocytes,
induce
AD-type
lesions
mouse
models,
addition
their
exogenous
worsening
for
In
this
review
article,
we
aim
examine
physiopathology
its
progression,
highlighting
recent
field
exploring
potential
crosstalk
between
microbiota.
Science Advances,
Journal Year:
2025,
Volume and Issue:
11(9)
Published: Feb. 26, 2025
Naturally
occurring
extracellular
vesicles
(EVs)
and
synthetic
nanoparticles
like
liposomes
have
revolutionized
precision
diagnostics
medicine.
EVs
excel
in
biocompatibility
cell
targeting,
while
offer
enhanced
drug
loading
capacity
scalability.
The
clinical
translation
of
is
hindered
by
challenges
including
low
yield
heterogeneity,
whereas
face
rapid
immune
clearance
limited
targeting
efficiency.
To
bridge
these
gaps,
biomimetic
(SVs)
emerged
as
innovative
platforms,
combining
the
advantageous
properties
liposomes.
This
review
emphasizes
critical
aspects
EV
biology,
such
mechanisms
EV-cell
interaction
source-dependent
functionalities
modulation,
tissue
regeneration,
informing
SV
engineering.
We
reviewed
a
broad
array
SVs,
with
focus
on
lipid
bilayered
functionalized
proteins.
These
include
cell-derived
nanovesicles,
protein-functionalized
liposomes,
hybrid
vesicles.
By
addressing
current
highlighting
opportunities,
this
aims
to
advance
SVs
for
transformative
biomedical
applications.
The Journal of Cell Biology,
Journal Year:
2024,
Volume and Issue:
223(6)
Published: March 29, 2024
Phytopathogens
cause
plant
diseases
that
threaten
food
security.
Unlike
mammals,
plants
lack
an
adaptive
immune
system
and
rely
on
their
innate
to
recognize
respond
pathogens.
Plant
response
a
pathogen
attack
requires
precise
coordination
of
intracellular
traffic
signaling.
Spatial
and/or
temporal
defects
in
coordinating
signals
cargo
can
lead
detrimental
effects
cell
development.
The
role
comes
into
critical
focus
when
the
sustains
biotic
stress.
In
this
review,
we
discuss
current
understanding
post-immune
activation
logistics
defense.
Specifically,
packaging
shipping
defense-related
cargo,
rerouting
traffic,
players
enabling
pathogen-mediated
subversion
these
pathways.
We
highlight
roles
cytoskeleton,
cytoskeleton–organelle
bridging
proteins,
secretory
vesicles
maintaining
pathways
exocytic
defense,
acting
as
sentinels
during
attack,
necessary
elements
for
building
wall
barrier
also
identify
points
convergence
between
mammalian
trafficking
defense
unique
responses
illustrate
evolutionary
adaptations
have
undergone
resist
Journal of Nanobiotechnology,
Journal Year:
2024,
Volume and Issue:
22(1)
Published: May 19, 2024
Abstract
Symbiotic
microbial
communities
are
crucial
for
human
health,
and
dysbiosis
is
associated
with
various
diseases.
Plant-derived
nanovesicles
(PDNVs)
have
a
lipid
bilayer
structure
contain
lipids,
metabolites,
proteins,
RNA.
They
offer
unique
advantages
in
regulating
community
homeostasis
treating
diseases
related
to
compared
traditional
drugs.
On
the
one
hand,
lipids
on
PDNVs
serve
as
primary
substances
that
mediate
specific
recognition
uptake
by
bacteria.
other
due
multifactorial
nature
of
PDNVs,
they
potential
enhance
growth
survival
beneficial
bacterial
while
simultaneously
reducing
pathogenicity
harmful
In
addition,
capacity
modulate
metabolism,
thus
facilitating
establishment
harmonious
equilibrium
promoting
stability
within
microbiota.
These
remarkable
attributes
make
promising
therapeutic
approach
conditions,
including
periodontitis,
inflammatory
bowel
disease,
skin
infection
However,
challenges
such
consistency,
isolation
methods,
storage
need
be
addressed
before
clinical
application.
This
review
aims
explore
value
provide
recommendations
their
use
novel
agents
health
protection.
Graphical
mLife,
Journal Year:
2025,
Volume and Issue:
4(1), P. 45 - 54
Published: Feb. 1, 2025
Abstract
RNAi
technologies
have
been
exploited
to
control
viruses,
pests,
oomycetes,
and
fungal
phytopathogens
that
cause
disasters
in
host
plants,
including
many
agronomically
significant
crops.
