Comparison of the efficacy of alginate nanoparticles containing Cymbopogon citratus essential oil and citral on melanoma and breast cancer cell lines under normoxic and hypoxic conditions DOI Creative Commons

Farnaz Karami,

Mahmoud Osanloo, Hiva Alipanah

et al.

BMC Complementary Medicine and Therapies, Journal Year: 2024, Volume and Issue: 24(1)

Published: Oct. 19, 2024

Solid tumors often develop hypoxic regions, leading to aggressive behavior and increased drug resistance. The chemical composition of Cymbopogon citratus essential oil (EO) was analyzed using GC-MS. Alginate nanoparticles containing the EO its primary component, citral, were synthesized via ionic gelation method. Encapsulation confirmed ATR-FTIR analysis. anticancer efficacy C. EO, their respective alginate evaluated under normoxic (21% oxygen) (1% conditions on breast cancer (MDA-MB-231) melanoma (A-375) cell lines. Additionally, qPCR flow cytometry used assess apoptosis gene expression ratios (Bax/Bcl-2) levels apoptosis. Citral (80.98%) identified as major component EO. citral (C. citratus-AlgNPs citral-AlgNPs) with particle sizes 195 ± 4 nm 222 9 nm, zeta potentials -22 3 mV − 17 1 mV, respectively. Both samples demonstrated significantly greater conditions. had IC50 values 27 (19–39) µg/mL 25 (4-147) µg/mL, respectively, against MDA-MB-231 A-375 cells. Flow showed conditions, highest rates observed for citral-AlgNPs (84 5 92 5% in cells, respectively). This study demonstrates that enhance activity particularly highlighting potential hypoxia-targeted therapies.

Language: Английский

Biosynthesis of Edible Terpenoids: Hosts and Applications DOI Creative Commons
Mengyu Wang,

Z. Zhang,

Xinyu Liu

et al.

Foods, Journal Year: 2025, Volume and Issue: 14(4), P. 673 - 673

Published: Feb. 17, 2025

Microbial foods include microbial biomass, naturally fermented foods, and heterologously synthesized food ingredients derived from fermentation. Terpenoids, using isoprene as the basic structure, possess various skeletons functional groups. They exhibit diverse physicochemical properties physiological activities, such unique flavor, anti-bacterial, anti-oxidant, anti-cancer, hypolipemic, making them extensively used in industry, fragrance, preservatives, dietary supplements, medicinal health food. Compared to traditional strategies like direct extraction natural species chemical synthesis, cell factories for edible terpenoids have higher titers yields. can utilize low-cost raw materials are easily scaling-up, representing a novel green sustainable production mode. In this review, we briefly introduce synthetic pathway of applications producing terpenoids. Secondly, highlight several typical non-typical chassis terpenoid-producing factories. addition, reviewed recent advances representative terpenoid with gram-scale titer preservation, nutritional enhancers, foods. Finally, predict future directions their commercialization process.

Language: Английский

Citations

0
Comprehensive Review of Perilla frutescens: Chemical Composition, Pharmacological Mechanisms, and Industrial Applications in Food and Health Products DOI Creative Commons
Dandan Yi, Zhiyong Wang, Mugen Peng

et al.

Foods, Journal Year: 2025, Volume and Issue: 14(7), P. 1252 - 1252

Published: April 3, 2025

Perilla frutescens (L.) Britt., a multifunctional herbaceous plant, is widely used in traditional medicine and cuisine due to its rich array of bioactive compounds. To date, many key phytochemicals P. have been identified, including volatile terpenoids (perillaldehyde, limonene,), flavonoids (luteolin, apigenin), phenolic acids (rosmarinic acid derivatives), which exhibit significant antioxidant, anti-inflammatory, antiviral, anticancer, antibacterial, blood sugar-lowering effects. Studies shown that oils, flavonoids, exert their effects various experimental models. In food industrial applications, shows innovative potential functional foods, natural preservatives, novel additives, effectively extending shelf life providing antimicrobial protection. Moreover, research on the biology genetic improvement has provided new approaches enhance yield content. Finally, this paper also discusses safety standardization issues theoretical support for widespread application.

Language: Английский

Citations

0
Gas chromatographic techniques and spectroscopic approaches for a deep characterization of Piper gaudichaudianum Kunth essential oil from Brazil DOI Creative Commons
Lorenzo Cucinotta, Archimede Rotondo, Carmelo Coppolino

et al.

Journal of Chromatography A, Journal Year: 2024, Volume and Issue: 1732, P. 465208 - 465208

Published: July 24, 2024

Piper gaudichaudianum Kunth essential oil (EO) is a natural source of bioactive components, having multiple therapeutic applications. Its chemical composition highly variable, and strictly depends on abiotic factors, resulting in various biological activities. The present study details the utilization gas chromatographic techniques alongside nuclear magnetic resonance (NMR) spectroscopy to characterize from Brazil. Seventy-six components were identified using GC-MS analysis, while enantio‑selective multidimensional chromatography elucidated enantiomeric distribution eight chiral for first time literature. Following an unidentified component, constituting approximately 27 % total oil, prompted isolation step through preparative chromatography. Through combined use resonance, GC-Fourier transform infrared (FTIR), mass spectrometry (MS), unknown molecule was structurally as 4-[(3E)‑dec-3-en-1-yl]phenol. Remarkably, it known molecule, gibbilimbol B, not previously listed any MS database. Subsequently, spectrum included commercial library, specifically FFNSC 4.0 database, time.

Language: Английский

Citations

3
Comparison of the efficacy of alginate nanoparticles containing Cymbopogon citratus essential oil and citral on melanoma and breast cancer cell lines under normoxic and hypoxic conditions DOI Creative Commons

Farnaz Karami,

Mahmoud Osanloo, Hiva Alipanah

et al.

BMC Complementary Medicine and Therapies, Journal Year: 2024, Volume and Issue: 24(1)

Published: Oct. 19, 2024

Solid tumors often develop hypoxic regions, leading to aggressive behavior and increased drug resistance. The chemical composition of Cymbopogon citratus essential oil (EO) was analyzed using GC-MS. Alginate nanoparticles containing the EO its primary component, citral, were synthesized via ionic gelation method. Encapsulation confirmed ATR-FTIR analysis. anticancer efficacy C. EO, their respective alginate evaluated under normoxic (21% oxygen) (1% conditions on breast cancer (MDA-MB-231) melanoma (A-375) cell lines. Additionally, qPCR flow cytometry used assess apoptosis gene expression ratios (Bax/Bcl-2) levels apoptosis. Citral (80.98%) identified as major component EO. citral (C. citratus-AlgNPs citral-AlgNPs) with particle sizes 195 ± 4 nm 222 9 nm, zeta potentials -22 3 mV − 17 1 mV, respectively. Both samples demonstrated significantly greater conditions. had IC50 values 27 (19–39) µg/mL 25 (4-147) µg/mL, respectively, against MDA-MB-231 A-375 cells. Flow showed conditions, highest rates observed for citral-AlgNPs (84 5 92 5% in cells, respectively). This study demonstrates that enhance activity particularly highlighting potential hypoxia-targeted therapies.

Language: Английский

Citations

0