Application of modern synthetic biology technology in aromatic amino acids and derived compounds biosynthesis DOI
Mi Tang,

Jiajia You,

Tianjin Yang

et al.

Bioresource Technology, Journal Year: 2024, Volume and Issue: 406, P. 131050 - 131050

Published: June 26, 2024

Language: Английский

Dynamic regulation of ppGpp under a quorum sensing-based promoter to improve the toyocamycin production DOI
Yijie Wang, Hai Jin, Xiaoping Yu

et al.

Food Bioscience, Journal Year: 2025, Volume and Issue: unknown, P. 106459 - 106459

Published: March 1, 2025

Language: Английский

Citations

0
Microbial Transcription Factor-Based Biosensors: Innovations from Design to Applications in Synthetic Biology DOI Creative Commons

Kyeongseok Song,

Hyunju Ji, Ji‐Won Lee

et al.

Biosensors, Journal Year: 2025, Volume and Issue: 15(4), P. 221 - 221

Published: March 31, 2025

Transcription factor-based biosensors (TFBs) are powerful tools in microbial biosensor applications, enabling dynamic control of metabolic pathways, real-time monitoring intracellular metabolites, and high-throughput screening (HTS) for strain engineering. These systems use transcription factors (TFs) to convert metabolite concentrations into quantifiable outputs, precise regulation fluxes biosynthetic efficiency cell factories. Recent advancements TFB, including improved sensitivity, specificity, range, have broadened their applications synthetic biology industrial biotechnology. Computational such as Cello further revolutionized TFB design, silico optimization construction complex genetic circuits integrating multiple signals achieving gene regulation. This review explores innovations biosensors, role engineering adaptive evolution, future integration with artificial intelligence advanced technologies overcome critical challenges bioproduction.

Language: Английский

Citations

0
Establishing an Autonomous Cascaded Artificial Dynamic (AutoCAD) regulation system for improved pathway performance DOI Creative Commons
Tian Jiang, Chenyi Li, Yusong Zou

et al.

Metabolic Engineering, Journal Year: 2022, Volume and Issue: 74, P. 1 - 10

Published: Aug. 27, 2022

Language: Английский

Citations

17
Concentration Recognition‐Based Auto‐Dynamic Regulation System (CRUISE) Enabling Efficient Production of Higher Alcohols DOI Creative Commons
Zhenya Chen, Shengzhu Yu, Jing Liu

et al.

Advanced Science, Journal Year: 2024, Volume and Issue: 11(23)

Published: April 16, 2024

Abstract Microbial factories lacking the ability of dynamically regulating pathway enzymes overexpression, according to in situ metabolite concentrations, are suboptimal, especially when metabolic intermediates competed by growth and chemical production. The production higher alcohols (HAs), which hijacks amino acids (AAs) from protein biosynthesis, minimizes intracellular concentration AAs thus inhibits host growth. To balance resource allocation maintain stable AA flux, this work utilizes AA‐responsive transcriptional attenuator ivbL HA‐responsive activator BmoR establish a recognition‐based auto‐dynamic regulation system (CRUISE). This ultimately maintains homeostasis maximizes HA. It is demonstrated that ‐driven overexpression can regulate AA‐to‐HA conversion while BmoR‐driven accelerate biosynthesis during HA feedback activation mode. flux pathways balanced via concentration, vice versa stabilized competition between conversion. CRUISE, further aided scaffold‐based self‐assembly, enables 40.4 g L −1 isobutanol bioreactor. Taken together, CRUISE realizes robust sheds new light on dynamic control process

Language: Английский

Citations

3
Application of modern synthetic biology technology in aromatic amino acids and derived compounds biosynthesis DOI
Mi Tang,

Jiajia You,

Tianjin Yang

et al.

Bioresource Technology, Journal Year: 2024, Volume and Issue: 406, P. 131050 - 131050

Published: June 26, 2024

Language: Английский

Citations

3