Stem cell-derived hepatocyte therapy using versatile biomimetic nanozyme incorporated nanofiber-reinforced decellularized extracellular matrix hydrogels for the treatment of acute liver failure DOI Creative Commons
Yuanyuan Jin, Jiabin Zhang, Yanteng Xu

et al.

Bioactive Materials, Journal Year: 2023, Volume and Issue: 28, P. 112 - 131

Published: May 18, 2023

Reactive oxygen species (ROS)-associated oxidative stress, inflammation storm, and massive hepatocyte necrosis are the typical manifestations of acute liver failure (ALF), therefore specific therapeutic interventions essential for devastating disease. Here, we developed a platform consisting versatile biomimetic copper oxide nanozymes (Cu NZs)-loaded PLGA nanofibers [email protected] nanofibers) decellularized extracellular matrix (dECM) hydrogels delivery human adipose-derived mesenchymal stem/stromal cells-derived hepatocyte-like cells (hADMSCs-derived HLCs) (HLCs/Cu protected]/dECM). Cu could conspicuously scavenge excessive ROS at early stage ALF, reduce accumulation pro-inflammatory cytokines, herein efficiently preventing deterioration hepatocytes necrosis. Moreover, also exhibited cytoprotection effect on transplanted HLCs. Meanwhile, HLCs with hepatic-specific biofunctions anti-inflammatory activity acted as promising alternative cell source ALF therapy. The dECM further provided desirable 3D environment favorably improved hepatic functions In addition, pro-angiogenesis facilitated integration whole implant host liver. Hence, HLCs/Cu protected]/dECM performed excellent synergistic efficacy mice. This strategy using nanofiber-reinforced in situ is approach therapy shows great potential clinical translation.

Language: Английский

Macrophages in immunoregulation and therapeutics DOI Creative Commons
Shanze Chen, Abdullah F. U. H. Saeed, Quan Liu

et al.

Signal Transduction and Targeted Therapy, Journal Year: 2023, Volume and Issue: 8(1)

Published: May 22, 2023

Abstract Macrophages exist in various tissues, several body cavities, and around mucosal surfaces are a vital part of the innate immune system for host defense against many pathogens cancers. possess binary M1/M2 macrophage polarization settings, which perform central role an array tasks via intrinsic signal cascades and, therefore, must be precisely regulated. Many crucial questions about signaling modulation yet to uncovered. In addition, clinical importance tumor-associated macrophages is becoming more widely recognized as significant progress has been made understanding their biology. Moreover, they integral tumor microenvironment, playing regulation wide variety processes including angiogenesis, extracellular matrix transformation, cancer cell proliferation, metastasis, immunosuppression, resistance chemotherapeutic checkpoint blockade immunotherapies. Herein, we discuss signaling, mechanical stresses modulation, metabolic pathways, mitochondrial transcriptional, epigenetic regulation. Furthermore, have broadly extended traps essential roles autophagy aging regulating functions. discussed recent advances macrophages-mediated autoimmune diseases tumorigenesis. Lastly, targeted therapy portray prospective targets therapeutic strategies health diseases.

Language: Английский

Citations

750
Cellular and molecular mechanisms of skin wound healing DOI
Oscar A Peña, Paul Martin

Nature Reviews Molecular Cell Biology, Journal Year: 2024, Volume and Issue: 25(8), P. 599 - 616

Published: March 25, 2024

Language: Английский

Citations

306
Metabolism of tissue macrophages in homeostasis and pathology DOI Creative Commons
Stefanie K. Wculek, Gillian Dunphy, Ignacio Heras‐Murillo

et al.

Cellular and Molecular Immunology, Journal Year: 2021, Volume and Issue: 19(3), P. 384 - 408

Published: Dec. 7, 2021

Abstract Cellular metabolism orchestrates the intricate use of tissue fuels for catabolism and anabolism to generate cellular energy structural components. The emerging field immunometabolism highlights importance maintenance activities immune cells. Macrophages are embryo- or adult bone marrow-derived leukocytes that key healthy homeostasis but can also contribute pathologies such as metabolic syndrome, atherosclerosis, fibrosis cancer. Macrophage has largely been studied in vitro. However, different organs contain diverse macrophage populations specialize distinct often tissue-specific functions. This context specificity creates diverging challenges fulfill their homeostatic roles particular microenvironment conditions response pathological conditions. Here, we outline current knowledge on requirements adaptations macrophages located tissues during selected diseases.

Language: Английский

Citations

257
Challenges in Healing Wound: Role of Complementary and Alternative Medicine DOI Creative Commons

Prakash Monika,

M.N. Chandraprabha,

Annapoorni Rangarajan

et al.

Frontiers in Nutrition, Journal Year: 2022, Volume and Issue: 8

Published: Jan. 20, 2022

Although the word wound sounds like a simple injury to tissue, individual's health status and other inherent factors may make it very complicated. Hence, healing has gained major attention in healthcare. The biology is precise highly programmed, through phases of hemostasis, inflammation, proliferation remodeling. Current options for which includes, use anti-microbial agents, promoters along with application herbal natural products. However, there no efficient evidence-based therapy available specific chronic wounds that can result definitive clinical outcomes. Under co-morbid conditions, would poses numerous challenges. Use Complementary Alternative Medicines (CAMs) care sector increasing its applications management remains "separate diamonds from ore." Attempts have been made understand at molecular level, mainly analysis signature genes influence several synthetic molecules on these. We outlined review challenges role CAMs management. main focus limitations currently treatment non-healing best possible alternates consider. This information generates broader knowledge healing, be further addressed using multidisciplinary approach combination therapies.

