Long-lasting
forms
of
postsynaptic
plasticity
commonly
involve
protein
synthesis-dependent
structural
changes
dendritic
spines.
However,
the
relationship
between
synthesis
and
presynaptic
remains
unclear.
Here,
we
investigated
in
cannabinoid-receptor
1
(CB
)-mediated
long-term
depression
inhibitory
transmission
(iLTD),
a
form
that
involves
protein-synthesis-dependent
long-lasting
reduction
GABA
release.
We
found
CB
-iLTD
acute
rat
hippocampal
slices
was
associated
with
changes.
Using
proteomics,
determined
activation
neurons
resulted
increased
ribosomal
proteins
initiation
factors,
but
decreased
levels
involved
regulation
actin
cytoskeleton,
such
as
ARPC2
WASF1/WAVE1,
Moreover,
while
ubiquitin/proteasome
activity,
ubiquitination
not
proteasomal
degradation
critical
for
functional
-iLTD.
Thus,
relies
on
both
to
elicit
underlie
Proceedings of the National Academy of Sciences,
Journal Year:
2021,
Volume and Issue:
118(13)
Published: March 26, 2021
Significance
Local
protein
synthesis
is
important
for
neuronal
function
and
synaptic
plasticity.
Thousands
of
mRNAs
are
found
in
axons
dendrites,
it
believed
that
regulating
their
dynamic
transport
distribution
a
key
determinant
where
when
proteins
made.
In
this
work
we
quantitatively
assessed
the
three
synaptically
localized
live
cultured
neurons
without
exogenous
stimulation
following
induction
two
distinct
forms
Coupling
observations
mRNA
dynamics
with
imaging
endogenous
dynamics,
alterations
movements
occur
independently
translational
state,
indicating
multistep
mechanism
capture
decoding
an
to
determine
translation
occurs.
Molecular Cell,
Journal Year:
2023,
Volume and Issue:
83(24), P. 4509 - 4523.e11
Published: Dec. 1, 2023
The
cytoplasm
is
highly
compartmentalized,
but
the
extent
and
consequences
of
subcytoplasmic
mRNA
localization
in
non-polarized
cells
are
largely
unknown.
We
determined
enrichment
TIS
granules
(TGs)
rough
endoplasmic
reticulum
(ER)
through
particle
sorting
isolated
cytosolic
mRNAs
by
digitonin
extraction.
When
focusing
on
genes
that
encode
non-membrane
proteins,
we
observed
52%
have
transcripts
enriched
specific
compartments.
Compartment
correlates
with
a
combinatorial
code
based
length,
exon
3′
UTR-bound
RNA-binding
proteins.
Compartment-biased
differ
functional
classes
their
encoded
proteins:
TG-enriched
low-abundance
proteins
strong
transcription
factors,
whereas
ER-enriched
large
expressed
an
important
determinant
protein
abundance,
which
supported
reporter
experiments
showing
redirecting
to
ER
increases
expression.
In
summary,
functionally
compartmentalized
local
translation
environments.
