Inhibition of SARS-CoV-2-Induced NLRP3 Inflammasome-Mediated Lung Cell Inflammation by Triphala-Loaded Nanoparticle Targeting Spike Glycoprotein S1

Pharmaceutics, Journal Year: 2024, Volume and Issue: 16(6), P. 751 - 751

Published: June 2, 2024

The COVID-19 pandemic, caused by SARS-CoV-2, poses a significant global health threat. spike glycoprotein S1 of the SARS-CoV-2 virus is known to induce production pro-inflammatory mediators, contributing hyperinflammation in patients. Triphala, an ancient Ayurvedic remedy composed dried fruits from three plant species—Emblica officinalis (Family Euphorbiaceae), Terminalia bellerica Combretaceae), and chebula Combretaceae)—shows promise addressing inflammation. However, limited water solubility its ethanolic extract impedes bioavailability. In this study, we aimed develop nanoparticles loaded with Triphala extract, termed “nanotriphala”, as drug delivery system. Additionally, investigated vitro anti-inflammatory properties nanotriphala major compounds, namely gallic acid, chebulagic chebulinic lung epithelial cells (A549) induced CoV2-SP. formulation was prepared using solvent displacement method. encapsulation efficiency determined be 87.96 ± 2.60% based on total phenolic content. terms release, exhibited biphasic release profile zero-order kinetics over 0–8 h. A549 were treated or active compounds then 100 ng/mL subunit (CoV2-SP). results demonstrate that acid are nanotriphala, which significantly reduced cytokine (IL-6, IL-1β, IL-18) suppressed expression inflammatory genes IL-18, NLRP3) (p < 0.05). Mechanistically, notably attenuated inflammasome machinery proteins (NLRP3, ASC, Caspase-1) conclusion, nanoparticle enhances stability exhibits against CoV2-SP-induction. This achieved suppressing mediators NLRP3 machinery. Thus, holds supportive preventive therapy for COVID-19-related chronic

Language: Английский

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Mechanistic elucidation of Ayurvedic formulation Triphala in managing gastrointestinal and neurological disorders through in silico approach

Discover Plants., Journal Year: 2025, Volume and Issue: 2(1)

Published: Jan. 15, 2025

Triphala is an Ayurvedic herbal formulation used to treat gastrointestinal disorders. The present study explored the underlying mechanisms of against integrated and neurological disorders through network pharmacology molecular docking. Components were screened using Dr. Duke's Phytochemical Ethnobotanical Database, KNApSAcK database, Indian Medicinal Plants, Phytochemistry Therapeutics database while target prediction was done SuperPred 3.0, followed by mapping with shared targets obtained from GeneCards DisGenet databases. STRING Cytoscape utilized for protein–protein protein-target interaction analyses further screening core clusters. GO, KEGG, Reactome enrichment analysis performed, top subject binding affinity evaluation major compounds. Based on ADME characteristics, seven components overlapped 104 genes STAT3, ESR1, HIF1A, MTOR, PPARG, MAP2K2, TLR4, SLC2A1, NFKB1, ESR1 revealed as interacting nodes interaction, compound-target compound-target-disease-pathway analysis. GO indicated involved in managing cell response stress, stimuli, metabolism KEGG pathways related cancer, metabolism, signalling. implied immune system, signalling, disease pathways. Thannilignan, 7-Hydroxy-3',4'-methylenedioxyflavan, Termilignan identified phytocompounds Triphala. Molecular docking excellent interactions all analysed receptors mean energies ranging − 7.58 8.34 kcal/mol/receptor which most notable compound 7-Hydroxy-3',4'-methylenedioxyflavan. Together, this work delineates identifies new drug candidates management

Language: Английский

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Levying evidence of the impact of Triphala in the mildly constipated human colon microbiota

Journal of Functional Foods, Journal Year: 2025, Volume and Issue: 125, P. 106698 - 106698