Language: Английский

Citations

172
Mitochondrial metabolism coordinates stage-specific repair processes in macrophages during wound healing DOI Creative Commons
Sebastian Willenborg, David E. Sanin, Alexander Jaïs

et al.

Cell Metabolism, Journal Year: 2021, Volume and Issue: 33(12), P. 2398 - 2414.e9

Published: Oct. 28, 2021

Wound healing is a coordinated process that initially relies on pro-inflammatory macrophages, followed by pro-resolution function of these cells. Changes in cellular metabolism likely dictate distinct activities, but the nature changes has been unclear. Here, we profiled early- versus late-stage skin wound macrophages mice at both transcriptional and functional levels. We found glycolytic early phase not sufficient to ensure productive repair. Instead, combining conditional disruption electron transport chain with deletion mitochondrial aspartyl-tRNA synthetase, single-cell sequencing analysis, subpopulation early-stage are marked ROS (mtROS) production HIF1α stabilization, which ultimately drives pro-angiogenic program essential for timely healing. In contrast, late-phase, pro-resolving IL-4Rα-mediated respiration mitohormesis. Collectively, identify as critical control mechanism macrophage effector functions during

Language: Английский

Citations

169
The Role of Myofibroblasts in Physiological and Pathological Tissue Repair DOI Open Access
Ronen Schuster, Fereshteh S. Younesi, Maya Ezzo

et al.

Cold Spring Harbor Perspectives in Biology, Journal Year: 2022, Volume and Issue: 15(1), P. a041231 - a041231

Published: Sept. 19, 2022

Myofibroblasts are the construction workers of wound healing and repair damaged tissues by producing organizing collagen/extracellular matrix (ECM) into scar tissue. Scar tissue effectively quickly restores mechanical integrity lost architecture but comes at price functionality. Fibrotic diseases caused excessive or persistent myofibroblast activity can lead to organ failure. This review defines terminology, phenotypic characteristics, functions. We will focus on central role cell, ECM, mechanics in regulating controlling action. Additionally, we discuss how therapies based intervention potentially ameliorate outcomes. Although physiology pathology affect all organs, emphasize cutaneous hypertrophic scarring as paradigms for normal versus fibrosis. A message this is that myofibroblasts be activated from multiple cell sources, varying with local environment type injury, either restore function create an inappropriate environment.

Language: Английский

Citations

106
Role of Macrophages in Wound Healing DOI
Sebastian Willenborg, Louise Injarabian, Sabine A. Eming

et al.

Cold Spring Harbor Perspectives in Biology, Journal Year: 2022, Volume and Issue: unknown, P. a041216 - a041216

Published: Aug. 30, 2022

Monocytes/macrophages are key components of the body's innate ability to restore tissue function after injury. In most tissues, both embryo-derived tissue-resident macrophages and recruited blood monocyte-derived contribute injury response. The developmental origin injury-associated has a major impact on outcome healing process. Macrophages abundant at all stages repair coordinate progression through different phases healing. They highly plastic cells that continuously adapt their environment acquire phase-specific activation phenotypes. Advanced omics methodologies have revealed vast heterogeneity macrophage phenotypes metabolic status sites in organs. this review, we highlight role origin, link between wound state reprogramming as well fate during resolution wounding

Language: Английский

Citations

90
Nanomaterials and nanomaterials-based drug delivery to promote cutaneous wound healing DOI
Tengfei Liu, Yifei Lü, Rixing Zhan

et al.

Advanced Drug Delivery Reviews, Journal Year: 2022, Volume and Issue: 193, P. 114670 - 114670

Published: Dec. 17, 2022

Language: Английский

Citations

90
Pro-Inflammatory and Anti-Inflammatory Interleukins in Infectious Diseases: A Comprehensive Review DOI Creative Commons

Arwa A. Al-Qahtani,

Fatimah S. Alhamlan, Ahmed A. Al‐Qahtani

et al.

Tropical Medicine and Infectious Disease, Journal Year: 2024, Volume and Issue: 9(1), P. 13 - 13

Published: Jan. 4, 2024

Interleukins (ILs) are signaling molecules that crucial in regulating immune responses during infectious diseases. Pro-inflammatory ILs contribute to the activation and recruitment of cells, whereas anti-inflammatory help suppress excessive inflammation promote tissue repair. Here, we provide a comprehensive overview role pro-inflammatory diseases, with focus on mechanisms underlying their effects, diagnostic therapeutic potential, emerging trends IL-based therapies.

Language: Английский

Citations

89
Rational Design of Intelligent and Multifunctional Dressing to Promote Acute/Chronic Wound Healing DOI
Liping Zhou,

Tiantian Min,

Xiaochun Bian

et al.

ACS Applied Bio Materials, Journal Year: 2022, Volume and Issue: 5(9), P. 4055 - 4085

Published: Aug. 18, 2022

Currently, the clinic's treatment of acute/chronic wounds is still unsatisfactory due to lack functional and appropriate wound dressings. Intelligent multifunctional dressings are considered most advanced modalities. It essential design develop with required functions according microenvironment in clinical treatment. This work summarizes characteristics various common wounds, such as acute wound, diabetic burns scalded mucosal ulcers wound. Furthermore, factors transformation from chronic were analyzed. Then we focused on summarizing how researchers fully thoroughly combined complex modern technology ensure usability value dressing, photothermal-sensitive dressings, dressing (pH-sensitive ROS-sensitive osmotic pressure dressings), hemostatic guiding tissue regeneration microneedle 3D/4D printing Finally, revolutionary development transform existing into needs soon possible have carried out a reasonable meaningful outlook.

Language: Английский

Citations

81