Molecular characterization of a novel thioredoxin‐related transmembrane protein gene AcTMX3 that plays important roles in antioxidant defence in Arma chinensis diapause DOI

Qiaozhi Luo,

Jianjun Mao, Yuyan Li

et al.

Insect Molecular Biology, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 23, 2024

Abstract Protein disulphide isomerase (PDI) possesses isomerase, oxidoreductase and molecular chaperone activities, is involved in regulating various physiological processes. However, there are few studies on the function insect diapause. In this study, we cloned one novel member PDI family (TMX3, thioredoxin‐related transmembrane protein 3) Arma chinensis . The AcTMX3 encodes 426 amino acids that contains a predicted N‐terminal signal sequence, thioredoxin‐like domain with CXXC active site potential region, which typical sequence features of TMX3. RT‐qPCR results showed was mainly expressed head under non‐diapause conditions, while highly fat body (central metabolic organ) diapause conditions. Moreover, temporal expression profile compared conditions significantly induced expression, enhanced at 15°C. Silencing A. inhibited antioxidant genes ( AcTrx2 AcTrx‐like ), increased content H 2 O ascorbate reduced survival rate Our suggested played an important role resistance to oxidative stress

Language: Английский

Transcriptome and Neuroendocrinome Responses to Environmental Stress in the Model and Pest Insect Spodoptera frugiperda DOI Open Access

Wei Gong,

Jan Lubawy, Paweł Marciniak

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(2), P. 691 - 691

Published: Jan. 15, 2025

The fall armyworm, Spodoptera frugiperda, is one of the most notorious pest insects, causing damage to more than 350 plant species, and feared worldwide as an invasive species since it exhibits high adaptivity against environmental stress. Here, we therefore investigated its transcriptome responses four different types stresses, namely cold, heat, no water food. We used brain samples our interest was in neuroendocrine responses, while previous studies whole bodies larvae or moths. In general, were complex encompassed a vast array neuropeptides (NPs) biogenic amines (BAs). NPs mainly involved ion homeostasis regulation (ITP ITPL) metabolic pathways (AKH, ILP), this accompanied by changes BA (DA, OA) biosynthesis. Cold no-water stress changed NP gene expression with same patterns but clearly separated from each other, divergent pattern shown after no-food conclusion, data provide foundation important model insect candidate BAs other marker genes response stress, also potential new targets manage insects.

Language: Английский

Citations

1
Cross-talk between low temperature and other environmental factors DOI
Leigh Boardman

Current Opinion in Insect Science, Journal Year: 2024, Volume and Issue: 63, P. 101193 - 101193

Published: March 13, 2024

Language: Английский

Citations

5
Sub-lethal pesticide exposure facilitates the potential northward range shifts of ticks by increasing cold tolerance and overwintering survival DOI Creative Commons
Kennan Oyen,

Thomas Arya,

B H Davies

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: April 19, 2025

Abstract Pesticides are a primary tool for the control of harmful insects or other organisms throughout world. Although most likely to encounter pesticides in environment, little is known about effects these chemicals on physiology off-target species. We measured impact sublethal pesticide exposure cold tolerance two common tick predicted that would make ticks more sensitive extreme temperatures. In contrast, we found had opposite effect, increasing survival at low Sublethal decreased LT50 (lethal temperature which 50% mortality observed) adult and nymphal D. variabilis from −16°C −19°C improved overwintering survival. Evaluation previously available RNA-seq data between rapid hardening indicated potential transcriptional shifts associated with cross-tolerance exposure. To investigate population-level impacts this physiological shift tolerance, developed novel approach incorporating into spatially explicit species distribution models (SDMs). Using approach, demonstrate pesticide-induced increases may permit faster northward range . also SDMs moderates estimated environmental change provide accurate predictions responses changing environments. our study limited ticks, studies have shown thermal traits diverse The extensive use drive complex interactions their environments, leading altered establishment new habitats.

Language: Английский

Citations

0
Molecular identification of two thioredoxin genes and their function in antioxidant defense in Arma chinensis diapause DOI Creative Commons
Zhongjian Shen,

Qiaozhi Luo,

Jianjun Mao

et al.

Frontiers in Physiology, Journal Year: 2024, Volume and Issue: 15

Published: July 24, 2024

Thioredoxin (Trx), an important part of thioredoxin systems, plays crucial role in maintaining the intracellular redox balance by scavenging reactive oxygen species (ROS). However, few Trxs have been functionally characterized

Language: Английский

Citations

2
Dietary potassium and cold acclimation additively increase cold tolerance in Drosophila melanogaster DOI Creative Commons

Bassam Helou,

Marshall W. Ritchie, Heath A. MacMillan

et al.

