This
study
aimed
to
compare
the
proliferation,
differentiation
rates,
and
related
gene
expression
levels
of
skeletal
muscle
satellite
cells
(SMSCs)
isolated
from
Hanwoo
steers
cows
identify
a
suitable
source
for
cell-based
meat
production.
SMSCs
were
extracted
three
cows,
their
cell
growth
doubling
times,
genetic
differences
determined.
Comparative
analysis
revealed
that
exhibited
higher
rate
than
cows.
Doubling
time
was
shorter
in
(p<0.0001).
During
differentiation,
fusion
index
showed
significant
between
Steers
after
24
h,
72
h.
Genetic
level
Pax7
significantly
(p<0.001).
The
differentiation-related
genes,
including
MyoG,
MRF4,
MHC1
Thus,
steer
exhibit
faster
rate,
these
findings
are
expected
provide
valuable
information
determining
optimal
food.
Food Science of Animal Resources,
Journal Year:
2024,
Volume and Issue:
44(2), P. 326 - 355
Published: Feb. 2, 2024
Expectations
for
the
industrialization
of
cultured
meat
are
growing
due
to
increasing
support
from
various
sectors,
such
as
food
industry,
animal
welfare
organizations,
and
consumers,
particularly
vegetarians,
but
progress
is
slower
than
initially
reported.
This
review
analyzes
main
issues
concerning
meat,
examines
research
media
reports
on
development
date,
presents
current
technology,
level,
prospects
meat.
Currently,
over
30
countries
have
companies
industrializing
around
200
that
developing
or
been
surveyed
globally.
By
country,
United
States
has
50
companies,
accounting
more
20%
total.
Acquiring
cells,
cell
lines,
improving
proliferation,
efficiency
differentiation
muscle
production,
culture
media,
including
serum-free
major
themes
related
In
contrast,
devices,
bioreactors,
which
crucial
in
enabling
large-scale
relatively
understudied,
few
many
invested
presented
products
sale
other
prototypes.
addition,
because
most
information
key
technologies
not
publicly
available,
it
possible
determine
level
technology
surmised
meat-related
startups
high.
Therefore,
further
needed
promote
full-scale
animal,
Journal Year:
2024,
Volume and Issue:
unknown, P. 101242 - 101242
Published: July 1, 2024
With
the
current
environmental
impact
of
large-scale
animal
production
and
societal
concerns
about
welfare
farm
animals,
researchers
are
questioning
whether
we
can
cultivate
cells
for
purpose
food
production.
This
review
focuses
on
a
pivotal
aspect
cellular
agriculture
domain:
cells.
We
summarised
information
various
cell
types
from
animals
currently
used
development
cultured
meat,
including
mesenchymal
stromal
cells,
myoblasts,
pluripotent
stem
The
delves
into
advantages
limitations
each
type
considers
factors
like
selection
appropriate
source,
as
well
culture
conditions
that
influence
performance.
As
research
in
meat
seeks
to
create
muscle
fibers
mimic
texture
nutritional
profile
focused
myogenic
differentiation
capacity
most
commonly
this
myoblasts
or
satellite
but
given
their
limited
proliferation
capacity,
efforts
underway
formulate
protocols
multipotent
character
latter
might
enable
creation
other
tissues
found
such
adipose
connective
tissues.
help
guiding
context
development.
Food Science of Animal Resources,
Journal Year:
2024,
Volume and Issue:
44(3), P. 499 - 514
Published: Feb. 29, 2024
Cultured
meat
has
been
gaining
popularity
as
a
solution
to
the
increasing
problem
of
food
insecurity.
Although
research
on
cultured
started
later
compared
other
alternative
meats,
industry
is
growing
rapidly
every
year,
with
developed
products
evaluated
being
most
similar
conventional
meat.
Studies
production
techniques,
such
culturing
new
animal
cells
and
developing
medium
sera
scaffolds,
are
conducted
intensively
diversely.
However,
active
in-depth
quality
characteristics
meat,
including
studies
sensory
storage
properties
that
directly
influence
consumer
preferences,
still
lacking.
Additionally,
combination
or
ratio
fat
muscle
improvement
microbiota,
protein
degradation,
fatty
acid
degradation
remain
be
conducted.
By
actively
investigating
these
topics,
we
aim
verify
safety
ultimately
improving
preference
for
products.
Food Science of Animal Resources,
Journal Year:
2024,
Volume and Issue:
44(5), P. 1167 - 1180
Published: Aug. 7, 2024
This
study
aimed
to
investigate
effects
of
chicken
age
on
proliferation
and
differentiation
capacity
muscle
satellite
cells
(MSCs)
determine
total
amino
acid
contents
cultured
meat
(CM)
produced.
