Chemical Engineering & Technology,
Journal Year:
2022,
Volume and Issue:
46(4), P. 766 - 775
Published: Dec. 21, 2022
Abstract
Cofactors,
which
are
key
components
in
many
bio‐based
processes,
often
consumed
within
the
biosynthetic
reaction,
but
usually
converted
back
to
their
original
form
by
recycling.
This
study
presents
an
approach
for
selective
removal
of
cofactors
from
aqueous
solution
at
up
submillimolar
concentrations.
The
rare
earth
elements
(REE)
scandium,
yttrium,
and
lanthanum,
oxidation
state
+3,
used
sparingly
soluble
salts
with
investigated
cofactors,
pyridoxal‐5‐phosphate,
NAD
+
,
NADP
adenosine
triphosphate,
thiamine
pyrophosphate.
Up
>
99
%
efficiency
via
cofactor
salt
precipitation
was
observed.
Redissolution
recovery
formed
is
obtained
treatment
NaH
2
PO
4
a
metathesis
forming
solubilized
corresponding
REE
phosphate
salts.
Biotechnology Advances,
Journal Year:
2022,
Volume and Issue:
60, P. 108016 - 108016
Published: July 1, 2022
Fusion
proteins,
understood
as
those
created
by
joining
two
or
more
genes
that
originally
encoded
independent
have
numerous
applications
in
biotechnology,
from
analytical
methods
to
metabolic
engineering.
The
use
of
fusion
enzymes
biocatalysis
may
be
even
interesting
due
the
physical
connection
catalyzing
successive
reactions
into
covalently
linked
complexes.
proximity
active
sites
multi-enzyme
complexes
can
make
a
significant
contribution
catalytic
efficiency
reaction.
However,
does
not
guarantee
this
result.
Other
aspects,
such
nature
and
length
linker
used
for
order
which
are
fused,
must
considered
optimized
achieve
expected
increase
efficiency.
In
review,
we
will
relate
new
advances
design,
creation,
fused
with
achieved
over
past
20
years.
Thus,
discuss
some
examples
genetically
their
application
carbon‑carbon
bond
formation
oxidative
reactions,
generation
chiral
amines,
synthesis
carbohydrates,
biodegradation
plant
biomass
plastics,
preparation
other
high-value
products.
Current Opinion in Biotechnology,
Journal Year:
2022,
Volume and Issue:
78, P. 102835 - 102835
Published: Nov. 2, 2022
In
this
review,
we
focus
on
the
holistic
continuous
enzymatic
production
and
put
special
emphasis
process
intensification
by
up-
downstream
processing
in
flow
biocatalysis.
After
a
brief
introduction,
provide
an
overview
of
current
examples
enzyme
immobilization
as
upstream
for
Thereafter,
unit
operations
strategies,
namely
(i)
liquid-liquid
extraction,
(ii)
adsorptive
processing,
(iii)
crystallization
precipitation.
Eventually,
present
our
perspectives
future
trends
research
field.
Foods,
Journal Year:
2023,
Volume and Issue:
12(13), P. 2518 - 2518
Published: June 28, 2023
As
the
plant-based
food
market
grows,
demand
for
plant
protein
is
also
increasing.
Proteins
are
a
major
component
in
foods
and
key
to
developing
desired
structures
textures.
Seed
storage
proteins
main
human
diet.
They
abundant
in,
example,
legumes
or
defatted
oilseeds,
which
makes
them
an
excellent
candidate
use
development
of
novel
foods.
However,
they
often
have
low
inflexible
functionalities,
as
nature
designed
remain
densely
packed
inert
within
cell
walls
until
needed
during
germination.
Enzymes
used
by
industry,
production
cheese
beer,
modify
ingredient
properties.
Although
currently
limited
applications
proteins,
interest
area
exponentially
The
present
review
first
considers
current
state
potential
enzyme
utilization
related
including
uses
extraction
post-extraction
modifications.
Then,
relevant
opportunities
challenges
critically
discussed.
relate
knowledge
gap,
high
cost
enzymes,
complexity
substrates.
overall
aim
this
increase
awareness,
highlight
challenges,
explore
ways
address
them.