Published: Feb. 1, 2025

Language: Английский

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Drugs Discovery from Traditional Phytotherapy

Published: April 29, 2025

BACKGROUND. Phytotherapy refers to the use of plant extracts for therapeutic purpose. It plays important role in ancient medicine practices. has been considered as a cornerstone development modern drugs. With advancement field science and technology, researchers systematically evaluate medicinal properties plants. OBJECTIVE. is an integrative approach that integrates traditional knowledge with techniques identification, isolation, test derivative compounds. Literature on plant-derived natural products used suitable precursors drug discovery was included. METHODS. The data source were obtained through literature publications using different scientific search engines. RESULTS. These papers carefully evaluated, critically analyzed structured accurate information. process begins ethnobotanical surveys study select promising potential herb. Phytochemical methods are then employed extract isolate active constituents. phytotherapy drugs have plants support health manage various conditions. Recently analytical chemistry genomics essential accelerating process. High-throughput screening, molecular docking, bioinformatics tools enabled rapid identification bioactive Traditional significantly influenced by providing wealth about plant-based remedies. Many pharmaceuticals roots herbal practices, compounds isolated from forming basis effective treatments. CONCLUSION. This integration wisdom research led targeted therapies, emphasizing importance discovery. As continue explore compounds, remains vital new medicines. Moreover, advancements pharmacokinetics pharmacodynamics improved understanding interact biological systems, enhancing their efficacy safety profiles.

Language: Английский

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Pomegranate polyphenol punicalagin as a nutraceutical for mitigating mild cognitive impairment: An overview of beneficial properties

European Journal of Pharmacology, Journal Year: 2024, Volume and Issue: 977, P. 176750 - 176750

Published: June 17, 2024

Language: Английский

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The adverse and beneficial effects of polyphenols in green and black teas in vitro and in vivo

International Journal of Medical Sciences, Journal Year: 2023, Volume and Issue: 20(10), P. 1247 - 1255

Published: Jan. 1, 2023

Although numerous studies highlight the health benefits of tea, excessive consumption has been linked to toxic conditions.Thus, understanding optimal tea is essential minimize toxicity while maximizing its benefits.In this study, we investigated effects eight green samples (G1-G8) and black (R1-R8) from Camellia sinensis, most popular teas in Asian culture, on RSC96 Schwann neural cells embryonic cardiomyocyte H9c2 cells.The results showed that IC50 (mg/ml, weight/volume) both types were inversely proportional their polyphenol content, suggesting a relationship between levels tea.Interestingly, generally have higher content than teas.We also assessed protective vitro by pretreating with at indicated doses subsequently exposing them H2O2.Both significantly reduced decline cell viability for lines, there was no significant difference concentrations (G3 & G7) (R3 R8) effective (EC20 EC40).To evaluate preventative vivo, examined impact two varying dextran sulfate sodium (DSS)-induced inflammatory colitis mice.Tea-treated groups exhibited lower scores (DAI) control group.DSS treatment group led shortened colorectal lengths mice, co-treatment partially prevented loss.Histological analysis revealed G7 R3 (with moderate content) improved crypt structure, decreased severity ulcerative colitis, histological compared group.However, G3 R8 high low respectively) did not show these effects, level benefits.

Language: Английский

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Phyllanthus emblica Linn. fruit polyphenols improve acute paradoxical sleep deprivation-induced cognitive impairment and anxiety via Nrf2 pathway

Journal of Functional Foods, Journal Year: 2023, Volume and Issue: 110, P. 105884 - 105884

Published: Nov. 1, 2023

Phyllanthus emblica Linn. fruit that primarily contains many polyphenols, have been considered homologous food for centuries and used as a remedy variety of ailments, including maintaining brain function. It is, however, unclear what is the mechanism protective action polyphenols (PEFPs) against cognitive impairment anxiety induced by acute paradoxical sleep deprivation (SD). Behavioral abnormalities SD were effectively alleviated PEFPs, which also prevented histopathological morphological damage to hippocampal neurons. Acute SD-induced impairments are treated PEFPs through modulating biological functional modules, such oxidative stress, inflammation/immune, cell proliferation differentiation, metabolism, neurotransmitter signaling. Further results showed markedly counteracted stress neuroinflammation activating Nrf2 pathway. In summary, attractive nutraceutical ingredients preventing associated with loss.