Chicken
MSCs
(cMSCs)
were
isolated
from
hindlimb
muscles
broiler
chickens
at
5-week-old
(5W)
19-embryonic-day
(19ED),
respectively.
Proliferation
abilities
(population
doubling
time
cell
counting
kit
8)
cMSCs
19ED
significantly
higher
than
those
5W
(p
<
0.05).
Likewise,
both
myotube
formation
area
expression
myosin
heavy
chain
After
serially
subcultured
for
long-term
cultivation
in
2D
flasks
produce
tissue
(CMT),
CMT
showed
no
significant
difference
between
>
finding
suggests
that
embryos
are
more
suitable
improving
the
production
efficiency
CM
derived
young
chickens.
Animals,
Journal Year:
2024,
Volume and Issue:
14(5), P. 819 - 819
Published: March 6, 2024
Cultured
meat
production
relies
on
various
cell
types,
including
muscle
stem
cells
(MuSCs),
embryonic
lines,
induced
pluripotent
and
naturally
immortalized
lines.
MuSCs
possess
superior
differentiation
capabilities
compared
to
the
other
three
making
them
key
for
cultured
development.
Therefore,
produce
using
MuSCs,
they
must
first
be
effectively
separated
from
muscles.
At
present,
methods
used
isolate
muscles
include
(1)
pre-plating
method,
ability
of
adhere
differently,
which
is
a
biological
characteristic
MuSCs;
(2)
density
gradient
centrifugation
intrinsic
difference
cells,
physical
(3)
fluorescence-
magnetic-activated
sorting
methods,
surface
marker
protein
molecular
MuSCs.
Further
efficient
valuable
separating
are
expected
required
as
cell-based
industry
develops.
Thus,
we
take
closer
look
at
four
currently
in
use
discuss
future
development
directions
this
review.
PLoS ONE,
Journal Year:
2025,
Volume and Issue:
20(4), P. e0321650 - e0321650
Published: April 16, 2025
Muscle
satellite
cell
(MSC)
isolation,
proliferation,
and
differentiation
are
the
basis
of
cultured
meat
(CM)
technology,
which
emerged
as
a
sustainable
moral
substitute
for
conventional
animal
agriculture.
Notwithstanding
encouraging
future
CM,
there
still
lot
obstacles
to
overcome,
like
high
expense
culture
media
need
fetal
bovine
serum
(FBS).
The
goal
this
work
is
determine
whether
plant-based
nitrogen
source
soy
protein
hydrolysate
(SPH)
can
improve
myogenic
functional
development
in
MSCs
CM
by
acting
substitute.
We
concentrated
on
how
Angel
Yeast
Company’s
SPH
PU041
affected
C2C12
mouse
line,
useful
model
studying
muscle
biology
sector.
Adding
containing
different
concentrations
FBS
was
found
promote
proliferation
elongation,
with
optimal
effects
observed
at
0.5
g/L.
Immunofluorescence
flow
cytometry
analyses
revealed
that
up-regulated
levels
myosin
heavy
chain
(MyHC)
factor
1
(MyoD),
key
biomarkers
myogenesis.
Furthermore,
quantitative
real-time
PCR
(qPCR)
confirmed
up-regulation
MyHC,
MyoD,
myogenin
(MyoG)
mRNA
expression,
indicating
induces
differentiation.
findings
suggest
potentially
be
used
reduce
costs
associated
production
viable
substitute,
thereby
facilitating
more
ethical
approach
food
production.
However,
precise
mechanisms
underlying
PU041’s
warrant
further
investigation.
Animals,
Journal Year:
2024,
Volume and Issue:
14(7), P. 1091 - 1091
Published: April 3, 2024
This
study
evaluates
a
new
multiport
device
with
single
access
to
the
abdominal
cavity
produced
routine
hospital
supplies
that
could
be
applied
laparoscopically
assisted
cryptorchidectomy
in
standing
horses.
Initially,
was
evaluated
on
five
cadavers
of
bovine
fetuses
(n
=
5),
placed
minilaparotomy
performed
flank
region.
Subsequently,
four
cryptorchid
horses
treated
during
routine.
During
evaluation
device,
possibilities
exploring
cavity,
inspection,
and
intra-abdominal
manipulation
two
Babcock
forceps
were
verified.
The
described,
surgical
time
data
recorded
analyzed
using
descriptive
statistics.
In
cadavers,
wide
exploration
possible,
laparoscopic
inspection
through
right
paralumbar
fossa
structures
inserted
by
device.
horses,
feasible
patients,
others,
it
allowed
diagnosis
adhesions
ectopic
locations
inguinal
region
testicles
retained
cavity.
Therefore,
efficient
position.
present
is
preliminary
can
support
future
studies
aim
improve
developed
prototype.
