Development of a Novel Japanese Eel Myoblast Cell Line for Application in Cultured Meat Production DOI Creative Commons
Daisuke Ikeda,

Yui Otsuka,

Nobuhiro Kanno

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 10, 2024

Abstract The present study investigates the isolation, analysis, and characterization of primary cultured cells derived from muscle tissue Japanese eel ( Anguilla japonica ), culminating in establishing a spontaneously immortalized myoblast cell line, JEM1129. We isolated satellite to establish foundation for meat production. While initial cultures contained myoblasts, continued passaging led decline characteristics an increase fibroblast-like cells. RNA-Seq RT-qPCR analyses showed significant downregulation well-established markers such as pax7a myoD, over successive passages, highlighting loss myoblastic traits. Single-cell cloning was employed overcome this challenge maintain purity, leading successful creation JEM1129 line. These demonstrated enhanced expression marker genes, exceeding culture population. strong myotube formation, particularly when differentiation medium, indicating their robust potential development. line represents advancement cultivation cells, offering promising avenue findings contribute deeper understanding biology provide valuable insights into using fish-derived myoblasts Highlights established stable aids fish myoblasts. demonstrates myogenic differentiation, crucial meat. Potential sustainable production reducing wild depletion.

Language: Английский

MSTN knockout enhances the production of MYOD1-mediated steak-type cultivated meat DOI Creative Commons

Kyeong-Hyeon Eom,

Dayi Jeong, Jae-yoon Choi

et al.

Journal of Animal Science and Biotechnology/Journal of animal science and biotechnology, Journal Year: 2025, Volume and Issue: 16(1)

Published: March 11, 2025

Abstract Background As the global population increases, demand for protein sources is expected to increase, driving cell-based cultivated meat. This study aimed enhance productivity of meat through optimization cell source and organization process. Results We engineered fibroblasts into myogenic cells via non-viral introduction MYOD1 gene, avoiding viral methods safety. After confirming stable derivation cells, we combined knockout (KO) MSTN , a negative regulator myogenesis, with -mediated myogenesis improve production. Primary from KO cattle exhibited enhanced potential. Additionally, when tested in immortalized fibroblasts, myostatin treatment reduced -induced two-dimensional cultures, while increased it. To achieve muscle-like alignment, employed digital light processing (DLP)-based three-dimensional (3D) bioprinting organize 3D groove-shaped hydrogels. These bioactive hydrogels supported proliferation significantly improved muscle alignment. Upon differentiation myotubes, demonstrated an ordered particularly which showed highly efficient differentiation. Conclusions The integration genetic modification advanced DLP groove-patterned provides effective strategy producing high-quality, muscle-aligned

Language: Английский

Citations

1
Development of a Novel Japanese Eel Myoblast Cell Line for Application in Cultured Meat Production DOI
Daisuke Ikeda,

Yui Otsuka,

Nobuhiro Kanno

et al.

Biochemical and Biophysical Research Communications, Journal Year: 2024, Volume and Issue: 734, P. 150784 - 150784

Published: Oct. 2, 2024

Language: Английский

Citations

4
Exploring Aneuploidies in Two-Center Isolated Bovine Embryonic Stem Cell Lines: Implications for Cultured Meat Production DOI Creative Commons
Chloë Deelkens, Elly De Vlieghere, Mario Van Poucke

et al.

Future Foods, Journal Year: 2025, Volume and Issue: unknown, P. 100555 - 100555

Published: Jan. 1, 2025

Language: Английский

Citations

0
Editorial: Challenges of food and feed for the future DOI Creative Commons

René Baumont,

Diego Morgavi, Annemarie P. van Wezel

et al.

animal, Journal Year: 2025, Volume and Issue: 19, P. 101418 - 101418

Published: Feb. 1, 2025

Language: Английский

Citations

0
Isolation and purification of different high-purity cell populations from pig muscle tissue DOI Creative Commons
Zenan Hu, Zheng Liu, Renpeng Guo

et al.

Food Materials Research, Journal Year: 2025, Volume and Issue: 5(1), P. 0 - 0

Published: Jan. 1, 2025

Language: Английский

Citations

0
Evaluation of enzymatic protocols to optimize efficiency of bovine adipose tissue-derived mesenchymal stromal cell isolation DOI Creative Commons

Emma Heyman,

Bert Devriendt, Elly De Vlieghere

et al.

npj Science of Food, Journal Year: 2024, Volume and Issue: 8(1)

Published: Oct. 1, 2024

Language: Английский

Citations

2
Cattle, swine, poultry and fish cells for meat production: addressing challenges of co-culture and the innovative approaches for muscle and fat integration DOI

Milena Dutra Pierezan,

Silvani Verruck

Current Opinion in Food Science, Journal Year: 2024, Volume and Issue: 60, P. 101230 - 101230

Published: Oct. 22, 2024

Language: Английский

Citations

2
Development of a Novel Japanese Eel Myoblast Cell Line for Application in Cultured Meat Production DOI Creative Commons
Daisuke Ikeda,

Yui Otsuka,

Nobuhiro Kanno

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 10, 2024

Abstract The present study investigates the isolation, analysis, and characterization of primary cultured cells derived from muscle tissue Japanese eel ( Anguilla japonica ), culminating in establishing a spontaneously immortalized myoblast cell line, JEM1129. We isolated satellite to establish foundation for meat production. While initial cultures contained myoblasts, continued passaging led decline characteristics an increase fibroblast-like cells. RNA-Seq RT-qPCR analyses showed significant downregulation well-established markers such as pax7a myoD, over successive passages, highlighting loss myoblastic traits. Single-cell cloning was employed overcome this challenge maintain purity, leading successful creation JEM1129 line. These demonstrated enhanced expression marker genes, exceeding culture population. strong myotube formation, particularly when differentiation medium, indicating their robust potential development. line represents advancement cultivation cells, offering promising avenue findings contribute deeper understanding biology provide valuable insights into using fish-derived myoblasts Highlights established stable aids fish myoblasts. demonstrates myogenic differentiation, crucial meat. Potential sustainable production reducing wild depletion.

Language: Английский

Citations

0