Journal of Insect Physiology, Journal Year: 2024, Volume and Issue: 159, P. 104701 - 104701

Published: Sept. 7, 2024

Language: Английский

Citations

1
Seasonal Patterns in the Frequency of Candidatus Liberibacter Asiaticus in Populations of Diaphorina citri (Hemiptera: Psyllidae) in Florida DOI Creative Commons
Timothy A. Ebert,

Dalia Shawer,

R. H. Brlansky

et al.

Insects, Journal Year: 2023, Volume and Issue: 14(9), P. 756 - 756

Published: Sept. 11, 2023

Candidatus Liberibacter asiaticus (CLas) is one of the putative causal agents huanglongbing, which a serious disease in citrus production. The pathogen transmitted by Diaphorina citri Kuwayama (Hemiptera: Psyllidae). As an observational study, six groves central Florida and grove at southern tip were sampled monthly from January 2008 through February 2012 (50 months). collected psyllids sorted sex abdominal color. Disease prevalence adults peaked November, with minor peak February. Gray/brown females had highest prevalence, blue/green individuals either lowest prevalence. CLas was highly correlated other sexes colors. Thus, underlying causes for seasonal fluctuations operated similar fashion all psyllids. pattern caused larger nymphs displacing smaller ones optimal feeding sites immunological robustness different sex–color morphotypes. Alternative hypotheses also considered. Improving our understanding biological interactions how to sample them will improve management decisions. We agree authors that psyllid critical year-round.

Language: Английский

Citations

3
Dietary potassium and cold acclimation additively increase cold tolerance in Drosophila melanogaster DOI

Bassam Helou,

Marshall W. Ritchie, Heath A. MacMillan

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: May 28, 2024

Abstract In the cold, chill susceptible insects lose ability to regulate ionic and osmotic gradients. This leads hemolymph hyperkalemia that drives a debilitating loss of cell membrane polarization, triggering death pathways causing organismal injury. Biotic abiotic factors can modulate insect cold tolerance by impacting mitigate or prevent this cascade events. present study, we test combined isolated effects dietary manipulations thermal acclimation on in fruit flies. Specifically, acclimated adult Drosophila melanogaster 15 25°C fed them either K + -loaded diet control diet. We then tested these flies recover from survive exposure, as well their capacity protect transmembrane gradients, intracellular Na concentration. As predicted, cold-exposed experienced cold-acclimated had improved due an maintenance concentration at low temperature. Feeding high-K additively, but paradoxically reduced maintain extracellular concentrations. Cold-acclimation -feeding additively increased concentration, aiding gradient during exposure despite cold-induced hyperkalemia. There was no effect These findings suggest loading muscle sensitivity mechanisms through which -fed are able tolerate Highlights - Insect varies relation ionoregulatory Cold improves handling A high also tolerance, reduces -handling highlight novel mechanism for preventing disruption

Language: Английский

Citations

0
Editorial Overview: Insect cold tolerance research reaches a Swift new Era DOI
Nicholas M. Teets, Heath A. MacMillan

Current Opinion in Insect Science, Journal Year: 2024, Volume and Issue: unknown, P. 101284 - 101284

Published: Oct. 1, 2024

Language: Английский

Citations

0
Molecular characterization of a novel thioredoxin‐related transmembrane protein gene AcTMX3 that plays important roles in antioxidant defence in Arma chinensis diapause DOI

Qiaozhi Luo,

Jianjun Mao, Yuyan Li

et al.

Insect Molecular Biology, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 23, 2024

Abstract Protein disulphide isomerase (PDI) possesses isomerase, oxidoreductase and molecular chaperone activities, is involved in regulating various physiological processes. However, there are few studies on the function insect diapause. In this study, we cloned one novel member PDI family (TMX3, thioredoxin‐related transmembrane protein 3) Arma chinensis . The AcTMX3 encodes 426 amino acids that contains a predicted N‐terminal signal sequence, thioredoxin‐like domain with CXXC active site potential region, which typical sequence features of TMX3. RT‐qPCR results showed was mainly expressed head under non‐diapause conditions, while highly fat body (central metabolic organ) diapause conditions. Moreover, temporal expression profile compared conditions significantly induced expression, enhanced at 15°C. Silencing A. inhibited antioxidant genes ( AcTrx2 AcTrx‐like ), increased content H 2 O ascorbate reduced survival rate Our suggested played an important role resistance to oxidative stress

Language: Английский

Citations

0