Pore‐Environment‐Dependent Photoresponsive Oxidase‐Like Activity in Hydrogen‐Bonded Organic Frameworks

Angewandte Chemie International Edition, Journal Year: 2023, Volume and Issue: 62(13)

Published: Jan. 31, 2023

Abstract Mimicking the bioactivity of native enzymes through synthetic chemistry is an efficient means to advance biocatalysts in a cell‐free environment, however, remains long‐standing challenges. Herein, we utilize structurally explicit hydrogen‐bonded organic frameworks (HOFs) mimic photo‐responsive oxidase, and uncover important role pore environments on mediating oxidase‐like activity by constructing isostructural HOFs. We discover that HOF with suitable geometry can stabilize spatially organize catalytic substrate into favorable route, as function enzyme pocket. Based desirable activity, visual sensitive HOFs biosensor established for detection phosphatase, biomarker skeletal hepatobiliary diseases. This work demonstrates significantly influence nanozymes’ addition active center.

Language: Английский

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Photobioreactors for cultivation and synthesis: Specifications, challenges, and perspectives

Engineering in Life Sciences, Journal Year: 2021, Volume and Issue: 22(12), P. 712 - 724

Published: Oct. 27, 2021

Due to their versatility and the high biomass yield produced, cultivation of phototrophic organisms is an increasingly important field. In general, open ponds are chosen do it because economic reasons; however, this strategy has several drawbacks such as poor control culture conditions a considerable risk contamination. On other hand, photobioreactors attractive choice perform organisms, many times in large scale efficient way. Furthermore, being used bioprocesses obtain valuable chemical products. review, we briefly describe different photobioreactor set-ups, including some recent designs, characteristics. Additionally, discuss current challenges advantages that each type presents, applicability biocatalysis modern modeling tools can be applied further enhance certain process.

Language: Английский

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Enzyme immobilization in hydrogels: A perfect liaison for efficient and sustainable biocatalysis

Engineering in Life Sciences, Journal Year: 2021, Volume and Issue: 22(3-4), P. 165 - 177

Published: Dec. 21, 2021

Abstract Biocatalysis is an established chemical synthesis technology that has by no means been restricted to research laboratories. The use of enzymes for organic evolved greatly from early development proof‐of‐concept – small batch production industrial scale. Different enzyme immobilization strategies contributed this success story. Recently, the hydrogel materials attracting great interest. Within review, we pay special attention recent developments in key emerging field research. Firstly, will briefly introduce concepts both biocatalysis and worlds. Then, list interesting publications link concepts. Finally, provide outlook comment on future perspectives further exploration hydrogels.

Language: Английский

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Synthesis of Enantiopure Sulfoxides by Concurrent Photocatalytic Oxidation and Biocatalytic Reduction

Angewandte Chemie International Edition, Journal Year: 2022, Volume and Issue: 61(17)

Published: Feb. 21, 2022

Abstract The concurrent operation of chemical and biocatalytic reactions in one pot is still a challenging task, and, particular for photocatalysts, examples besides simple cofactor recycling systems are rare. However, especially due to the complementary chemistry that two fields catalysis promote, their combination has potential unlock intriguing, unprecedented overall reactivities. Herein we demonstrate reduction photocatalytic oxidation process. Specifically, enantioselective sulfoxide using ( S )‐selective methionine reductases was coupled an unselective light‐dependent sulfoxidation. Protochlorophyllide established as new green photocatalyst Overall, cyclic deracemization process produce nonracemic sulfoxides achieved target compounds were obtained with excellent conversions (up 91 %) superb optical purity (>99 % ee ).

Language: Английский

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Photobiocatalysis in Continuous Flow

Frontiers in Catalysis, Journal Year: 2022, Volume and Issue: 1

Published: Jan. 10, 2022

In the last years, there were two fields that experienced an astonishing growth within biocatalysis community: photobiocatalysis and applications of flow technology to catalytic processes. Therefore, it is not a surprise combination these research areas also gave place several recent interesting articles. However, best our knowledge, no review article covering advances was published so far. Within this review, we present very developments in field continuous flow, discuss different practical features state-of-the art photobioreactors lastly, some future perspectives field.

