Engineering Metabolic Flux for the Microbial Synthesis of Aromatic Compounds DOI
Zhendong Li,

Xianghe Wang,

Guipeng Hu

et al.

Metabolic Engineering, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 1, 2024

Language: Английский

Relieving metabolic burden to improve robustness and bioproduction by industrial microorganisms DOI Creative Commons
Jiwei Mao, Hongyu Zhang, Yu Chen

et al.

Biotechnology Advances, Journal Year: 2024, Volume and Issue: 74, P. 108401 - 108401

Published: June 27, 2024

Metabolic burden is defined by the influence of genetic manipulation and environmental perturbations on distribution cellular resources. The rewiring microbial metabolism for bio-based chemical production often leads to a metabolic burden, followed adverse physiological effects, such as impaired cell growth low product yields. Alleviating imposed undesirable changes has become an increasingly attractive approach constructing robust factories. In this review, we provide brief overview engineering, focusing specifically recent developments strategies diminishing while improving robustness yield. A variety examples are presented showcase promise engineering in facilitating design construction Finally, challenges limitations encountered discussed.

Language: Английский

Citations

24
Genetic circuits for metabolic flux optimization DOI
Xianhao Xu, Xueqin Lv,

Xinyu Bi

et al.

Trends in Microbiology, Journal Year: 2024, Volume and Issue: 32(8), P. 791 - 806

Published: March 12, 2024

Language: Английский

Citations

8
The expanded CRISPR toolbox for constructing microbial cell factories DOI Creative Commons
Yuxi Teng, Tian Jiang, Yajun Yan

et al.

Trends in biotechnology, Journal Year: 2023, Volume and Issue: 42(1), P. 104 - 118

Published: July 26, 2023

Language: Английский

Citations

22
A self-regulated network for dynamically balancing multiple precursors in complex biosynthetic pathways DOI Creative Commons
Yusong Zou,

Jianli Zhang,

Jian Wang

et al.

Metabolic Engineering, Journal Year: 2024, Volume and Issue: 82, P. 69 - 78

Published: Feb. 3, 2024

Language: Английский

Citations

5
Advancing microbial production through artificial intelligence-aided biology DOI
Xinyu Gong, Jianli Zhang, Qi Gan

et al.

Biotechnology Advances, Journal Year: 2024, Volume and Issue: 74, P. 108399 - 108399

Published: June 24, 2024

Language: Английский

Citations

4
Recent advancements in flavonoid production through engineering microbial systems DOI
Yunhee Hwang, Myung Hyun Noh, Gyoo Yeol Jung

et al.

Biotechnology and Bioprocess Engineering, Journal Year: 2024, Volume and Issue: 29(5), P. 792 - 805

Published: June 27, 2024

Language: Английский

Citations

4
De novo biosynthesis of the 4,6-dihydroxycoumarin in Escherichia coli DOI Creative Commons
Qi Gan, Tian Jiang, Chenyi Li

et al.

Green Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

By combining protein engineering and synthetic biology strategies, the novel de novo biosynthesis of 4,6-dihydroxycoumarin from glycerol was realized in Escherichia coli for first time.

Language: Английский

Citations

0
Engineering an Overflow‐Responsive Regulation System for Balancing Cellular Redox and Optimizing Microbial Production DOI Creative Commons
Jianli Zhang, Jian Wang, Tian Jiang

et al.

Biotechnology and Bioengineering, Journal Year: 2025, Volume and Issue: unknown

Published: March 21, 2025

ABSTRACT Escherichia coli accumulates acetate as a byproduct in fast growth aerobic conditions when using glucose carbon source. This phenomenon, known overflow metabolism, has negative impacts on cell and protein expression, also causes loss during biosynthesis most microbial production scenarios. In this study, we regarded the “waste” metabolite useful metabolism indicator, constructed an biosensor to monitor change of concentration converted signal into various regulation outputs. Phloroglucinol is phenolic compound with several derivatives that exhibit pharmacological activities. By applying bifunctional dynamic system phloroglucinol production, released cellular redox pressure real‐time reduced waste flux metabolism. Finally, was redirected more favorably towards desired product, resulting boosted titer 1.30 g/L, increased by 2.04‐fold. Overall, study explored use central byproduct‐responsive improving metabolic status, providing general approach for enhancing bioproduction.

Language: Английский

Citations

0
Concentration Recognition‐Based Auto‐Dynamic Regulation System (CRUISE) Enabling Efficient Production of Higher Alcohols DOI Creative Commons
Zhenya Chen, Shengzhu Yu, Jing Liu

et al.

Advanced Science, Journal Year: 2024, Volume and Issue: 11(23)

Published: April 16, 2024

Abstract Microbial factories lacking the ability of dynamically regulating pathway enzymes overexpression, according to in situ metabolite concentrations, are suboptimal, especially when metabolic intermediates competed by growth and chemical production. The production higher alcohols (HAs), which hijacks amino acids (AAs) from protein biosynthesis, minimizes intracellular concentration AAs thus inhibits host growth. To balance resource allocation maintain stable AA flux, this work utilizes AA‐responsive transcriptional attenuator ivbL HA‐responsive activator BmoR establish a recognition‐based auto‐dynamic regulation system (CRUISE). This ultimately maintains homeostasis maximizes HA. It is demonstrated that ‐driven overexpression can regulate AA‐to‐HA conversion while BmoR‐driven accelerate biosynthesis during HA feedback activation mode. flux pathways balanced via concentration, vice versa stabilized competition between conversion. CRUISE, further aided scaffold‐based self‐assembly, enables 40.4 g L −1 isobutanol bioreactor. Taken together, CRUISE realizes robust sheds new light on dynamic control process

Language: Английский

Citations

3
Application of modern synthetic biology technology in aromatic amino acids and derived compounds biosynthesis DOI
Mi Tang,

Jiajia You,

Tianjin Yang

et al.

Bioresource Technology, Journal Year: 2024, Volume and Issue: 406, P. 131050 - 131050

Published: June 26, 2024

Language: Английский

Citations

3