Double‐stranded
RNA
(dsRNA)
or
small
interfering
(siRNA)
has
applied
as
a
trigger
for
trans‐kingdom
between
hosts
fungi.
However,
it
is
unclear
what
process
mediates
uptake
by
In
this
study,
using
live‐cell
imaging,
we
determined
exogenously
synthesized
(sRNA)
was
indiscriminately
absorbed
into
Verticillium
dahliae
,
notorious
pathogenic
fungus.
Moreover,
the
application
of
endocytic
inhibitors
deletion
endocytic‐related
genes
reduced
efficiency,
showing
absorption
cells
occurs
mainly
through
endocytosis.
addition,
found
endocytosed
fluorescence‐labeled
RNAs
were
partly
colocalized
with
endosome
marker
genes.
Overall,
our
research
concluded
exogenous
could
be
assimilated
V.
pathway.
Unraveling
cytological
mechanism
underlying
holds
importance,
especially
considering
fact
RNAi‐based
strategies
targeting
fungi
are
increasingly
prevalent
realm
crop
protection.
Deleted Journal,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 20, 2025
Abstract
Plant
extracellular
vesicles
(PEVs)
are
nanoscale
secreted
by
plant
cells
with
intact
membrane
architecture,
which
encapsulate
a
diverse
array
of
biomolecules,
including
lipids,
proteins
and
RNA.
They
integral
to
both
intra‐cellular
communication
within
plants
inter‐species
signaling.
Recently,
some
PEVs
have
been
regarded
as
competitive
candidates
for
disease
therapy
due
their
beneficial
components
distinctive
hollow
biomembrane
structure.
However,
the
broader
applications
currently
impeded
several
challenges
complicated
extraction
processes,
compositional
heterogeneity,
lack
reliable
biomarkers
unclear
mechanisms.
A
detailed
comprehension
preparation
techniques
biological
functions
is
essential
leveraging
potential
in
clinical
medicine.
This
article
first
presented
synthesis
current
methodologies
PEV
isolation,
purification
characterization.
Then,
it
revealed
therapeutic
implications
medicines
common
diseases
based
on
bioactive
molecules
inside,
such
cancer,
inflammation,
metabolic
disorders.
We
especially
explored
emerging
role
low
immunogenicity
power
barriers
crossing
drug
delivery
systems,
underscoring
further
industry
applications.
At
last,
bottleneck
problems
vision
were
also
evoke
more
insightful
deliberation.
review
aims
provide
directions
development
PEV‐derived
innovative
drugs.
Journal of Extracellular Vesicles,
Journal Year:
2024,
Volume and Issue:
13(10)
Published: Oct. 1, 2024
Abstract
The
morphogenesis
of
higher
plants
requires
communication
among
distant
organs
throughout
vascular
tissues
(xylem
and
phloem).
Numerous
investigations
have
demonstrated
that
phloem
also
act
as
a
distribution
route
for
signalling
molecules
being
observed
different
macromolecules
translocated
by
the
sap,
including
nucleic
acids
proteins,
change
under
stress
situations.
participation
extracellular
vesicles
(EVs)
in
this
has
been
suggested,
although
little
is
known
about
their
role.
In
fact,
last
decade,
presence
EVs
originated
great
controversy,
where
major
concerns
arose
from
origin,
isolation
methods,
even
appropriate
nomenclature
plant
nanovesicles.
Phloem
sap
exudates
melon
plants,
either
aphid‐free
or
infested
with
Aphis
gossypii
,
were
collected
stem
incision.
After
concentration
(Amicon),
(PhlEVs)
isolated
size
exclusion
chromatography.
PhlEVs
characterised
using
Nanoparticle
Tracking
Analysis,
Transmission
electron
microscopy
proteomic
analysis.
Here
we
confirm
vivo
detection
changes
particles/protein
ratio
composition
response
to
insect
feeding,
revealing
typical
defence
proteins
cargo
well
components
proteasome
complex.
showed
lower
almost
two
times
more
proteolytic
activity
than
plants.
both
cases,
such
was
inhibited
dose‐dependent
manner
inhibitor
MG132.
Our
results
suggest
may
use
mechanism
prepare
themselves
receive
infectious
agents
open
up
possibility
an
evolutionary
conserved
against
pathogens/stresses
eukaryotic
organisms.
