Unveiling the Genetic Symphony: Harnessing CRISPR-Cas Genome Editing for Effective Insect Pest Management

Plants, Journal Year: 2023, Volume and Issue: 12(23), P. 3961 - 3961

Published: Nov. 24, 2023

Phytophagous insects pose a significant threat to global crop yield and food security. The need for increased agricultural output while reducing dependence on harmful synthetic insecticides necessitates the implementation of innovative methods. utilization CRISPR-Cas (Clustered regularly interspaced short palindromic repeats) technology develop insect pest-resistant plants is believed be highly effective approach in production expenses enhancing profitability farms. Insect genome research provides vital insights into gene functions, allowing better knowledge biology, adaptability, development targeted pest management disease prevention measures. editing technique has capability modify DNA insects, either trigger drive or overcome their resistance specific insecticides. advancements CRISPR its various applications have shown potential developing insect-resistant varieties other strategies through sustainable approach. This could consequences ensuring involves using create modified plants. article critically analyzed discussed challenges associated with exploring utilizing pressure

Language: Английский

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Screening and Functional Analysis of a Novel Salivary Effector DcE13 from the Asian Citrus Psyllid, Diaphorina citri

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 2, 2025

The lack of sequence similarity among insect effectors necessitates the independent identification in Asian citrus psyllid Diaphorina citri, primary vector huanglongbing pathogens. In this study, nine candidate were identified from D. citri salivary gland transcriptome. Among them, DcE13 exhibited highest FPKM value and was delivered into plants during feeding. Transient expression tobacco leaves showed that is localized plasma membrane around nuclear envelope plant cells. It inhibits BAX- INF1-induced hypersensitive responses, suppresses callose deposition, downregulates jasmonic acid marker genes NtPDF1.2 NtFAD7. Furthermore, knockdown decreased survival rates fecundity as well interrupted their phloem feeding, indicating its role an effector facilitates These results enrich our current understanding feeding behavior while simultaneously offering a potential target gene for controlling pest.

Language: Английский

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A limited concentration range of diaphorin, a polyketide produced by a bacterial symbiont of the Asian citrus psyllid, promotes thein vitrogene expression with bacterial ribosomes

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 5, 2024

Abstract Diaphorin is a polyketide produced by “ Candidatus Profftella armatura” ( Gammaproteobacteria : Burkholderiales ), an obligate symbiont of devastating agricultural pest, the Asian citrus psyllid Diaphorina citri (Hemiptera: Psyllidae). Physiological concentrations diaphorin, which D. contains at levels as high 2–20 mM, are inhibitory to various eukaryotes and Bacillus subtilis Firmicutes Bacilli ) but promote growth metabolic activity Escherichia coli Enterobacterales ). Our previous study demonstrated that five-millimolar exhibits significant promoting effects on cultured B. E. , respectively, inhibits in vitro gene expression utilizing purified ribosomes. This suggested adverse diaphorin partly due its influence expression. However, result appeared inconsistent with positive . Moreover, concentration bacterial cells, where genes expressed vivo may be lower than culture media. Therefore, present analyzed 50 500 μM using same analytical method. The revealed this range contrast promotes translation ribosomes, suggesting direct translation. for first time pederin-type compound expression, establishing basis potential pest management industrial applications. Importance limited secondary metabolite cell-free substrates proteins from bacteria. unique property affect microbial flora insect, potentially influencing transmission plant pathogens. exploited improve efficacy production often used produce important materials, including pharmaceuticals, enzymes, amino acids, biofuels. elucidated part mechanism expressed, constructing foundation applying use.

Language: Английский

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A limited concentration range of diaphorin, a polyketide produced by a bacterial symbiont of the Asian citrus psyllid, promotes the in vitro gene expression with bacterial ribosomes

Microbiology Spectrum, Journal Year: 2024, Volume and Issue: 12(7)

Published: June 4, 2024

ABSTRACT Diaphorin is a polyketide produced by “ Candidatus Profftella armatura” ( Gammaproteobacteria: Burkholderiales ), an obligate symbiont of devastating agricultural pest, the Asian citrus psyllid Diaphorina citri (Hemiptera: Psyllidae). Physiological concentrations diaphorin, which D. contains at levels as high 2–20 mM, are inhibitory to various eukaryotes and Bacillus subtilis Firmicutes: Bacilli ) but promote growth metabolic activity Escherichia coli Enterobacterales ). Our previous study demonstrated that 5-mM exhibits significant promoting effects on cultured B. E. , respectively, inhibits in vitro gene expression utilizing purified ribosomes. This suggested adverse diaphorin partly due its influence expression. However, result appeared inconsistent with positive impact . Moreover, concentration bacterial cells, where genes expressed vivo may be lower than culture media. Therefore, present analyzed 50 500 µM using same analytical method. The revealed this range contrast promotes translation ribosomes, suggesting direct translation. for first time pederin-type compound expression, establishing basis potential pest management industrial applications. IMPORTANCE limited secondary metabolite cell-free substrates proteins from bacteria. unique property affect microbial flora insect, potentially influencing transmission plant pathogens. exploited improve efficacy production coli, often used produce important materials, including pharmaceuticals, enzymes, amino acids, biofuels. elucidated part mechanism expressed, constructing foundation applying distinct use.