Language: Английский

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Exciting Enzymes: Current State and Future Perspective of Photobiocatalysis

ChemPhotoChem, Journal Year: 2023, Volume and Issue: 7(7)

Published: May 2, 2023

Abstract The recent increase of interest in photocatalysis spread to biocatalysis and triggered a rush for the development light‐dependent enzyme‐mediated or enzyme‐coupled processes. After several years intense research on photobiocatalysis, it is time evaluate state field structured manner. In this Perspective, we suggest group photobiocatalysis into distinct disciplines provide principal guidelines standards reporting photobiocatalytic results as well advice performing reactions. Overall, assess that contributes diversity biocatalytic reactions while offering selectivity enzymes photocatalysis. We foresee ongoing excitement enzymatic processes will lead discovery novel mechanisms complement with new bond‐forming additional innovative strategies utilize light possible benign energy source.

Language: Английский

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Applications of Ene-Reductases in the Synthesis of Flavors and Fragrances

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(33), P. 18305 - 18320

Published: July 5, 2024

Flavors and fragrances (F&F) are interesting organic compounds in chemistry. These widely used the food, cosmetic, medical industries. Enzymatic synthesis exhibits several advantages over natural extraction chemical preparation, including a high yield, stable quality, mildness, environmental friendliness. To date, many oxidoreductases hydrolases have been to biosynthesize F&F. Ene-reductases (ERs) class of biocatalysts that can catalyze asymmetric reduction α,β-unsaturated offer superior specificity selectivity; therefore, ERs increasingly considered an ideal alternative their counterparts. This review summarizes research progress on use F&F past 20 years, achievements various scholars, differences similarities among findings, discussions future trends related ERs. We hope this inspire researchers promote development biotechnology industry.

Language: Английский

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Flavoprotein Photochemistry: Fundamental Processes and Photocatalytic Perspectives

The Journal of Physical Chemistry B, Journal Year: 2022, Volume and Issue: 126(17), P. 3199 - 3207

Published: April 20, 2022

Flavins are highly versatile redox-active and colored cofactors in a large variety of proteins. These do include photoenzymes photoreceptors, although the vast majority performs non-light-driven physiological functions. Nevertheless, electron transfer between flavins specific nearby amino acid residues (in particular tyrosine, tryptophan, presumably histidine arginine) takes place upon excitation flavin many flavoproteins. For oxidized flavoproteins these reactions potentially have photoprotective role. In this Perspective, we outline work on characterization early reaction intermediates not only relatively well-studied resting forms but also fully reduced intrinsically unstable semireduced forms, where ultrafast photooxidation was recently demonstrated. Along different lines, flavoprotein-based novel photocatalysts for biotechnological applications presently emerging, employing both substrate photoreduction strategies. Deep insight into fundamental photochemical may help guiding optimizing their development exploration photocatalytic approaches.

Language: Английский

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Process Intensification as Game Changer in Enzyme Catalysis

Frontiers in Catalysis, Journal Year: 2022, Volume and Issue: 2

Published: March 21, 2022

Enzyme catalysis, made tremendous progress over the last years in identification of new enzymes and enzymatic reactivity’s as well optimization existing enzymes. However, performance resulting processes is often still limited, e.g., regard productivity, realized product concentrations stability Different topics (like limited specific activity, unfavourable kinetics or enzyme stability) can be addressed via engineering. On other hand, there also a long list that are not addressable by Here typical examples reaction thermodynamics, selectivity multistep reactions low water solubility. These challenges only through an adaption system. The procedures process intensification (PI) represent good approach to reach most suitable systems. general objective PI achieve significant benefits terms capital operating costs quality, waste, safety applying innovative principles. aim review show current capabilities future potentials catalysis focused on class oxidoreductases. focus paper alternative methods energy input, reactor concepts media with improved properties.

Language: Английский

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Harnessing heme chemistry: Recent advances in the biocatalytic applications of cytochrome P450 monooxgenases

Current Opinion in Green and Sustainable Chemistry, Journal Year: 2022, Volume and Issue: 39, P. 100734 - 100734

Published: Nov. 28, 2022

Language: Английский

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