Language: Английский

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Protective and therapeutic role of Itrīfal (Unani dosage form) in neuro behavior, neurodegeneration, and immunomodulation: An appraisal

Brain Behavior and Immunity Integrative, Journal Year: 2024, Volume and Issue: 7, P. 100075 - 100075

Published: July 1, 2024

Itrīfal, an important dosage form of Unani medicine, was introduced some 1000 years ago for the treatment mainly brain disorders. This semi-solid is usually prepared by adding dried fruits E. officinalis, T. chebula, and bellirica, along with other herbs plant origin. The present review aimed at evaluating potential effects different formulations Itrīfal its ingredients respect to traditional uses scientific studies. carried out after going through classical literature related therapeutic various in cerebral diseases. data pharmacological activities biologically active compounds their were explored search engines like PubMed, Science Direct, Springer, MEDLINE, Research Gate, Google Scholar. revealed that eliminate phlegmatic, bilious, melancholic morbid humors produced because ihtirāq microcellular level cases phytochemical they contain bioactive secondary metabolites, including flavonoids, polyphenols, monoterpenes, alkaloids, glycosides, amino acids, tannins, etc., which possess several activities, such as antioxidant, neuroprotective, anxiolytic, antidepressant, anti-aging, memory enhancer, immunomodulatory, mechanisms actions inhibition acetylcholinesterase butrylcholinesterase enzymes, modulation neurotransmitters, interaction adrenergic, dopaminergic, serotonergic systems, blockade dopamine receptors enhancement GABAergic neurotransmission, neurogenesis, synaptogenesis, etc. has been rationale mixtures herbs, preventive curative They can provide much-needed protection nerves against oxidative stress disease-oriented factors. limitations this unique medicine are adequate studies have not on preparations holistic exploration extraordinary form. Hence, it suggested more aspects, pharmacological, toxicological, pharmacodynamics, pharmacokinetics, may be future.

Language: Английский

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Indian Ayurvedic medicine: Overview and application to brain cancer

Journal of Ayurveda and Integrative Medicine, Journal Year: 2024, Volume and Issue: 15(4), P. 101013 - 101013

Published: July 1, 2024

Ayurveda is the traditional medicine system of India, and has been in practice for millennia. It a approach that uses 1000's different plant preparations various combinations treatment human ailments, including cancer. Ethnopharmacological phytochemical analyses are now elucidating bioactive constituents species herbal formulations, ashwagandha, curcumin, guduchi, triphala, others. To provide an overview of: 1) ethnopharmacology several its most important pharmacological molecular mechanisms anti-cancer effects; 2) review literature applying Ayurvedic herbs formulations to brain tumors. A detailed PubMed search was performed included publications involving Ayurveda, cancer, ethnopharmacology, analysis, In recent decades, significant research begun elucidate compounds tumeric, such as withaferin A, withanolides, palmatine, many These extracts being applied tumor cells vitro animal models, with positive signs activity reduced cell growth, increased apoptosis, cycle arrest, differentiation, inhibition internal signal transduction pathways. Several (ashwagandha, curcumin) have activity, effective early pre-clinical testing against models. Further warranted, along advancement into phase I II clinical trials patients glioblastoma other

Language: Английский

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A study on the improvement of lipid metabolism disorders in the treatment of hyperprolactinemia rats with liver-depression by Sini San based on AKT1/TNF/IL6 signaling pathway

Current Research in Food Science, Journal Year: 2024, Volume and Issue: 9, P. 100853 - 100853

Published: Jan. 1, 2024

Language: Английский

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