Research Square (Research Square),
Journal Year:
2023,
Volume and Issue:
unknown
Published: Dec. 4, 2023
Abstract
The
global
marine
ecosystem
is
changing
rapidly
as
the
result
of
biogeochemical
cycles
and
structure
being
altered
by
industrial
civilization.
Invasive
species
(IMS)
are
one
most
damaging
regional
consequences
human
activity,
easily
attributable
to
specific
processes.
This
makes
IMS
introduction
tractable
threats
for
management
with
appropriate
policies.
Once
established,
a
different
set
policies
required
either
restrict
spread,
or
attempt
local
eradication.
key
tool
damage
mitigation
rapid,
widely
deployable
detection.
Environmental
Nucleic
Acids
(eNA),
combining
environmental
DNA
(eDNA)
RNA
(eRNA)
analyses,
have
emerged
valuable
tools
sensitive,
cost-effective
readily
detection
IMS.
Methods
eNA
still
developed
through
widespread
active
research
community,
so
identifying
limitations
current
processes
will
help
prioritise
eNA-based
research.
We
analysed
synthesised
opinions
expert
managers
researchers
in
Australia
New
Zealand
about
knowledge
gaps
needs
synthesis
was
placed
context
literature
on
what
technologies
currently
providing
an
tool;
problems
exist
technology;
could
be
done
improve
this
general
approach.
Our
analyses
produced
list
priorities
that
chart
path
towards
best
possible
systems
-
Utopia.
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2023,
Volume and Issue:
unknown
Published: June 3, 2023
Key
to
making
accurate
taxonomic
assignments
are
curated,
comprehensive
reference
barcode
databases.
However,
the
generation
and
curation
of
such
databases
has
remained
challenging
given
large
continuously
growing
volumes
DNA
sequence
data
novel
targets.
Monitoring
research
applications
require
a
greater
diversity
specialized
gene
regions
targeted
taxa
meet
classification
goals
then
currently
curated
by
professional
staff.
Thus,
there
is
need
for
an
easy
implement
tool
that
can
generate
metabarcoding
libraries
any
bespoke
locus.
We
address
this
reimagining
CRUX
from
Anacapa
Toolkit
present
rCRUX
package
in
R.
The
typical
workflow
involves
searching
plausible
seed
amplicons
(
Research Square (Research Square),
Journal Year:
2023,
Volume and Issue:
unknown
Published: Dec. 4, 2023
Abstract
The
global
marine
ecosystem
is
changing
rapidly
as
the
result
of
biogeochemical
cycles
and
structure
being
altered
by
industrial
civilization.
Invasive
species
(IMS)
are
one
most
damaging
regional
consequences
human
activity,
easily
attributable
to
specific
processes.
This
makes
IMS
introduction
tractable
threats
for
management
with
appropriate
policies.
Once
established,
a
different
set
policies
required
either
restrict
spread,
or
attempt
local
eradication.
key
tool
damage
mitigation
rapid,
widely
deployable
detection.
Environmental
Nucleic
Acids
(eNA),
combining
environmental
DNA
(eDNA)
RNA
(eRNA)
analyses,
have
emerged
valuable
tools
sensitive,
cost-effective
readily
detection
IMS.
Methods
eNA
still
developed
through
widespread
active
research
community,
so
identifying
limitations
current
processes
will
help
prioritise
eNA-based
research.
We
analysed
synthesised
opinions
expert
managers
researchers
in
Australia
New
Zealand
about
knowledge
gaps
needs
synthesis
was
placed
context
literature
on
what
technologies
currently
providing
an
tool;
problems
exist
technology;
could
be
done
improve
this
general
approach.
Our
analyses
produced
list
priorities
that
chart
path
towards
best
possible
systems
-
Utopia.