Decoding the Impact of a Bacterial Strain of Micrococcus luteus on Arabidopsis Growth and Stress Tolerance DOI Creative Commons

Yu-Cheng Chang,

P. A. Lee,

Chin‐Wang Hsu

et al.

Microorganisms, Journal Year: 2024, Volume and Issue: 12(11), P. 2283 - 2283

Published: Nov. 10, 2024

Microbes produce various bioactive metabolites that can influence plant growth and stress tolerance. In this study, a growth-promoting rhizobacterium (PGPR), strain S14, was identified as Micrococcus luteus (designated MlS14) using de novo whole-genome assembly. The MlS14 genome revealed major gene clusters for the synthesis of indole-3-acetic acid (IAA), terpenoids, carotenoids. produced significant amounts IAA, its volatile organic compounds (VOCs), specifically exhibited antifungal activity, suppressing pathogenic fungi. presence yellow pigment in bacterial colony indicated carotenoid production. Treatment with activated expression β-glucuronidase (GUS) driven by promoter containing auxin-responsive elements. application reshaped root architecture Arabidopsis seedlings, causing shorter primary roots, increased lateral growth, longer, denser hairs; these characteristics are typically controlled elevated exogenous IAA levels. positively regulated seedling enhancing photosynthesis, activating antioxidant enzymes, promoting production secondary reactive oxygen species (ROS) scavenging activity. Pretreatment reduced H2O2 malondialdehyde (MDA) levels seedlings under drought heat stress, resulting greater fresh weight during post-stress period. Additionally, exposure to stabilized chlorophyll content rate salt stress. transcriptionally upregulated genes involved defense photosynthesis. Furthermore, linked hormone signaling pathways, such abscisic (ABA), auxin, jasmonic (JA), salicylic (SA), displayed levels, those ABA synthesis, carotenoids precursors, being most highly induced. treatment several transcription factors associated responses, DREB2A showing highest level induction. conclusion, played roles Metabolites may function positive regulators metabolism pathways essential adaptation abiotic

Language: Английский

Microbial inheritance through seed: a clouded area needs to be enlightened DOI

Dibyendu Khan,

Rajdeep Shaw, Ashutosh Kabiraj

et al.

Archives of Microbiology, Journal Year: 2025, Volume and Issue: 207(1)

Published: Jan. 1, 2025

Language: Английский

Citations

1
Co-Inoculation of Phosphate-Solubilizing Bacteria and Rhizobia Increases Phosphorus Availability and Promotes the Development of Forage Legumes DOI Creative Commons
Winston Franz Ríos-Ruiz,

Rony Castro-Tuanama,

Renzo Alfredo Valdez-Nuñez

et al.

Agronomy, Journal Year: 2024, Volume and Issue: 14(11), P. 2493 - 2493

Published: Oct. 24, 2024

Tropical grassland soils, especially those with alkaline properties, often exhibit limited phosphorus availability due to its precipitation in insoluble forms. Phosphate-solubilizing bacteria (PSB) and rhizobia have demonstrated their potential enhance the of this nutrient promote growth forage legumes. This study, conducted under controlled conditions a mesh house, evaluated effect co-inoculation PSB, including Micrococcus sp. Sfcm-14-01, Agrobacterium Sfl-043-09, Enterobacter Sfcm-014-02 Sfcm-054-06, along (Ensifer terangae R1-012-02 Bradyrhizobium glycinis Rcm-025-01), different levels fertilization on legumes Leucaena leucocephala Centrosema macrocarpum. The results indicate significant increases various parameters, such as chlorophyll (SPAD), biomass (dry weight roots aerial parts) (mg), foliar concentration (ppm), available soil, particularly low-phosphorus conditions. highest level soil was achieved 75% recommended dose, resulting concentrations 13.73 ppm for L. 7.69 C. macrocarpum, representing 170.81% 240.27%, respectively, compared no or inoculation. These findings suggest that PSB native is promising strategy productivity mineral content tropical grazing systems, phosphorus-limited