Language: Английский

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El impacto de Huanglongbing, HLB, en la producción mundial de cítricos

Revista Boliviana de Química, Journal Year: 2024, Volume and Issue: 41(1)

Published: April 29, 2024

El cultivo de naranja se originó en el hemisferio oriental, principalmente China, y luego extendió por todo mundo como fruta comercial. En 2022, la producción mundial naranjas aumentó a un total 49 millones toneladas métricas. Los principales productores incluyen Brasil, UE, México Estados Unidos. Sin embargo, industria los cítricos enfrenta desafíos debido enfermedad Huanglongbing (HLB). investigadores están explorando modificaciones genéticas para lograr tolerancia. Esta revisión explora impacto enfermedad, las especies importancia, variedades mejoradas evolución global del HLB.

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Risk-based regionalization approach for area-wide management of HLB vectors in the Mediterranean Basin

Frontiers in Plant Science, Journal Year: 2023, Volume and Issue: 14

Published: Dec. 4, 2023

Huanglongbing (HLB) is one of the most devastating citrus diseases worldwide. It associated with non-culture bacteria Candidatus Liberibacter spp., which can be transmitted by grafting and/or psyllid vectors Diaphorina citri (ACP) and Trioza erytreae (AfCP). Although HLB has not been reported in Mediterranean Basin to date, both are present, thus represent a serious threat industry this region. Resistant cultivars or effective therapeutic treatments currently available for HLB. Nevertheless, area-wide pest management via coordinated efforts over large areas implemented Brazil, China USA control. This study proposes an open access flexible methodology address Basin. Based on risk-based approach considers climatic information other variables that may influence vector introduction spread, such as conventional, organic, abandoned residential well transportation corridors, division (PMAs) proposed. The size location these PMAs were estimated means hierarchical clustering algorithm spatial constraints whose performance was assessed under different configuration scenarios. proposal assist policymakers citrus-growing risk planning case spread possible disease. Additionally, it valuable resource inform opinion dynamic models, enabling identification pivotal factors success control measures.

Language: Английский

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Evaluation of Artemisia absinthium L. Essential Oil as a Potential Novel Prophylactic against the Asian Citrus Psyllid Diaphorina citri Kuwayama

Horticulturae, Journal Year: 2023, Volume and Issue: 9(7), P. 758 - 758

Published: July 1, 2023

Interest in developing novel crop protectants has increased the recent decade due to harmful effects of synthetic pesticides on humans and environment. Diaphorina citri threatens citrus industry worldwide is primary vector phloem-limited bacterium (HLB). However, there no available cure for HLB. management mainly depends use insecticides, but their massive application leads resistance pest populations. Therefore, alternative strategies are needed. Our results indicated that fewer D. adults settled plants treated with AAEO than control 48 h after release. The psyllids fed leaves significantly reduced honeydew production compared control. showed potent ovicidal activity against eggs LC50 5.88 mg/mL. Furthermore, we also explored fitness AAEO-treated untreated Citrus sinensis by using two-sex life table tools. revealed intrinsic rate increase (r) was higher seedlings (0.10 d−1) those an LC20 concentration (0.07 d−1). Similarly, net reproductive (R0) (14.21 offspring) (6.405 offspring). were safer Aphis mellifera, 35.05 mg/mL, which relatively 24.40 mg/mL values citri. indicate exhibits toxic behavioral citri, can be a potential candidate managing this pest.

Language: Английский

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Pests and resistance: The biology and control of supervectors and superpests

Current Opinion in Insect Science, Journal Year: 2023, Volume and Issue: 58, P. 101060 - 101060

Published: May 23, 2023

Language: Английский

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Host plant-induced changes in metabolism and osmotic regulation gene expression in Diaphorina citri adults

Journal of Insect Physiology, Journal Year: 2023, Volume and Issue: 152, P. 104599 - 104599

Published: Dec. 9, 2023

Language: Английский

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Vesicular transport-related genes in Diaphorina citri are involved in the process of Candidatus Liberibacter asiaticus infection

Journal of Integrative Agriculture, Journal Year: 2024, Volume and Issue: 23(12), P. 4136 - 4146

Published: March 22, 2024

Asian citrus psyllid (ACP, Diaphorina citri) is the major vector of Candidatus Liberibacter asiaticus (CLas), which a bacterial pathogen causing devastating Huanglongbing (HLB) disease. citri known to carry CLas in persistent and propagative manner. Some studies have suggested that may use vesicular structures D. cells as its propagation organelles. However, mechanisms by enters how vesicle-mediated trafficking involved remain unclear. In this study, we monitored titer change nymphs during process acquisition from feeding on infected plants. We found increased with access period. After infection, there was significant upregulation expression several transport-related genes citri. The significantly reduced midgut whole insect body when endocytosis endosome network were inhibited. Furthermore, silencing clathrin-heavy chain gene also led reduction These results suggest infection upregulates related transport citri, facilitates invasion endocytosis-dependent pathogens.

Language: Английский

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