Serotonergic reinforcement of a complete swallowing circuit DOI Open Access
Andreas Schoofs, Anton Miroschnikow, Philipp Schlegel

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: May 26, 2023

SUMMARY How the body interacts with brain to perform vital life functions such as feeding is one of fundamental questions in physiology and neuroscience. Here, we use a whole-animal scanning transmission electron microscopy dataset Drosophila map out neuronal circuits that connect entire enteric nervous system via insect vagus nerve at synaptic resolution. This revealed periphery-brain feedback loop which Piezo-expressing mechanosensory neurons sense food intake convey information onto serotonergic within brain. These integrate interoceptive external central inputs, turn stabilize rhythmic activity serotonin receptor 7 expressing peripheral motor drive swallowing. Strikingly, very same also share an efference copy their aforementioned neurons, thereby closing motor-sensory-modulatory loop. Our analysis elucidates elemental, albeit surprisingly complex reinforcement circuit patterns are stabilized through afferent signaling upon completion rewarding action. The motif constructed allow distinction between self-generated action those response environment.

Language: Английский

Investigating local and systemic intestinal signalling in health and disease with Drosophila DOI Creative Commons

Andre Medina,

Karen Bellec, Sofía Polcowñuk

et al.

Disease Models & Mechanisms, Journal Year: 2022, Volume and Issue: 15(3)

Published: March 1, 2022

ABSTRACT Whole-body health relies on complex inter-organ signalling networks that enable organisms to adapt environmental perturbations and changes in tissue homeostasis. The intestine plays a major role as centre by producing local systemic signals are relayed the body maintain intestinal organismal Consequently, disruption of homeostasis associated with diseases multi-organ dysfunction. In recent years, fruit fly Drosophila melanogaster has emerged prime model organism study tissue-intrinsic adult due its genetic tractability functional conservation mammals. this Review, we highlight research contributed our understanding how interacts microenvironment distant organs. We discuss implications these findings for whole-body pathophysiology, future studies might advance knowledge interplay between rest disease.

Language: Английский

Citations

20
Endocrine control of glycogen and triacylglycerol breakdown in the fly model DOI
Martina Gáliková, Peter Klepsatel

Seminars in Cell and Developmental Biology, Journal Year: 2022, Volume and Issue: 138, P. 104 - 116

Published: April 5, 2022

Language: Английский

Citations

20
Generation of LexA enhancer-trap lines in Drosophila by an international scholastic network DOI Creative Commons

Ella S. Kim,

Arjun Rajan, Kathleen Chang

et al.

G3 Genes Genomes Genetics, Journal Year: 2023, Volume and Issue: 13(9)

Published: June 6, 2023

Abstract Conditional gene regulation in Drosophila through binary expression systems like the LexA-LexAop system provides a superb tool for investigating and tissue function. To increase availability of defined LexA enhancer trap insertions, we present molecular, genetic, studies 301 novel Stan-X traps derived from mobilization index SX4 line. This includes insertions into distinct loci on X, II, III chromosomes that were not previously associated with or targeted constructs, an insertion ptc, seventeen natural transposons. A subset was expressed CNS neurons known to produce secrete insulin, essential regulator growth, development, metabolism. Fly lines described here generated characterized by students teachers international network genetics classes at public, independent high schools, universities serving diversity students, including those underrepresented science. Thus, unique partnership between secondary schools university-based programs has produced resources Drosophila, establishing instructional paradigms devoted unscripted experimental

Language: Английский

Citations

12
Neuroprotective role of plumbagin on eye damage induced by high-sucrose diet in adult fruit fly Drosophila melanogaster DOI Open Access
Elisabetta Catalani,

Simona Del Quondam,

Kashi Brunetti

et al.

Biomedicine & Pharmacotherapy, Journal Year: 2023, Volume and Issue: 166, P. 115298 - 115298

Published: Aug. 17, 2023

The natural compound plumbagin has a wide range of pharmacological and potential therapeutic activities, although its role in neuroretina degeneration is unknown. Here we evaluated the effects on retina homeostasis fruit fly Drosophila melanogaster fed with high glucose diet, model hyperglycemia-induced eye impairment to study pathophysiology diabetic retinopathy at early stages. To this aim, visual system flies orally administered been analyzed structural, functional, molecular/cellular level as for instance neuronal apoptosis/autophagy dysregulation oxidative stress-related signals. Our results demonstrated that ameliorates performance hyperglycemic flies. eye-structure, clearly altered by hyperglycemia, i.e. defects pattern ommatidia, irregular rhabdomeres, vacuoles, damaged mitochondria, abnormal phototransduction units were rescued, least part, plumbagin. In addition, it reactivated autophagy, decreased presence cell death/apoptotic features, exerted antioxidant retina. terms mechanisms favoring death/survival ratio, Nrf2 signaling activation may be one strategies which reduced redox unbalance mainly increasing levels glutathione-S-transferase. Likewise, act additively and/or synergistically inhibiting mitochondrial-endoplasmic reticulum stress unfolded protein response pathways, prevented damage induced reactive oxygen species. These provide an avenue further studies, helpful develop novel candidates drug targets against neurotoxicity glucose, key aspect retinal complications diabetes.