Language: Английский

Citations

4
Isolation and characterization of root nodule-associated bacteria from chickpea (Cicer arietinum) for evaluating plant growth-promoting activities and heavy metal tolerance in West Bengal, India DOI
Sudipta Majhi, Mausumi Sikdar

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: April 22, 2025

Abstract Two bacterial strains were isolated from root nodules of the chickpea plant (Cicer arietinum) in West Bengal and characterized to assess their potential for heavy metal (HM) tolerance growth-promoting (PGP) attributes. Phylogenetic analysis based on 16S rRNA gene identified these as belonging genera Enterobacter sp. Labrys Chromium (Cr) was selected this experiment. Endophytic bacteria, known PGP abilities, assessed sustain traits under Cr (VI) stress. Bacterial strains, SMAJ_63 SMAJ_180, evaluated HM tolerance, with IC50 values 500 µM 270 (VI), respectively. attributes, including indole acetic acid (IAA) production phosphate solubilization, determined, strain exhibiting IAA 23.29 µg/mL. Additionally, MIC determined other metals such arsenic (As), cadmium (Cd), cobalt (Co), copper (Cu), well salinity drought Among tested metals, Cd exhibited highest toxicity, while Cu least toxic. The overall toxicity order different HMs is follows: > Co As both strains. At 50 Cu, promoted growth SMAJ_180 SMAJ_63, Furthermore, demonstrated ability produce exopolysaccharides (EPS) 0.67 g/L, which play a crucial role stress soil health improvement.

Language: Английский

Citations

0
Genome sequencing, annotation and application of a strain of Microbacterium paraoxydans – A bacterium with arsenic bioremediation and plant growth promoting potential DOI Creative Commons
Debjani Mandal,

Sadhan Kr. Das,

Jishnu Adhikari

et al.

The Microbe, Journal Year: 2024, Volume and Issue: 4, P. 100132 - 100132

Published: July 17, 2024

A heavy metal hypertolerant plant growth-promoting bacterium was isolated from the arsenic-contaminated garden soil of Bhagobangola I block (Murshidabad district). Metagenomic classification identified bacterial isolate as a member genus Microbacterium. Taxonomic assessment strain revealed its maximum average nucleotide identity (89.99 %) with Microbacterium paraoxydans DSM 15019. Prokaryotic genome annotation performed by Prokka, DFAST and RAST. The entire consisted 3365911 bases 69.90 % GC content. Prokka detected 3216 coding sequences (1461 hypothetical sequences), 3283 genes, 10 miscellaneous RNAs, 3 rRNAs, 53 tRNAs 1 tmRNA in genome. 3257 (1217 rRNA tRNA RAST 3285 49 RNAs Only 25 these could be categorised subsystems. In RAST, 789 32 were considered non-hypothetical hypothetical, respectively. genes gene clusters responsible for arsenic resistance (arsR, arsB, arsC, acr1, acr2 acr3), tolerance to other metals (copper, manganese, zinc, etc.) growth promotion (auxin biosynthesis, siderophore-mediated iron acquisition, phosphate polyphosphate metabolism, trehalose also bioremediation potential nature confirmed silver diethyl dithio carbamate (SDDC) method pot experiments, aforementioned traits point towards tool biofertilizer reducing toxicity promoting growth.

Language: Английский

Citations

3
Revealing the defluoridation efficacy of a ureolytic bacterium Micrococcus yunnanensis MLN22 through MICP driven biomineralization for sustainable groundwater development DOI

Moitri Let,

Krishnendu Majhi, Ayan De

et al.

Environment Development and Sustainability, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 14, 2024

Language: Английский

Citations

2
Mitigation of Salinity Stress on Vetiver Grass (Vetiveria zizanioides) through Application of Micrococcus yunnanensis and Indole-3-Acetic Acid DOI Creative Commons

Negar Mosallanejad,

Mehdi Zarei,

Reza Ghasemi‐Fasaei

et al.

