First evidence of the A302S Rdl insecticide resistance mutation in populations of the bed bug, Cimex lectularius (Hemiptera: Cimicidae) in North America

Journal of Medical Entomology, Journal Year: 2025, Volume and Issue: unknown

Published: March 14, 2025

Abstract The common bed bug, Cimex lectularius (L.) (Hemiptera: Cimicidae), is a pervasive indoor pest with prominent medical, veterinary, and economic impacts. Bed bug infestations are controlled by wide range of insecticides, including pyrethroids, neonicotinoids, pyrroles, phenylpyrazoles; however, bugs have evolved resistance mechanisms to most these insecticides. Mutations in the Rdl (resistance dieldrin) gene, located subunit γ-amino butyric acid (GABA)-gated chloride channel, been identified several insects, German cockroach. These found confer fipronil, phenylpyrazole insecticide commonly used urban environments, addition cyclodienes (eg dieldrin), class insecticides banned countries since 1990s. While dieldrin fipronil has reported bugs, both C. tropical hemipterus, occurrence mutations gene yet be thoroughly investigated. In this study, we sequence fragment harbor cyclodiene conferring from 134 unique populations collected across United States Canada spanning 14-yr period. Homozygous genotypes for A302S mutation were 2 geographically distinct populations. This finding represents first record non-synonymous identifies another mechanism which may conferred species.

Language: Английский

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Target site mutations and metabolic detoxification of insecticides in continental populations of Cimex lectularius and Cimex hemipterus (Hemiptera: Cimicidae)

Journal of Medical Entomology, Journal Year: 2024, Volume and Issue: 62(1), P. 130 - 145

Published: Oct. 22, 2024

Abstract In recent decades, the common and tropical bed bugs have experienced a resurgence in many parts of world. The evolution insecticide resistance bug populations is considered significant factor contributing to this resurgence. We analyzed samples Cimex lectularius L. hemipterus (F.) from Europe (Spain 41, Switzerland 2, Czech Republic 1), Asia (Hong Kong 34), North America (USA 14, Mexico 3), South (Colombia 3) assess prevalence mechanisms resistance. identified specimens morphologically barcoded them by sequencing mitochondrial Cytochrome c oxidase subunit I (COI) 16S ribosomal RNA (16S rRNA) genes. Additionally, we screened segments voltage-gated sodium channel (VGSC) nicotinic acetylcholine receptor (nAChR) genes for point mutations associated with measured activity detoxifying enzymes. All were as C. lectularius, whereas Hong hemipterus. Out 64 tested knockdown (kdr) mutations, 90.6% contained at least 1 known mutation. 35 exhibited kdr mutations. A new mutation was pyrethroid target site both (F1524C) (F1450C) bugs. No resistance-associated nAChR gene found. Several that also showed elevated high frequency kdr-associated Spain limits efficacy pyrethroids their control.

Language: Английский

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Editorial overview: Insecticide resistance mechanisms — from behavior and physiology to microbiome science

Current Opinion in Insect Science, Journal Year: 2024, Volume and Issue: 63, P. 101204 - 101204

Published: May 8, 2024

Language: Английский

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