RSC Sustainability,
Journal Year:
2024,
Volume and Issue:
2(3), P. 608 - 615
Published: Jan. 1, 2024
What
can
we
do
with
Deep
Eutectic
Solvents
(DES)
once
they
have
been
used?
be
their
disposal
and
final
fate?
This
perspective
adds
some
considerations
to
stimulate
the
debate.
Molecules,
Journal Year:
2023,
Volume and Issue:
28(18), P. 6452 - 6452
Published: Sept. 6, 2023
Biocatalysis
can
be
applied
in
aqueous
media
and
different
non-aqueous
solutions
(non-conventional
media).
Water
is
a
safe
solvent,
yet
many
synthesis-wise
interesting
substrates
cannot
dissolved
solutions,
thus
low
concentrations
are
often
applied.
Conversely,
non-conventional
may
enable
higher
substrate
loadings
but
at
the
cost
of
using
(fossil-based)
organic
solvents.
This
paper
determines
CO2
production-expressed
as
kg
CO2·kg
product-1-of
generic
biotransformations
water
media,
assessing
both
upstream
downstream.
The
key
to
reaching
diminished
environmental
footprint
type
wastewater
treatment
implemented.
If
used
chemicals
conventional
(mild)
treatment,
production
limited.
other
(pre)treatments
for
needed
eliminate
hazardous
solvents,
impacts
expected
(based
on
production).
biocatalysis
more
sustainable
during
unit-the
biocatalytic
step-than
systems.
However,
processes
with
need
incorporate
extractive
solvents
downstream
processing.
Both
strategies
result
comparable
if
recycled
least
1-2
times.
Under
these
conditions,
industrial
biotransformation
100
g
L-1
loading
would
produce
15-25
product-1
regardless
media.
Applied Food Research,
Journal Year:
2023,
Volume and Issue:
4(1), P. 100363 - 100363
Published: Nov. 19, 2023
Process
Intensification
(PI)
is
a
cutting-edge
processing
technology
that
uses
ground-breaking
methods
to
produce
goods
more
efficiently,
successfully,
and
with
superior
quality
while
consuming
less
energy
resources.
It
strategy
gaining
popularity
for
boosting
the
effectiveness
of
industrial
activities.
The
main
objective
PI
technique
hasten,
simplify,
reduce
expense
operational
procedures.
In
food
business,
process
intensification
has
been
recognized
as
significant
tactic
producing
can
be
considered
both
sustainable
healthy.
might
water
use
also
raising
caliber
finished
product.
applied
sector
improve
variety
characteristics,
including
flavor,
texture,
shelf
life,
nutrient
content,
safety.
Establishing
efficient
cost-effective
processes
time,
effort,
well
additional
resources
fewer
byproducts
made
possible
by
PI.
Utilizing
this
method
promote
ecologically
friendly
lower
carbon
emissions.
This
evaluation
will
concentrate
on
how
significantly
increased
production
efficiency
allowing
contamination
waste
reduction
through
application
contemporary
techniques.
EFB Bioeconomy Journal,
Journal Year:
2023,
Volume and Issue:
3, P. 100056 - 100056
Published: June 7, 2023
Biocatalysis
has
matured
to
be
used
in
an
ample
array
of
reaction
media,
from
pure
aqueous
solutions
(water-free)
non-conventional
media.
In
most
the
applications
solvents
are
needed
as
media
and/or
during
downstream
unit
extract
and
purify
product.
Aligning
processes
with
Green
Chemistry
principles,
some
green
have
been
introduced.
This
article
discusses
critically
environmental
impact
that
may
a
biocatalytic
reaction,
raw
material
extraction
solvent
synthesis
its
use
biocatalysis
ultimate
disposal.
Some
observed
impacts
–
quantified
kg
CO2·kg
product−1
surely
unavoidable,
they
depend
on
or
used.
However,
intensification
(e.g.
using
higher
substrate
loadings)
investing
efforts
recycling
clear
final
ecological
footprint
enzymatic
process.
Instead
qualitatively
stating
used,
experiments
these
directions
should
performed
provide
conditions
minimized
metrics
for
sustainable
chemistry.