Agronomy, Journal Year: 2024, Volume and Issue: 14(9), P. 1952 - 1952

Published: Aug. 29, 2024

Salinity represents an ever-challenging problem of agriculture in arid and semi-arid regions. This is considered a key limiting factor agricultural production the countries Southwest Asia. In recent years, use alternative methods chemical fertilizers has emerged as promising approach to mitigate negative effects salinity on crop yield. this research, effect Micrococcus yunnanensis indole-3-acetic (IAA) acid growth composition Vetiver grass (Vetiveria zizanioides) under salt stress been investigated. Based results, application IAA, M. their interaction significantly increased average plant growth, fresh dry weight aerial parts root weight. Considering properties plant, between IAA shoot phosphorus, potassium sodium absorption. Proline content, catalase, superoxide dismutase peroxidase activity were influenced by interaction. Follow-up experiments after vetiver harvest showed that treatments improved soil microbial biomass respiration. total, 34% activities enzymes decreased −20.61, −4.70 −8.00%, respectively, which shows pressure decreased. study reinforces previous literature positive biological improve performance providing new evidence mitigating salinity.

Language: Английский

Citations

1
Multimodal cadmium resistance and its regulatory networking in Pseudomonas aeruginosa strain CD3 DOI Creative Commons
Soumya Chatterjee, Partha Barman, Chandan Barman

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Dec. 30, 2024

Cadmium, a toxic heavy metal, poses significant global concern. A strain of the genus Pseudomonas, CD3, demonstrating cadmium resistance (up to 3 mM CdCl

Language: Английский

Citations

1
Isolation and Characterization of Arsenic-Tolerable Bacteria from Groundwater and Their Implementation on Rice Seedling’s Shoot and Root Enhancement DOI
Ashutosh Kabiraj, Urmi Halder, Rajib Bandopadhyay

et al.

Current Microbiology, Journal Year: 2024, Volume and Issue: 81(12)

Published: Oct. 24, 2024

Language: Английский

Citations

0
Decoding the Impact of a Bacterial Strain of Micrococcus luteus on Arabidopsis Growth and Stress Tolerance DOI Creative Commons

Yu-Cheng Chang,

P. A. Lee,

Chin‐Wang Hsu

et al.

Microorganisms, Journal Year: 2024, Volume and Issue: 12(11), P. 2283 - 2283

Published: Nov. 10, 2024

Microbes produce various bioactive metabolites that can influence plant growth and stress tolerance. In this study, a growth-promoting rhizobacterium (PGPR), strain S14, was identified as Micrococcus luteus (designated MlS14) using de novo whole-genome assembly. The MlS14 genome revealed major gene clusters for the synthesis of indole-3-acetic acid (IAA), terpenoids, carotenoids. produced significant amounts IAA, its volatile organic compounds (VOCs), specifically exhibited antifungal activity, suppressing pathogenic fungi. presence yellow pigment in bacterial colony indicated carotenoid production. Treatment with activated expression β-glucuronidase (GUS) driven by promoter containing auxin-responsive elements. application reshaped root architecture Arabidopsis seedlings, causing shorter primary roots, increased lateral growth, longer, denser hairs; these characteristics are typically controlled elevated exogenous IAA levels. positively regulated seedling enhancing photosynthesis, activating antioxidant enzymes, promoting production secondary reactive oxygen species (ROS) scavenging activity. Pretreatment reduced H2O2 malondialdehyde (MDA) levels seedlings under drought heat stress, resulting greater fresh weight during post-stress period. Additionally, exposure to stabilized chlorophyll content rate salt stress. transcriptionally upregulated genes involved defense photosynthesis. Furthermore, linked hormone signaling pathways, such abscisic (ABA), auxin, jasmonic (JA), salicylic (SA), displayed levels, those ABA synthesis, carotenoids precursors, being most highly induced. treatment several transcription factors associated responses, DREB2A showing highest level induction. conclusion, played roles Metabolites may function positive regulators metabolism pathways essential adaptation abiotic

Language: Английский

Citations

0