Language: Английский

Citations

12
A nutrient responsive lipase mediates gut-brain communication to regulate insulin secretion in Drosophila DOI Creative Commons

Alka Singh,

Kandahalli Venkataranganayaka Abhilasha,

Kathya R. Acharya

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: May 23, 2024

Abstract Pancreatic β cells secrete insulin in response to glucose elevation maintain homeostasis. A complex network of inter-organ communication operates modulate secretion and regulate levels after a meal. Lipids obtained from diet or generated intracellularly are known amplify glucose-stimulated secretion, however, the underlying mechanisms not completely understood. Here, we show that Drosophila secretory lipase, Vaha (CG8093), is synthesized midgut moves brain where it concentrates insulin-producing process requiring Lipid Transfer Particle, lipoprotein originating fat body. In dietary fat, stimulates insulin-like peptide release (ILP), deficiency results reduced circulatory ILP diabetic features including hyperglycemia hyperlipidemia. Our findings suggest functions as diacylglycerol lipase physiologically, by being molecular link between lipid amplified gut-brain axis.

Language: Английский

Citations

4
Serotonergic modulation of swallowing in a complete fly vagus nerve connectome DOI Creative Commons
Andreas Schoofs, Anton Miroschnikow, Philipp Schlegel

et al.

Current Biology, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 1, 2024

Language: Английский

Citations

4
EFFICIENT RIBOSOMAL RNA DEPLETION FROM DROSOPHILA TOTAL RNA FOR NEXT-GENERATION SEQUENCING APPLICATIONS DOI Creative Commons

Omkar Koppaka,

Shweta Tandon, Ankita Chodankar

et al.

Wellcome Open Research, Journal Year: 2025, Volume and Issue: 10, P. 112 - 112

Published: Feb. 28, 2025

We developed a cost-effective enzyme-based rRNA-depletion method tailored for Drosophila melanogaster, addressing the limitations of existing commercial kits and lack peer-reviewed alternatives. Our employs single-stranded DNA probes complementary to Drosophila rRNA, forming DNA-RNA hybrids. These hybrids are then degraded using RNase H enzyme, effectively removing rRNA enriching all non-ribosomal RNAs, including mRNA, lncRNA small RNA. When compared removal kit, our approach demonstrated superior efficiency mapping percentage, confirming its effectiveness. Additionally, successfully enriched non-coding transcriptome, making it valuable tool studying ncRNA in Drosophila. The probe sequences protocol made freely available, offering reliable alternative experiments.

Language: Английский

Citations

0
Integration of Dynamical Network Biomarkers, Control Theory and Drosophila Model Identifies Vasa/DDX4 as the Potential Therapeutic Targets for Metabolic Syndrome DOI Creative Commons
Kazutaka Akagi, Yinji Jin, Keiichi Koizumi

et al.

Cells, Journal Year: 2025, Volume and Issue: 14(6), P. 415 - 415

Published: March 12, 2025

Metabolic syndrome (MetS) is a subclinical disease, resulting in increased risk of type 2 diabetes (T2D), cardiovascular diseases, cancer, and mortality. Dynamical network biomarkers (DNB) theory has been developed to provide early-warning signals the disease state during preclinical stage. To improve efficiency DNB analysis for target genes discovery, intervention based on control proposed. However, its biological validation specific such as MetS remains unexplored. Herein, we identified eight candidate from adipose tissue model mice at stage by analysis. Using Drosophila, conducted RNAi-mediated knockdown screening these vasa (also known DDX4), encoding DEAD-box RNA helicase, fat metabolism-associated gene. Fat body-specific abrogated high-fat diet (HFD)-induced enhancement starvation resistance through up-regulation triglyceride lipase. We also confirmed that DDX4 expressing adipocytes are HFD-fed high BMI patients using public datasets. These results prove potential search therapeutic targets diseases

Language: Английский

Citations

0
Inter-organ communication in Drosophila: Lipoproteins, adipokines, and immune-metabolic coordination DOI
Akhila Rajan, Jason Karpac

Current Opinion in Cell Biology, Journal Year: 2025, Volume and Issue: 94, P. 102508 - 102508

Published: April 6, 2025

Language: Английский

Citations

0
Glycolytic disruption restricts Drosophila melanogaster larval growth via the cytokine Upd3 DOI Creative Commons
Madhulika Rai, Hongde Li, Robert A. Policastro

et al.

PLoS Genetics, Journal Year: 2025, Volume and Issue: 21(5), P. e1011690 - e1011690

Published: May 2, 2025

Drosophila larval growth requires efficient conversion of dietary nutrients into biomass. Lactate dehydrogenase (Ldh) and glycerol-3-phosphate (Gpdh1) support this metabolic program by cooperatively promoting glycolytic flux. Consistent with their cooperative functions, the loss both enzymes, but not either single enzyme alone, induces a developmental arrest. However, Ldh Gpdh1 exhibit complex often mutually exclusive expression patterns, suggesting that lethal phenotypes exhibited Gpdh1; double mutants could be mediated non-autonomously. Supporting possibility, we find arrest displayed extends beyond simple disruption instead stems, in part, from changes systemic factor signaling. Specifically, demonstrate simultaneous results elevated Upd3, cytokine involved Jak/Stat Furthermore, show upd3 loss-of-function mutations suppress phenotype, indicating Upd3 signaling restricts development response to decreased Together, our findings reveal mechanism which disruptions can modulate

Language: Английский

Citations

0